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qMAX™ Probe One-Step RT-qPCR Kits Accuris
qMAX™ Green One-Step RT-qPCR Kits Accuris
qMAX™ Green One-Step RT-qPCR Kits Accuris
qMAX™ Green One-Step RT-qPCR Kits Accuris
qMAX™ Probe One-Step RT-qPCR Kits Accuris
qMAX™ Green One-Step RT-qPCR Kits Accuris
qMAX™ Green One-Step RT-qPCR Kits Accuris
qMAX™ Green One-Step RT-qPCR Kits Accuris

qMAX™ Probe One-Step RT-qPCR Kits

$20.25

    RNA to cDNA to qPCR, in one tube

    Simplify your workflows with Accuris qMAX™ One Step Kits. These innovative kits provide all the necessary components for direct RT-qPCR from RNA templates, eliminating the need for separate reverse transcription and qPCR steps. With optimized formulations and high sensitivity, Accuris qMAX™ Kits deliver reliable and reproducible results for a wide range of applications.

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Accuris qMAX™ One Step Kits enable highly sensitive real-time PCR RT-qPCR assays directly from RNA templates, simplifying workflows with their ready-to-use 2X qPCR master mix and 20X reverse transcriptase formulations.

The optimized buffer contains powerful RNase inhibitors and a highly thermostable MMLV-derived reverse transcriptase for robust first-strand cDNA synthesis. The Accuris Hot Start Taq employs an antibody-mediated hot start mechanism, allowing sample preparation at room temperature and becoming active only after an initial incubation at 95°C, thus minimizing non-specific amplification. An inert blue dye in the Taq master mix aids in simplifying pipetting and reducing errors during real-time PCR.

Starting Material: RNA

  • Two-tube formulation: 2X Probe One-Step Mix & 20X RTase Blend
    (Includes Hot Start Taq Polymerase)
  • Convenient cDNA synthesis and subsequent qPCR in a single tube
  • Ideal for gene expression analysis & single pathogen detection


Each 100 reaction kit includes:

  • 1 tube of 20X thermostable MMLV derived RTase (RNase inhibitor included)
  • 1 tube of 2X Probe One-Step Mix
  • Includes Accuris Hot Start Taq DNA Polymerase (dNTPs)
  • Optimized buffer for maximum efficiency & sensitivity

Applications:

  • Single Pathogen Detection
  • Gene Expression Analysis

qMAX™ kits are available in three versions:

  • qMAX Green One Step kits: Utilize a proprietary intercalating dye with higher fluorescence and lower PCR inhibition compared to popular dyes like SYBR.

  • qMAX Probe One Step kits: Optimized for use with TaqMan, Scorpions, and molecular beacon probes.

  • qMAX Probe One Step Multiplex kits: Designed for efficient probe-based detection of multiple targets in a single reaction well, ideal for complex RNA samples, including low-copy number viral RNA during real-time PCR. These kits balance critical factors such as magnesium chloride and deoxynucleotide concentrations, Taq Polymerase and reverse transcriptase concentrations, and the ionic conditions of the reaction buffer.

All Accuris One-Step Kits support standard and fast cycling protocols, offering increased sensitivity, speed, and reproducibility for a wide range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments for real-time PCR.

Bulk Packaging Available:

For high-throughput users, Accuris now offers their complete line of qPCR and RT-qPCR reagents in bulk packaging at discounted prices. Bottle sizes of 25ml (2500 reactions) and 50ml (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk for 2500 and 5000 reactions, ideal for labs performing pathogen detection.

