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Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO Real-Time PCR Detection Kits Biomiga
by Biomiga

Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO

Pack Size: 1000 Tests
Authorized Dealer
20 pathogens
Bacteria, E. coli types, parasites, viruses
7 mixes
Multiplex reactions per sample
200 copies/mL
Stated analytical sensitivity
4 channels
FAM, JOE/HEX/VIC, ROX, Cy5

ONE STOOL EXTRACT, FOUR PATHOGEN CLASSES

A full gastrointestinal pathogen menu on your existing real-time PCR instrument.

The Biomiga GI Panel Real-Time PCR Detection Kit (QP2166) is a qualitative multiplex real-time PCR assay for bacterial, diarrheagenic E. coli, parasitic and viral nucleic acids in unpreserved stool collected in Cary-Blair transport medium. Targets run across seven multiplex mixes, each read in four fluorescence channels, with a human RNase P internal control and kit positive and negative controls. Built-in marker logic separates E. coli pathotypes, including STEC, EHEC, EPEC, EAEC, ETEC and EIEC/Shigella.

For research use only. Not for use in diagnostic procedures.

Biomiga GI Panel QP2166 workflow: stool sample in Cary-Blair, extract, 7 multiplex mixes, 20 pathogens called

Target menu

BACTERIA
  • Campylobacter (C. jejuni / C. coli)
  • Salmonella
  • Yersinia enterocolitica
  • Vibrio parahaemolyticus
  • Vibrio vulnificus
DIARRHEAGENIC E. COLI / SHIGELLA
  • Enteroaggregative E. coli (EAEC)
  • Enteropathogenic E. coli (EPEC)
  • Shigella / enteroinvasive E. coli (EIEC)
  • Shiga-like toxin-producing E. coli (STEC)
  • Enterotoxigenic E. coli (ETEC)
  • Enterohemorrhagic E. coli (EHEC)
PARASITES
  • Cryptosporidium
  • Cyclospora cayetanensis
  • Entamoeba histolytica
  • Giardia lamblia
VIRUSES
  • Adenovirus F40/41
  • Astrovirus
  • Norovirus GI / GII
  • Rotavirus A
  • Sapovirus (I, II, IV and V)

Target list per Biomiga's instructions for use (IFU), March 2026. The mix layout also includes a C. difficile toxin B (tcdB) marker and an E. coli O157 marker.

Biomiga GI Panel QP2166 testing menu: 5 bacteria, 6 E. coli types, 4 parasites, 5 viruses

Mix layout

Mix JOE FAM ROX Cy5
1 Astrovirus Adenovirus Rotavirus Norovirus GI
2 tcdB Norovirus GII aaiC Sapovirus
3 Y. enterocolitica V. parahaemolyticus Cryptosporidium C. cayetanensis
4 Campylobacter Salmonella G. lamblia RNase P (control)
5 eae stx2 aggR ipaH
6 stx1 uidA V. vulnificus E. histolytica
7 O157 (VIC) STa STb LT

How the E. coli calls work

The kit identifies E. coli with the uidA marker, then assigns a pathotype from the virulence genes detected alongside it:

EIEC / Shigella

uidA + ipaH

EAEC

uidA + aggR or aaiC, no stx

EPEC

uidA + eae, no stx

EHEC

uidA + eae + stx1 and/or stx2

STEC

uidA + stx1 and/or stx2, no eae or aggR/aaiC

EAggSTEC

uidA + aggR or aaiC + stx1 and/or stx2

ETEC

uidA + STa, STb or LT

Summarized from the E. coli interpretation table in Biomiga's IFU.

How it works

STEP 1

Extract

Purify DNA/RNA from stool in Cary-Blair medium with an extraction kit (not included).

STEP 2

Set up 7 mixes

15 µL of each GI PCR Mix plus 5 µL of extract or control, for a 20 µL reaction.

STEP 3

Run

50 °C for 5 min, 95 °C for 2 min, then 40 cycles of 95 °C for 5 s and 60 °C for 20 s.

STEP 4

Read

Confirm the controls pass, then call each target by channel and assign E. coli pathotypes from the marker table.

INSTRUMENTS

Runs on the cyclers labs already own

Biomiga lists the Applied Biosystems 7500, Thermo Fisher QuantStudio 5, 7 and 12K, and Bio-Rad CFX96 and CFX384 as compatible. Any real-time cycler with FAM, JOE (HEX or VIC), ROX and Cy5 channels meets the IFU's requirement.

You supply: DNA/RNA extraction reagents, Cary-Blair collection containers, PCR tubes or plates, RNase-free 1.5 mL tubes, a vortex, a high-speed centrifuge, micropipettes with filter tips, and PPE.

