Chicken anti-Green Fluorescent Antibody Biotin – Catalog #: B2016358 (100 μg)

Chicken anti-Green Fluorescent Antibody Biotin is a high-quality powder of biotin-labeled antibody directed against green fluorescent protein (GFP), supplied in a convenient 100 μg format. As a biotech-grade reagent manufactured with ultrapure water and fine filtration, it offers high purity suitable for sensitive immunological and biochemical assays. The lyophilized form simplifies handling and allows flexible reconstitution depending on downstream application needs, while the biotin tag enables rapid capture, detection, or conjugation in affinity-based workflows.

This antibody serves as a versatile molecular tool in a wide array of applications—ranging from blotting, immunoprecipitation, and ELISA development, to probe design and fluorescent signal enhancement. The biotin modification facilitates strong, specific interactions with streptavidin derivatives for immobilization, pull-down, or detection systems. Its compatibility with storage at 2–8°C in dry form aids in long-term use and shipping stability. Researchers can rely on this reagent to integrate seamlessly into existing GFP-based workflows or to create novel assay formats without the need for custom conjugation.

Because GFP is a common reporter tag in recombinant proteins, this antibody provides predictable, high-affinity recognition. Its biotechnology-grade production ensures repeatability, while the easy-to-handle lyophilized format supports precise formulation for small- or large-scale assays. Overall, this biotinylated chicken anti-GFP antibody is a practical choice for labs requiring dependable, high-purity performance in affinity capture, detection, or labeling studies.

Why researchers choose this product:

• 100 μg of biotin-labeled anti-GFP antibody in a stable powder format
• High purity, biotech-grade quality from ultrapure water and 0.22 µm filtration
• Lyophilized form facilitates flexible reconstitution and long shelf stability
• Biotin tag enables strong and specific binding to streptavidin for capture/detection
• Ideal for use in GFP detection workflows including IP, blotting, ELISA, and labeling

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

• 1: Kortus S, Rehakova K, Klima M, Kolcheva M, Ladislav M, Langore E, Barackova P, Netolicky J, Misiachna A, Hemelikova K, Humpolickova J, Chalupska D, Silhan J, Kaniakova M, Hrcka Krausova B, Boura E, Zapotocky M, Horak M. Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes J Neurosci. 2023 Jun 28;43(26):4755-4774.
• 2: Altalhi W, Wu T, Wojtkiewicz GR, Jeffs S, Miki K, Ott HC. Intratracheally injected human-induced pluripotent stem cell-derived pneumocytes and endothelial cells engraft in the distal lung and ameliorate emphysema in a rat model J Thorac Cardiovasc Surg. 2023 Jul;166(1):e23-e37.
• 3: Jacková B, Mottet G, Rudiuk S, Morel M, Baigl D. DNA-Encoded Immunoassay in Picoliter Drops: A Minimal Cell-Free Approach Adv Biol (Weinh). 2023 Mar;7(3):e2200266.
• 4: Chao S, Liu Y, Ding N, Lin Y, Wang Q, Tan J, Li W, Zheng Y, Hu X, Li J. Highly Expressed Soluble Recombinant Anti-GFP VHHs in Escherichia coli via Optimized Signal Peptides, Strains, and Inducers Front Mol Biosci. 2022 Mar 10;9:848829.
• 5: Yilmaz Z, Jedlitzke B, Mootz HD. Design and Preparation of Photobodies: Light-Activated Single-Domain Antibody Fragments Methods Mol Biol. 2022;2446:409-424.
• 6: Matsuda S, Aguilar G, Vigano MA, Affolter M. Nanobody-Based GFP Traps to Study Protein Localization and Function in Developmental Biology Methods Mol Biol. 2022;2446:581-593.
• 7: Philippi M, Richter CP, Kappen M, Watrinet I, Miao Y, Runge M, Jorde L, Korneev S, Holtmannspötter M, Kurre R, Holthuis JCM, Garcia KC, Plückthun A, Steinhart M, Piehler J, You C. Biofunctional Nanodot Arrays in Living Cells Uncover Synergistic Co-Condensation of Wnt Signalodroplets Small. 2022 Dec;18(50):e2203723.
• 8: Boncompain G, Fourriere L, Gareil N, Perez F. Retention Using Selective Hooks-Synchronized Secretion to Measure Local Exocytosis Methods Mol Biol. 2021;2233:253-264.
• 9: Marsango S, Jenkins L, Pediani JD, Bradley SJ, Ward RJ, Hesse S, Biener G, Stoneman MR, Tobin AB, Raicu V, Milligan G. The M(1) muscarinic receptor is present in situ as a ligand-regulated mixture of monomers and oligomeric complexes Proc Natl Acad Sci U S A. 2022 Jun 14;119(24):e2201103119.
• 10: Latif R, Michalek K, Davies TF. Subunit interactions influence TSHR multimerization Mol Endocrinol. 2010 Oct;24(10):2009-18. pubmed.ncbi.nlm.nih.gov