β-Galactosidase Antibody – Catalog Number: B2014930 (50 ug)

β-Galactosidase Antibody is offered in a defined 50 ug package (Catalog B2014930) with a listed 200 µg/ml value under “Molecular Weight or Concentration” and storage noted as 2–8oC. The supplier positions this reagent as a molecular tool for various biochemical applications. Because the listing does not specify format details beyond “Supplied as: N/A,” this entry is best treated as a clearly documented antibody item whose core attributes—amount, concentration field, storage, and keyword identity—facilitate straightforward SOP inclusion and inventory control in multi-user labs.

In common research workflows, antibodies to β-galactosidase are used to verify the presence of β-gal in samples tied to reporter systems or enzyme preparations, assemble capture/detection steps in qualitative immunoassays, or create control arms for training and platform checks. When the vendor does not prescribe a working dilution, it is sensible to determine conditions empirically for the matrix and platform at hand. Start with small pilot titrations, document buffer composition, incubation times, and time-at-temperature, and record lot identifiers so results are reproducible across runs and analysts. The concise specification set on this listing supports those documentation habits and helps teams harmonize handling across plates, membranes, or other bench setups.

Practical considerations include confirming background with the intended blocking and wash buffers, aligning secondary detection chemistry to the antibody’s characteristics used in your lab, and tracking any stability notes under the stated 2–8oC storage. Within research-only boundaries, the clearly stated package size, the presence of a concentration entry, and the minimal but explicit application note make this β-galactosidase antibody a straightforward, dependable component for labs standardizing β-gal-related immunochemical workflows and exploratory assay development.

Why researchers choose this product:

• Defined 50 ug package size for accurate documentation and inventory planning
• Listing includes a 200 µg/ml concentration entry to aid method setup and recording
• Specified storage at 2–8oC aligns with routine refrigerator handling
• Positioned as a molecular tool suitable for diverse biochemical workflows
• Concise, documentation-friendly fields (amount, concentration entry, storage, keywords, grade)

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

• 1: Cho EW, Lee MK, Kim KL, Hahm KS. Binding kinetics of monoclonal antibody using antigen-beta-galactosidase hybrid protein: application to measurement of peptide antigenicity J Immunoassay. 1995 Nov;16(4):349-63.
• 2: Welply JK, Fowler AV, Zabin I. beta-Galactosidase alpha-complementation. Overlapping sequences J Biol Chem. 1981 Jul 10;256(13):6804-10.
• 3: Grage K, Rehm BH. In vivo production of scFv-displaying biopolymer beads using a self-assembly-promoting fusion partner Bioconjug Chem. 2008 Jan;19(1):254-62.
• 4: Durbin H, Bodmer WF. A sensitive micro-immunoassay using beta-galactosidase/anti-beta-galactosidase complexes J Immunol Methods. 1987 Feb 26;97(1):19-27.
• 5: Franco D, de Boer PA, de Gier-de Vries C, Lamers WH, Moorman AF. Methods on in situ hybridization, immunohistochemistry and beta-galactosidase reporter gene detection Eur J Morphol. 2001 Jul;39(3):169-91.
• 6: Notkins AL. Polyreactive antibodies and polyreactive antigen-binding B (PAB) Cells Curr Top Microbiol Immunol. 2000;252:241-9.
• 7: Ishikawa E, Imagawa M, Hashida S, Yoshitake S, Hamaguchi Y, Ueno T. Enzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining J Immunoassay. 1983;4(3):209-327.
• 8: Yu Y, Fang L, Sun D. Biodistribution of HuCC49DeltaCH2-beta-galactosidase in colorectal cancer xenograft model Int J Pharm. 2010 Feb 15;386(1-2):208-15.
• 9: Franco D, de Boer PA, de Gier-de Vries C, Lamers WH, Moorman AF. Methods on in situ hybridization, immunohistochemistry and beta-galactosidase reporter gene detection Eur J Morphol. 2001 Feb;39(1):3-25.
• 10: Brigati JR, Petrenko VA. Thermostability of landscape phage probes Anal Bioanal Chem. 2005 Jul;382(6):1346-50. pubmed.ncbi.nlm.nih.gov