What's in the box

Component 100 tests 1,000 tests
GI PCR Mix 1–7 1.5 mL each 15 mL each
Positive Control 100 µL 500 µL
Negative Control 100 µL 500 µL

Built for enteric pathogen research

Surveillance studies

Profile bacterial, parasitic and viral targets across stool sample sets.

E. coli pathotyping

Separate STEC, EHEC, EPEC, EAEC, ETEC and EIEC by virulence markers.

Food and water safety research

Salmonella, Campylobacter, Vibrio, norovirus and more in one workflow.

Assay development

A broad multiplex reference for method comparison and validation.

When this kit is the right call

  • No new analyzer: runs on ABI, QuantStudio and Bio-Rad CFX instruments.
  • Four pathogen classes: bacteria, E. coli pathotypes, parasites and viruses from one extract.
  • Built-in E. coli logic: pathotype calls from marker combinations, not a single target.
  • Scales up: a 1,000-test kit for high-throughput labs.

Related Biomiga kits

THIS KIT

GI Panel (QP2166)

20 enteric pathogens in 7 mixes.

Respiratory Panel (QP2179)

11 respiratory viruses in 3 reactions.

Pneumonia Panel (QP2168)

13 bacterial targets in 4 panels.

MgPure Universal Pathogen DNA/RNA Purification Kit (MR6532.CX.A96)

Biomiga's magnetic-bead extraction for the first step.

Need one of the others? Email info@bluetigerscientific.com or text (833) 928-4437 for a quote.

Frequently asked questions

Quick facts

What it is: A multiplex real-time PCR reagent kit for 20 enteric bacteria, E. coli pathotypes, parasites and viruses, in seven mixes per sample.

Sample type: Nucleic acid extracted from unpreserved stool in Cary-Blair transport medium.

Instruments: ABI 7500; QuantStudio 5, 7, 12K; Bio-Rad CFX96, CFX384.

Pack sizes: 100 tests and 1,000 tests.

Use: Research use only. Not for use in diagnostic procedures.

Which pathogens does the panel detect?

Bacteria: Campylobacter (C. jejuni/C. coli), Salmonella, Yersinia enterocolitica, Vibrio parahaemolyticus, Vibrio vulnificus. E. coli/Shigella: EAEC, EPEC, Shigella/EIEC, STEC, ETEC, EHEC. Parasites: Cryptosporidium, Cyclospora cayetanensis, Entamoeba histolytica, Giardia lamblia. Viruses: adenovirus F40/41, astrovirus, norovirus GI/GII, rotavirus A, sapovirus (I, II, IV, V).

Can this kit be used for patient diagnosis?

No. In the United States it is supplied for research use only and is not for use in diagnostic procedures.

What sample type is validated?

Unpreserved stool collected in Cary-Blair transport medium. Biomiga advises processing samples promptly, or holding them at 4 °C for up to 24 hours or at -80 °C for longer storage.

Is an extraction kit included?

No. Nucleic acid must be extracted before testing. Biomiga's MgPure Universal Pathogen DNA/RNA Purification Kit (MR6532.CX.A96) is one option; ask us for a quote.

Will it run on my instrument?

Biomiga lists the ABI 7500, QuantStudio 5, 7 and 12K, and Bio-Rad CFX96 and CFX384. Other cyclers need FAM, JOE (HEX/VIC), ROX and Cy5 channels; contact us before ordering if you run a different platform.

How sensitive is the assay?

Biomiga states an analytical sensitivity of 200 copies/mL.

How is the kit shipped and stored?

It ships frozen. Store at -20 ± 5 °C and keep freeze-thaw cycles under five. Biomiga states 12 months of validity, with the expiry date printed on the label.

Can my institution get a formal quote?

Yes. Email info@bluetigerscientific.com or text (833) 928-4437 for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.

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Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO Real-Time PCR Detection Kits Biomiga
Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO Real-Time PCR Detection Kits Biomiga
Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO Real-Time PCR Detection Kits Biomiga
Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO Real-Time PCR Detection Kits Biomiga
Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO Real-Time PCR Detection Kits Biomiga
Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO Real-Time PCR Detection Kits Biomiga
Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO Real-Time PCR Detection Kits Biomiga
Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO Real-Time PCR Detection Kits Biomiga
Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO Real-Time PCR Detection Kits Biomiga
Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO Real-Time PCR Detection Kits Biomiga

Biomiga GI Panel Real-Time PCR Detection Kit, 20 Pathogens (QP2166), RUO

$66,666.67

    Multiplex real-time PCR reagent kit for 20 gastrointestinal pathogens, covering bacteria, E. coli pathotypes, parasites and viruses, from stool in Cary-Blair medium.

