Engineered for controlled polymerase activity, Accuris Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.
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Two-tube formulation: Hot Start Taq DNA Polymerase & 5X buffer
- Allows for reaction set-up at room temperature without the risk of non-specific amplification
- Optimized buffer for fast cycling & reproducibility
- High performance with gDNA & GC-rich sequences
- Exceptional sensitivity for low copy PCR
- Ideal for multiplex PCR and amplification of GC-rich DNA.
- Same enhanced features as Accuris Taq Polymerase
- Buffer optimized for fast cycling and reproducibility
Each 500 unit kit includes:
- 1 tube of Hot Start Taq DNA Polymerase (5 U/μL)
- 4 tubes of 5X buffer (PCR enhancers & 15mM MgCl2)
Applications:
- Colony PCR
- Fast REal-Time PCR
- GC-rich DNA Amplification
- Low Copy PCR
- Multiplex PCR
Engineered for controlled polymerase activity, Accuris Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.
When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard Accuris Taq, Hot Start Taq is provided with a 5X buffer, or in a ready to use 2X Master Mix. The Master Mix can be ordered with or without an incorporated red gel loading dye. The Red Dye Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel. The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.
