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5M Betaine Solution (PCR Grade) Buffers & Solutions Molecular Depot
5M Betaine Solution (PCR Grade) Buffers & Solutions Molecular Depot
5M Betaine Solution (PCR Grade) Buffers & Solutions Molecular Depot
5M Betaine Solution (PCR Grade) Buffers & Solutions Molecular Depot

5M Betaine Solution (PCR Grade)

$250.00

    Catalog Number: B2010141 (5 x 2 mL)

    5M Betaine Solution PCR Grade consist of a 5M stock solution of betaine (also known as trimethylglycine or N,N,N triethylammonium acetate) which is an analog of glycine with three methyl groups. Betaine is useful in PCR applications because it has an effect on the melting temperature difference between GC and AT base pairs in DNA. Custom bulk orders of this product are available upon request.

    Products are for in vitro research use only (RUO).

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    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

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What's Included

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VR Headset

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2 Touch Controllers

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Charging Cable

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Power Adapter

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Glasses Spacer

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5M Betaine Solution (PCR Grade)

Catalog number: B2010141
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 5 x 2 mL
pH: na
Supplied as: Ready-to-use solution
Appearance: Clear solution
Applications: stock solution of betaine (also known as trimethylglycine or N,N,N triethylammonium acetate) which is an analog of glycine with three methyl groups. Betaine is useful in PCR applications because it has an effect on the melting temperature difference between GC and AT base pairs in DNA.
Storage: 2-8°C
Keywords: Betaine solution, Betaine PCR
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

5M Betaine Solution (PCR Grade) is a biotechnology-grade reagent formulated to enhance the performance of polymerase chain reaction (PCR) assays, especially those involving GC-rich templates. Betaine, also known as trimethylglycine, reduces the melting temperature differential between GC and AT base pairs, improving primer annealing and amplification fidelity. Supplied as a ready-to-use clear solution in five 2 mL aliquots, this stock is ideal for optimizing PCR conditions in molecular biology, genotyping, and diagnostic workflows.

Researchers working in genomics, epigenetics, and clinical diagnostics rely on betaine to overcome secondary structure formation and improve amplification efficiency. This reagent is filtered through 0.22 µm membranes and prepared with ultrapure water to ensure high purity and minimal background interference. Stable at 2–8°C, it integrates seamlessly into manual and automated PCR protocols. Whether you're troubleshooting difficult templates or refining assay sensitivity, this solution provides a reliable tool for consistent and high-yield amplification.

Key benefits:

  • 5M betaine solution for PCR optimization and GC-rich template amplification

  • Reduces melting temperature differences between GC and AT base pairs

  • Supplied in 5 × 2 mL aliquots for flexible use

  • Filtered through 0.22 µm membranes using ultrapure water

  • Stored at 2–8°C for long-term stability and reproducibility

Products are for in vitro research use only (RUO).

References

  • 1: Day CR, Kempson SA. Betaine chemistry, roles, and potential use in liver disease. Biochim Biophys Acta. 2016 Jun;1860(6):1098-106.

     

  • 2: Figueroa-Soto CG, Valenzuela-Soto EM. Glycine betaine rather than acting only as an osmolyte also plays a role as regulator in cellular metabolism. Biochimie.
    2018 Apr;147:89-97.

     

  • 3: Zou H, Chen N, Shi M, Xian M, Song Y, Liu J. The metabolism and biotechnological application of betaine in microorganism. Appl Microbiol Biotechnol. 2016 May;100(9):3865-76.

     

  • 4: Servillo L, D’Onofrio N, Giovane A, Casale R, Cautela D, Ferrari G, Castaldo D, Balestrieri ML. The betaine profile of cereal flours unveils new and uncommon betaines. Food Chem. 2018 Jan 15;239:234-241.

     

  • 5: Koistinen VM, Kärkkäinen O, Borewicz K, Zarei I, Jokkala J, Micard V, Rosa-
    Sibakov N, Auriola S, Aura AM, Smidt H, Hanhineva K. Contribution of gut microbiota to metabolism of dietary glycine betaine in mice and in vitro colonic fermentation. Microbiome. 2019 Jul 10;7(1):103.

     

  • 6: Su Y, Guo QQ, Wang S, Zhang X, Wang J. Effects of betaine supplementation on L-threonine fed-batch fermentation by Escherichia coli. Bioprocess Biosyst Eng.
    2018 Oct;41(10):1509-1518.

     

  • 7: Luo GC, Yi TT, Jiang B, Guo XL, Zhang GY. Betaine-assisted recombinase
    polymerase assay with enhanced specificity. Anal Biochem. 2019 Jun 15;575:36-39.

     

  • 8: Cabezón FA, Stewart KR, Schinckel AP, Barnes W, Boyd RD, Wilcock P, Woodliff
    J. Effect of natural betaine on estimates of semen quality in mature AI boars during summer heat stress. Anim Reprod Sci. 2016 Jul;170:25-37.

     

  • 9: Lever M, Slow S. The clinical significance of betaine, an osmolyte with a key role in methyl group metabolism. Clin Biochem. 2010 Jun;43(9):732-44.

     

  • 10: Bhojane PP, Duff MR Jr, Bafna K, Rimmer GP, Agarwal PK, Howell EE. Aspects of Weak Interactions between Folate and Glycine Betaine. Biochemistry. 2016 Nov
    15;55(45):6282-6294.

     

  • 11: López-Rojas R, Fernández-Cuenca F, Serrano-Rocha L, Pascual Á. In vitro
    activity of a polyhexanide-betaine solution against high-risk clones of multidrug-resistant nosocomial pathogens. Enferm Infecc Microbiol Clin. 2017
    Jan;35(1):12-19.

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    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

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    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

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    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

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    By ordering, you waive all claims arising from use of Molecular Depot products.

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    MD 1000: Non-regulated use, no change notification

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Product Resources:

Manual Manual
Brochure Brochure

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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