    • Salmonella, Campylobacter, Yersinia, Vibrio, Cryptosporidium, Giardia, norovirus, rotavirus and more
    • Built-in marker logic for STEC, EHEC, EPEC, EAEC, ETEC and EIEC/Shigella
    • Seven multiplex mixes; stated analytical sensitivity of 200 copies/mL; RNase P internal control
    • Runs on ABI 7500, QuantStudio 5/7/12K and Bio-Rad CFX96/384

    Products are for in vitro research use only (RUO). Not for use in diagnostic procedures.

    Live Inquiry about this product via:

    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

See All Specs

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Fast Delivery

Most items ship same day

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Manufacturer Warranty

All products are covered by manufacturer warranty

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After-Sale Support

24/7 support

GET A QUOTE
20 pathogens
Bacteria, E. coli types, parasites, viruses
7 mixes
Multiplex reactions per sample
200 copies/mL
Stated analytical sensitivity
4 channels
FAM, JOE/HEX/VIC, ROX, Cy5

ONE STOOL EXTRACT, FOUR PATHOGEN CLASSES

A full gastrointestinal pathogen menu on your existing real-time PCR instrument.

The Biomiga GI Panel Real-Time PCR Detection Kit (QP2166) is a qualitative multiplex real-time PCR assay for bacterial, diarrheagenic E. coli, parasitic and viral nucleic acids in unpreserved stool collected in Cary-Blair transport medium. Targets run across seven multiplex mixes, each read in four fluorescence channels, with a human RNase P internal control and kit positive and negative controls. Built-in marker logic separates E. coli pathotypes, including STEC, EHEC, EPEC, EAEC, ETEC and EIEC/Shigella.

For research use only. Not for use in diagnostic procedures.

Biomiga GI Panel QP2166 workflow: stool sample in Cary-Blair, extract, 7 multiplex mixes, 20 pathogens called

Target menu

BACTERIA
  • Campylobacter (C. jejuni / C. coli)
  • Salmonella
  • Yersinia enterocolitica
  • Vibrio parahaemolyticus
  • Vibrio vulnificus
DIARRHEAGENIC E. COLI / SHIGELLA
  • Enteroaggregative E. coli (EAEC)
  • Enteropathogenic E. coli (EPEC)
  • Shigella / enteroinvasive E. coli (EIEC)
  • Shiga-like toxin-producing E. coli (STEC)
  • Enterotoxigenic E. coli (ETEC)
  • Enterohemorrhagic E. coli (EHEC)
PARASITES
  • Cryptosporidium
  • Cyclospora cayetanensis
  • Entamoeba histolytica
  • Giardia lamblia
VIRUSES
  • Adenovirus F40/41
  • Astrovirus
  • Norovirus GI / GII
  • Rotavirus A
  • Sapovirus (I, II, IV and V)

Target list per Biomiga's instructions for use (IFU), March 2026. The mix layout also includes a C. difficile toxin B (tcdB) marker and an E. coli O157 marker.

Biomiga GI Panel QP2166 testing menu: 5 bacteria, 6 E. coli types, 4 parasites, 5 viruses

Mix layout

Mix JOE FAM ROX Cy5
1 Astrovirus Adenovirus Rotavirus Norovirus GI
2 tcdB Norovirus GII aaiC Sapovirus
3 Y. enterocolitica V. parahaemolyticus Cryptosporidium C. cayetanensis
4 Campylobacter Salmonella G. lamblia RNase P (control)
5 eae stx2 aggR ipaH
6 stx1 uidA V. vulnificus E. histolytica
7 O157 (VIC) STa STb LT

How the E. coli calls work

The kit identifies E. coli with the uidA marker, then assigns a pathotype from the virulence genes detected alongside it:

EIEC / Shigella

uidA + ipaH

EAEC

uidA + aggR or aaiC, no stx

EPEC

uidA + eae, no stx

EHEC

uidA + eae + stx1 and/or stx2

STEC

uidA + stx1 and/or stx2, no eae or aggR/aaiC

EAggSTEC

uidA + aggR or aaiC + stx1 and/or stx2

ETEC

uidA + STa, STb or LT

Summarized from the E. coli interpretation table in Biomiga's IFU.

How it works

STEP 1

Extract

Purify DNA/RNA from stool in Cary-Blair medium with an extraction kit (not included).

STEP 2

Set up 7 mixes

15 µL of each GI PCR Mix plus 5 µL of extract or control, for a 20 µL reaction.

