AGO1 Protein – Catalog number: B2016695 (10 µg)

AGO1 Protein (Argonaute 1) is provided as a biotechnology-grade solution in a defined 10 µg amount (Catalog B2016695) with a listed molecular weight of 100.2 kDa, and storage specified at −80 °C. AGO1 belongs to the Argonaute protein family at the core of the RNA-induced silencing complex (RISC), which loads small RNAs (e.g., microRNAs) to guide sequence-dependent interactions with messenger RNAs. In typical models of miRNA-mediated regulation, AGO1 participates in target recognition that can lead to translational repression and mRNA decay through recruitment of effector factors. In line with the supplier’s listing, this material is positioned as a molecular tool for various biochemical applications, supporting exploratory work where a defined Argonaute component is required.

For method development, a clearly specified AGO1 preparation enables construction of proof-of-concept RISC assemblies, pilot binding or pull-down workflows using small-RNA guides, and qualitative reference experiments that benchmark buffer components, loading conditions, and background behavior. Because the listing does not declare a working concentration, teams typically establish conditions empirically: start with small-scale checkerboard titrations, document diluent composition and time-at-temperature, and retain lot identifiers to ensure reproducibility across runs and operators. The solution format minimizes variability associated with reconstitution, allowing direct dilution into user-selected buffers. The biotechnology-grade note (solutions prepared with Type I ultrapure water and filtered through 0.22 µm) aligns with expectations for low background during bench-scale optimization.

Common practical considerations include preparing aliquots to avoid repeated freeze–thaw cycles, confirming guide-RNA integrity during assembly steps, and recording any co-factors or salts added during method setup. With its concise, traceable specification set (amount, molecular weight, storage, and recognized synonyms), AGO1 Protein integrates cleanly into SOPs, inventory systems, and training or platform-qualification runs that examine Argonaute-dependent mechanisms in research contexts.

Why researchers choose this product:

• Defined 10 µg fill with listed 100.2 kDa molecular weight for precise documentation
• Ready-to-dilute solution format streamlines pilot titrations and buffer screening
• Specified storage at −80 °C supports long-term stability planning
• Biotechnology-grade preparation (Type I water; 0.22 µm filtration for solutions) to reduce background
• Extensive synonym list (e.g., Argonaute1, EIF2C1) aids LIMS/SOP searchability

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

• 1: Ghosh S, Liu JL. Genomic Tagging of AGO1 Using CRISPR/Cas9-Mediated Homologous Recombination Methods Mol Biol. 2018;1680:217-235.
• 2: Stoica C, Carmichael JB, Parker H, Pare J, Hobman TC. Interactions between the RNA interference effector protein Ago1 and 14-3-3 proteins: consequences for cell cycle progression J Biol Chem. 2006 Dec 8;281(49):37646-51.
• 3: Zofall M, Grewal SI. RNAi-mediated heterochromatin assembly in fission yeast Cold Spring Harb Symp Quant Biol. 2006;71:487-96.
• 4: Park MS, Phan HD, Busch F, Hinckley SH, Brackbill JA, Wysocki VH, Nakanishi K. Human Argonaute3 has slicer activity Nucleic Acids Res. 2017 Nov 16;45(20):11867-11877.
• 5: Xu D, Yang H, Zou C, Li WX, Xu Y, Xie C. Identification and functional characterization of the AGO1 ortholog in maize J Integr Plant Biol. 2016 Aug;58(8):749-58.
• 6: Friend K, Campbell ZT, Cooke A, Kroll-Conner P, Wickens MP, Kimble J. A conserved PUF-Ago-eEF1A complex attenuates translation elongation Nat Struct Mol Biol. 2012 Jan 8;19(2):176-83.
• 7: Elkayam E, Faehnle CR, Morales M, Sun J, Li H, Joshua-Tor L. Multivalent Recruitment of Human Argonaute by GW182 Mol Cell. 2017 Aug 17;67(4):646-658.e3.
• 8: Bellissimo T, Tito C, Ganci F, Sacconi A, Masciarelli S, Di Martino G, Porta N, Cirenza M, Sorci M, De Angelis L, Rosa P, Calogero A, Fatica A, Petrozza V, Fontemaggi G, Blandino G, Fazi F. Argonaute 2 drives miR-145-5p-dependent gene expression program in breast cancer cells Cell Death Dis. 2019 Jan 8;10(1):17.
• 9: Yoshikawa M, Han YW, Fujii H, Aizawa S, Nishino T, Ishikawa M. Cooperative recruitment of RDR6 by SGS3 and SDE5 during small interfering RNA amplification in Arabidopsis Proc Natl Acad Sci U S A. 2021 Aug 24;118(34):e2102885118.
• 10: Dunoyer P, Schott G, Himber C, Meyer D, Takeda A, Carrington JC, Voinnet O. RETRACTED: Small RNA duplexes function as mobile silencing signals between plant cells Science. 2010 May 14;328(5980):912-6.