Ethylenediaminetetraacetic acid (EDTA) – Catalog Number: B2010688 (50 g)

Ethylenediaminetetraacetic acid (EDTA) is provided as a biotechnology-grade powder in a defined 50 g fill (Catalog B2010688) with RT storage and a listed molecular weight of 292.24 g/mol. The supplier identifies EDTA as a highly pure hydrophilic metal chelating agent and notes its use as a molecular tool in the formulation of a wide variety of reagents. In practice, disodium-free EDTA acid is widely adopted where controlled chelation of metal ions is important for buffer preparation, method development, and training exercises. Clear documentation fields—amount, supplied form, storage, keywords, and biotechnology-grade note—support straightforward SOP integration and inventory traceability in multi-user labs.

EDTA’s chelating ability helps manage the availability of divalent and trivalent cations during research workflows. By binding metal ions in solution, investigators can stabilize components that are sensitive to adventitious metals, shape enzyme behavior in exploratory assays, and assemble pilot formulations intended to reduce background. Because the listing does not prescribe a working concentration, empiric determination is recommended: prepare small test stocks, record pH and ionic strength of the final buffer system, and document incubation times and temperature histories. Tracking lot identifiers and time-at-temperature assists with reproducibility across operators and instruments and provides a clean path for method transfer and future audits.

Common research scenarios include building chelator-containing buffers for nucleic acid or protein handling; configuring reagent panels to explore how metal ions influence qualitative assay performance; and drafting instructional demonstrations where controlled chelation produces an interpretable change in signal. A powder presentation enables precise mass-based dosing and flexible solvent choice. The biotechnology-grade statement—solutions prepared using Type I ultrapure water and fine filtration—aligns with expectations for low background during optimization. Within research-only boundaries, this clearly specified EDTA offering integrates smoothly into projects that benefit from consistent chelation behavior and well-documented reagent handling.

Why researchers choose this product:

• Defined 50 g package in powder form for precise dosing and stock preparation
• Listed as a hydrophilic metal chelating agent and a molecular tool for reagent formulation
• 292.24 g/mol entry supports clear record-keeping and calculations
• Specified RT storage simplifies inventory management
• Biotechnology-grade note (Type I water; 0.22 µm filtration for solutions) aligns with low-background expectations
• Documentation-friendly keywords and concise fields streamline SOP/LIMS mapping

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

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• 2: Vagace JM, Rodriguez MÁ, de la Maya MD, Gervasini G. Ethylenediaminetetraacetic acid-dependent pseudomacrocytosis J Clin Pathol. 2013 Sep;66(9):811-4.
• 3: Ross EH, Dickinson A. Dilutional Effect of Ethylenediaminetetraacetic Acid on Packed Cell Volume in Healthy Dogs J Am Anim Hosp Assoc. 2021 Sep 1;57(5):199-204.
• 4: Chen K, Li P, Zhu C, Xia Z, Xia Q, Zhong L, Xiao B, Cheng T, Wu C, Shen C, Zhang X, Zhu J. Mn(II) Complex of Lipophilic Group-Modified Ethylenediaminetetraacetic Acid (EDTA) as a New Hepatobiliary MRI Contrast Agent J Med Chem. 2021 Jul 8;64(13):9182-9192.
• 5: Hülsmann M, Heckendorff M, Lennon A. Chelating agents in root canal treatment: mode of action and indications for their use Int Endod J. 2003 Dec;36(12):810-30.
• 6: Sarker RR, Tsunoi Y, Haruyama Y, Ichiki Y, Sato S, Nishidate I. Combined Addition of Ethanol and Ethylenediaminetetraacetic Acid Enhances Antibacterial and Antibiofilm Effects in Methylene Blue-Mediated Photodynamic Treatment against Pseudomonas aeruginosa In Vitro Photochem Photobiol. 2021 May;97(3):600-606.
• 7: Déchelotte B, Girot H, Chagraoui A, Prevost G, Brunel V. Dipotassium ethylenediaminetetraacetic acid is better than tripotassium salt for electrochemiluminescence insulin measurement Clin Chim Acta. 2016 Dec 1;463:45-46.
• 8: Altan A. Accidental injection of ethylenediaminetetraacetic acid (EDTA) instead of an anaesthetic solution: a case report J Stomatol Oral Maxillofac Surg. 2020 Feb;121(1):77-79.
• 9: Shen XX, Sun Y, Huo LJ, She R, Lei YY. [Effect of 17% ethylenediaminetetraacetic acid irrigation on the dentin adhesion of Enterococcus faecalis] Hua Xi Kou Qiang Yi Xue Za Zhi. 2019 Aug 1;37(4):372-377.
• 10: Klein K, Gartlan B, Doden G, Fredrickson K, Adamovicz L, Allender MC. COMPARING THE EFFECTS OF LITHIUM HEPARIN AND DIPOTASSIUM ETHYLENEDIAMINETETRAACETIC ACID ON HEMATOLOGIC VALUES IN EASTERN BOX TURTLES (TERRAPENE CAROLINA CAROLINA) J Zoo Wildl Med. 2021 Jan;51(4):999-1006. pubmed.ncbi.nlm.nih.gov