STEP 3

Run

50 °C for 5 min, 95 °C for 2 min, then 40 cycles of 95 °C for 5 s and 60 °C for 20 s.

STEP 4

Read

Confirm the controls pass, then call each target by channel and assign E. coli pathotypes from the marker table.

INSTRUMENTS

Runs on the cyclers labs already own

Biomiga lists the Applied Biosystems 7500, Thermo Fisher QuantStudio 5, 7 and 12K, and Bio-Rad CFX96 and CFX384 as compatible. Any real-time cycler with FAM, JOE (HEX or VIC), ROX and Cy5 channels meets the IFU's requirement.

You supply: DNA/RNA extraction reagents, Cary-Blair collection containers, PCR tubes or plates, RNase-free 1.5 mL tubes, a vortex, a high-speed centrifuge, micropipettes with filter tips, and PPE.

What's in the box

Component 100 tests 1,000 tests
GI PCR Mix 1–7 1.5 mL each 15 mL each
Positive Control 100 µL 500 µL
Negative Control 100 µL 500 µL

Built for enteric pathogen research

Surveillance studies

Profile bacterial, parasitic and viral targets across stool sample sets.

E. coli pathotyping

Separate STEC, EHEC, EPEC, EAEC, ETEC and EIEC by virulence markers.

Food and water safety research

Salmonella, Campylobacter, Vibrio, norovirus and more in one workflow.

Assay development

A broad multiplex reference for method comparison and validation.

When this kit is the right call

  • No new analyzer: runs on ABI, QuantStudio and Bio-Rad CFX instruments.
  • Four pathogen classes: bacteria, E. coli pathotypes, parasites and viruses from one extract.
  • Built-in E. coli logic: pathotype calls from marker combinations, not a single target.
  • Scales up: a 1,000-test kit for high-throughput labs.

Related Biomiga kits

THIS KIT

GI Panel (QP2166)

20 enteric pathogens in 7 mixes.

Respiratory Panel (QP2179)

11 respiratory viruses in 3 reactions.

Pneumonia Panel (QP2168)

13 bacterial targets in 4 panels.

MgPure Universal Pathogen DNA/RNA Purification Kit (MR6532.CX.A96)

Biomiga's magnetic-bead extraction for the first step.

Need one of the others? Email info@bluetigerscientific.com or text (833) 928-4437 for a quote.

Frequently asked questions

Quick facts

What it is: A multiplex real-time PCR reagent kit for 20 enteric bacteria, E. coli pathotypes, parasites and viruses, in seven mixes per sample.

Sample type: Nucleic acid extracted from unpreserved stool in Cary-Blair transport medium.

Instruments: ABI 7500; QuantStudio 5, 7, 12K; Bio-Rad CFX96, CFX384.

Pack sizes: 100 tests and 1,000 tests.

Use: Research use only. Not for use in diagnostic procedures.

Which pathogens does the panel detect?

Bacteria: Campylobacter (C. jejuni/C. coli), Salmonella, Yersinia enterocolitica, Vibrio parahaemolyticus, Vibrio vulnificus. E. coli/Shigella: EAEC, EPEC, Shigella/EIEC, STEC, ETEC, EHEC. Parasites: Cryptosporidium, Cyclospora cayetanensis, Entamoeba histolytica, Giardia lamblia. Viruses: adenovirus F40/41, astrovirus, norovirus GI/GII, rotavirus A, sapovirus (I, II, IV, V).

Can this kit be used for patient diagnosis?

No. In the United States it is supplied for research use only and is not for use in diagnostic procedures.

What sample type is validated?

Unpreserved stool collected in Cary-Blair transport medium. Biomiga advises processing samples promptly, or holding them at 4 °C for up to 24 hours or at -80 °C for longer storage.

Is an extraction kit included?

No. Nucleic acid must be extracted before testing. Biomiga's MgPure Universal Pathogen DNA/RNA Purification Kit (MR6532.CX.A96) is one option; ask us for a quote.

Will it run on my instrument?

Biomiga lists the ABI 7500, QuantStudio 5, 7 and 12K, and Bio-Rad CFX96 and CFX384. Other cyclers need FAM, JOE (HEX/VIC), ROX and Cy5 channels; contact us before ordering if you run a different platform.

How sensitive is the assay?

Biomiga states an analytical sensitivity of 200 copies/mL.

How is the kit shipped and stored?

It ships frozen. Store at -20 ± 5 °C and keep freeze-thaw cycles under five. Biomiga states 12 months of validity, with the expiry date printed on the label.

Can my institution get a formal quote?

Yes. Email info@bluetigerscientific.com or text (833) 928-4437 for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.