Key Features:

  • RNA to cDNA to qPCR in a single tube
  • High-purity enzyme formulation for enhanced stability and performance
  • Accuris Hot-Start Taq allows for room-temperature preparation
  • Blue dye for easy pipetting and visualization in plates
  • Available for green fluorescence or probe detection
  • Multiplex formulation for multiple target amplification

    Accuris qMAX Green One Step qPCR Kit

    Feature Details
    Components 2X qPCR Master Mix
    20X Reverse Transcriptase
    Inert Blue Dye for pipetting and visualization
    RNase Inhibitors
    Accuris Hot Start Taq DNA Polymerase
    Key Features High Sensitivity: Capable of detecting low levels of RNA.
    Room Temperature Preparation: Sample preparation can be done at room temperature.
    Reduced Non-Specific Amplification: Initial incubation at 95°C activates the Taq enzyme, reducing non-specific amplification.
    Intercalating Dye: Proprietary dye with higher fluorescence and lower PCR inhibition compared to SYBR.
    Broad Compatibility: Compatible with standard and fast cycling protocols.
    Bulk Packaging: Available in 25ml (2500 reactions) and 50ml (5000 reactions) bottle sizes.
    Usage RNA Input: 10pg to 100ng total RNA per reaction (or a minimum of 0.01pg mRNA per reaction).
    Reverse Transcription: Recommended incubation at 50°C for 10 minutes.
    PCR Cycling Conditions: Initial denaturation at 95°C for 2 minutes, followed by 30-40 cycles of 95°C for 5 seconds (denaturation) and 60-65°C for 20-30 seconds (annealing/extension).
    Applications Gene expression analysis & Fluorescent Detection of DNA/cDNA
    Quantification of specific target RNA from total RNA or mRNA.
    Suitable for research purposes only, not intended for therapeutic or diagnostic use.

    Accuris qMAX Probe One Step qPCR Kit

    Feature Details
    Components 2X qPCR Master Mix
    20X Reverse Transcriptase
    RNase Inhibitors
    Accuris Hot Start Taq DNA Polymerase
    Inert Blue Dye for pipetting and visualization
    Key Features High Sensitivity: Capable of detecting low levels of RNA.
    Room Temperature Preparation: Sample preparation can be done at room temperature.
    Reduced Non-Specific Amplification: Initial incubation at 95°C activates the Taq enzyme, reducing non-specific amplification.
    Probe Detection: Optimized for use with TaqMan, Scorpions, and molecular beacon probes.
    Broad Compatibility: Compatible with standard and fast cycling protocols.
    Bulk Packaging: Available in 25ml (2500 reactions) and 50ml (5000 reactions) bottle sizes.
    Usage RNA Input: 10pg to 100ng total RNA per reaction (or a minimum of 0.01pg mRNA per reaction).
    Reverse Transcription: Recommended incubation at 50°C for 10 minutes.
    PCR Cycling Conditions: Initial denaturation at 95°C for 2 minutes, followed by 30-40 cycles of 95°C for 5 seconds (denaturation) and 60-65°C for 20-30 seconds (annealing/extension).
    Applications Gene expression analysis & single pathogen detection
    Quantification of specific target RNA from total RNA or mRNA.
    Suitable for research purposes only, not intended for therapeutic or diagnostic use.

    Accuris qMAX Probe One Step Multiplex Kit

    Feature Details
    Components 2X qPCR Master Mix
    20X Reverse Transcriptase
    RNase Inhibitors
    Accuris Hot Start Taq DNA Polymerase
    Inert Blue Dye for pipetting and visualization
    Key Features High Sensitivity: Capable of detecting low levels of RNA.
    Room Temperature Preparation: Sample preparation can be done at room temperature.
    Reduced Non-Specific Amplification: Initial incubation at 95°C activates the Taq enzyme, reducing non-specific amplification.
    Multiplex Detection: Optimized for efficient probe-based detection of multiple targets in a single reaction well.
    Broad Compatibility: Compatible with standard and fast cycling protocols.
    Bulk Packaging: Available in 25ml (2500 reactions) and 50ml (5000 reactions) bottle sizes.
    Usage RNA Input: 10pg to 100ng total RNA per reaction (or a minimum of 0.01pg mRNA per reaction).
    Reverse Transcription: Recommended incubation at 50°C for 10 minutes.
    PCR Cycling Conditions: Initial denaturation at 95°C for 2 minutes, followed by 30-40 cycles of 95°C for 5 seconds (denaturation) and 60-65°C for 20-30 seconds (annealing/extension).
    Applications Gene expression analysis & multiple pathogen detection
    Quantification of multiple RNA targets from total RNA or mRNA.
    Suitable for research purposes only, not intended for therapeutic or diagnostic use.

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