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Biotinylated Mononucleosomes (H1.2) Proteins Molecular Depot
Biotinylated Mononucleosomes (H1.2) Proteins Molecular Depot
Biotinylated Mononucleosomes (H1.2) Proteins Molecular Depot
Biotinylated Mononucleosomes (H1.2) Proteins Molecular Depot

Biotinylated Mononucleosomes (H1.2)

$2,195.00

    Catalog Number: B2016651 (10 µg)

    Biotinylated Mononucleosomes (H1.2) is are high quality specialized protein-DNA complexes used in various research fields to study chromatin structure, gene regulation, and epigenetic modifications. These complexes consist of a segment of DNA wrapped around histone proteins, with the addition of biotin molecules for specific labeling and detection purposes. Researchers can use these biotinylated mononucleosomes to investigate how proteins interact with chromatin, study the effects of histone modifications, and explore the mechanisms of gene expression and silencing. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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What's Included

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VR Headset

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2 Touch Controllers

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Charging Cable

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Power Adapter

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Biotinylated Mononucleosomes (H1.2)

Catalog number: B2016651
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 10 µg
Molecular Weight or Concentration: histone octamer and histone H1.2 are 108 kDa and 21.4 kDa, respectively
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -80°C
Keywords: Recombinant Mononucleosomes (H1.2) – Biotin
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References

  • Gerodimos CA, Watanabe G, Lieber MR. Nonhomologous DNA end joining of nucleosomal substrates in a purified system DNA Repair (Amst). 2021 Oct;106:103193.
  • Laflamme L, Guillemette B, Gaudreau L. An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro Methods Mol Biol. 2017;1528:19-37.
  • Fang H, Wei S, Lee TH, Hayes JJ. Chromatin structure-dependent conformations of the H1 CTD Nucleic Acids Res. 2016 Nov 2;44(19):9131-9141.
  • Changolkar LN, Pehrson JR. Reconstitution of nucleosomes with histone macroH2A1.2 Biochemistry. 2002 Jan 8;41(1):179-84.
  • Aizawa M, Sugimoto N, Watanabe S, Yoshida K, Fujita M. Nucleosome assembly and disassembly activity of GRWD1, a novel Cdt1-binding protein that promotes pre-replication complex formation Biochim Biophys Acta. 2016 Nov;1863(11):2739-2748.
  • Kepert JF, Tóth KF, Caudron M, Mücke N, Langowski J, Rippe K. Conformation of reconstituted mononucleosomes and effect of linker histone H1 binding studied by scanning force microscopy Biophys J. 2003 Dec;85(6):4012-22.
  • Bennett L, Madders ECET, Parsons JL. HECTD1 promotes base excision repair in nucleosomes through chromatin remodelling Nucleic Acids Res. 2020 Feb 20;48(3):1301-1313.
  • Le DD, Cortesi AT, Myers SA, Burlingame AL, Fujimori DG. Site-specific and regiospecific installation of methylarginine analogues into recombinant histones and insights into effector protein binding J Am Chem Soc. 2013 Feb 27;135(8):2879-82.
  • Ho KK, Zhang H, Golden BL, Ogas J. PICKLE is a CHD subfamily II ATP-dependent chromatin remodeling factor Biochim Biophys Acta. 2013 Feb;1829(2):199-210.

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    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

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    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

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Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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