    SpecificationDetail
    Catalog NumberQP2166 (100 tests, 1,000 tests)
    MethodMultiplex real-time PCR / RT-PCR, probe-based, qualitative
    Targets20 enteric pathogens: 5 bacteria, 6 diarrheagenic E. coli/Shigella types, 4 parasites, 5 viruses
    Internal ControlHuman RNase P (Mix 4)
    Reactions per Sample7 (GI PCR Mix 1–7)
    Reaction Volume20 µL (15 µL mix + 5 µL extract)
    Detection ChannelsFAM, JOE (HEX/VIC), ROX, Cy5
    Cycling50 °C 5 min; 95 °C 2 min; 40 x (95 °C 5 s, 60 °C 20 s)
    Analytical Sensitivity200 copies/mL (as stated by Biomiga)
    Sample TypeExtracted nucleic acid from unpreserved stool in Cary-Blair transport medium
    Listed InstrumentsABI 7500; QuantStudio 5, 7, 12K; Bio-Rad CFX96, CFX384
    Shipping and StorageShips frozen. Store at -20 ± 5 °C; fewer than 5 freeze-thaw cycles
    Shelf Life12 months; expiry printed on the label
    Country of OriginUSA
    Intended UseResearch use only (RUO). Not for use in diagnostic procedures

    Terms & Conditions

    Blue Tiger Scientific is an independent distributor of products manufactured by Biomiga, Inc. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms.

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnostic procedures, or therapy, and may not be resold to consumers. Sales are limited to verified institutional and business customers shipping to a business address; we do not ship to residential addresses. Blue Tiger Scientific may request institutional verification or end-use information before shipping and may cancel any order that cannot be verified.

    Payment Terms
    Orders are prepaid by card, or invoiced against an institutional purchase order once credit is approved. Prices are in USD and subject to change without notice. Purchase orders are due within 30 days of invoice unless agreed otherwise in writing.

    Order Confirmation & Cancellations
    Confirmed orders are placed with the manufacturer and are final. Changes or cancellations must be requested in writing before shipment and approved by Blue Tiger Scientific; approved cancellations may incur restocking, materials or third-party fees.

    Accuracy of Orders
    Customers are responsible for verifying catalog numbers, pack sizes, ship-to address and recipient availability before submitting an order.

    Shipping & Cold Chain
    Shipping is calculated at checkout. Temperature-sensitive products ship by expedited service, and ship dates may be adjusted to avoid weekend or holiday transit. Orders placed on weekends or holidays are processed the next business day. Delivery dates are estimates and are not guaranteed. A recipient must be available to receive and store temperature-sensitive products on arrival. Blue Tiger Scientific is not liable for carrier delays, weather disruptions or other events beyond our control.

    Receiving & Claims
    Inspect every shipment on arrival. Shortages, damage or thawed temperature-sensitive product must be reported to Blue Tiger Scientific in writing, with photos, within 2 business days of delivery. Claims received after that window, and shipments that are refused, unclaimed or left unattended, are not eligible for credit or replacement.

    Returns & Refunds
    Returns require a Return Merchandise Authorization (RMA) issued by Blue Tiger Scientific and must be requested within 30 days of delivery. Refunds or replacements are issued only for shipping errors or verified product defects. Temperature-sensitive products are not returnable once delivered. Customers pay return shipping unless the error was ours, and refunds exclude original shipping charges. Approved refunds are processed within 45 business days.

    Warranty
    Biomiga warrants its products to perform as described in their labeling when used in accordance with their instructions; the remedy for a product that does not is replacement, at Biomiga's option. Blue Tiger Scientific coordinates these claims with Biomiga. No other warranties, express or implied, are given.

    Limitation of Liability
    Blue Tiger Scientific's total liability for any claim is limited to the purchase price of the product involved. Blue Tiger Scientific and Biomiga are not liable for indirect, incidental or consequential damages, including lost research time or materials, arising from the use or misuse of any product.

    Safety
    Handle all products according to the Safety Data Sheet and the manufacturer's instructions, using appropriate personal protective equipment.

    Legal & Export Compliance
    Customers must use products in compliance with all applicable local, state, federal and international laws, including export control and biosafety regulations. Products may not be exported or re-exported without the required authorizations.

    Resale
    Products may not be resold or redistributed without prior written approval from Blue Tiger Scientific.

    Force Majeure
    Neither party is liable for delays or failures caused by events beyond its reasonable control, including weather, carrier disruptions, supply shortages or changes in regulation.

    Governing Law
    These terms are governed by the laws of the State of California and the United States.

$66,666.67 USD 1000 Tests

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