{"product_id":"mouse-igg-magnetic-beads-1-ml","title":"Mouse IgG Magnetic Beads, 1 mL","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eMouse IgG Magnetic Beads – Catalog #B2025460\u003c\/h2\u003e\n\u003cp\u003eMouse IgG Magnetic Beads (Catalog #B2025460) are superparamagnetic nanoparticles (typically 100–500 nm) functionalized with purified mouse immunoglobulin G antibodies on their surface. Supplied as a 1 mL suspension, they provide a rapid, high-throughput platform for immunoprecipitation, antigen capture, and protein purification from cell lysates and biological samples. The magnetic core allows simple, tool-free separation with an external permanent magnet, recovering bound complexes in seconds. These beads are ideal for downstream applications such as proteomics, biomarker discovery, immune cell isolation, and antibody-based affinity capture.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025460\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eComposition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuperparamagnetic iron oxide nanoparticles coated with mouse IgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eTypically 100–500 nm (per manufacturer)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface coating:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePurified mouse immunoglobulin G (IgG)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAqueous suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eImmunoprecipitation, antigen capture, protein purification, biomarker discovery, immune cell isolation, magnetic separation-based diagnostics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMouse IgG magnetic beads, magnetic nanoparticles, IgG-coated beads, immunomagnetic particles, superparamagnetic beads, magnetic immunoseparation, antibody-coated magnetic nanoparticles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eMagnetic separation using functionalized nanoparticles is a powerful technique in biotechnology and research. Superparamagnetic iron oxide nanoparticles (SPIONs) respond instantly to external magnetic fields but retain no residual magnetization in the absence of the field, making them ideal for rapid, reversible capture and release of biomolecules. When coated with mouse IgG antibodies, these beads can bind and capture antigens or antibody-binding molecules from complex samples. The mouse IgG coating provides a versatile affinity surface for direct antibody-antigen interactions or, alternatively, for indirect capture via secondary antibodies or protein A\/G. The rapid magnetic separation (typically \u0026lt;30 seconds with an external magnet) improves workflow efficiency and reduces sample loss compared to traditional centrifugation or filtration methods.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eImmunoprecipitation (IP) of specific antigens from cell lysates or biological fluids\u003c\/li\u003e\n\u003cli\u003eIsolation of immune cell subsets using antibodies against surface markers\u003c\/li\u003e\n\u003cli\u003eRapid purification of recombinant proteins tagged with epitopes recognized by mouse antibodies\u003c\/li\u003e\n\u003cli\u003eMagnetic bead-based diagnostics and biomarker enrichment\u003c\/li\u003e\n\u003cli\u003eCo-immunoprecipitation (co-IP) for protein-protein interaction studies\u003c\/li\u003e\n\u003cli\u003eSample preparation for downstream mass spectrometry (proteomics) analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eGently mix the suspension before use to resuspend the magnetic beads. Add the appropriate volume of beads to your sample (typically 10–100 μL per 100 μL sample, per the manufacturer's recommendation) or incubate the beads with your antibody solution if performing indirect capture. After incubation with your sample (typically 10 minutes to 2 hours, depending on your protocol), place the tube in a magnetic stand or magnetic separator for 1–2 minutes; the beads will collect at the tube wall, and the supernatant can be discarded. Wash the beads with appropriate wash buffer (e.g., PBS, TBST) by resuspending and re-separating. Repeat wash steps as needed for your application. Finally, elute the bound material with an elution buffer (e.g., 100 mM glycine pH 2.8 or appropriate detergent-containing buffer) or boiling in loading buffer for direct SDS-PAGE analysis.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncubation conditions:\u003c\/strong\u003e Room temperature (4–25°C) is typical; room temperature incubation may increase binding kinetics. Some protocols benefit from 4°C overnight incubation for maximum binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Use a permanent magnet stand or magnetic separator designed for microtubes; separation is typically complete in 1–2 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Perform 2–4 wash steps with 200–500 μL wash buffer (PBS or TBST) for each wash, resuspending beads between steps and re-separating.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Choose an elution buffer compatible with your downstream application (e.g., pH 2.8 glycine for HPLC\/mass spectrometry, SDS sample buffer for Western blot, or nucleic acid lysis buffer for RNA\/DNA work).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage of beads:\u003c\/strong\u003e Keep at 2–8°C; avoid freezing, which may damage the bead suspension.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 mL of Mouse IgG-coated superparamagnetic nanoparticles\u003c\/li\u003e\n\u003cli\u003eReady-to-use suspension with no additional preparation required\u003c\/li\u003e\n\u003cli\u003eHigh surface area and rapid magnetic responsiveness\u003c\/li\u003e\n\u003cli\u003eSufficient volume for multiple immunoprecipitation or capture experiments\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eRapid magnetic separation (seconds) vs. centrifugation (minutes)\u003c\/li\u003e\n\u003cli\u003eReduces sample loss and improves recovery of bound complexes\u003c\/li\u003e\n\u003cli\u003eMouse IgG coating provides universal antibody-binding capability\u003c\/li\u003e\n\u003cli\u003eSuperparamagnetic properties ensure complete release of beads when field is removed\u003c\/li\u003e\n\u003cli\u003eCompatible with high-throughput workflow and robotic sample preparation\u003c\/li\u003e\n\u003cli\u003eReady-to-use format; no additional bead preparation needed\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between magnetic beads with mouse IgG versus other coatings?\u003c\/strong\u003e\u003cbr\u003eMouse IgG provides a universal capture surface for any antigen that binds mouse antibodies. Other coatings (e.g., Protein A, Protein G, streptavidin) bind specific antibody types or biotinylated molecules. Mouse IgG beads are versatile for indirect capture via secondary antibodies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads with non-mouse antibodies?\u003c\/strong\u003e\u003cbr\u003eYes, indirectly. If your primary antibody is from another species (e.g., rabbit), add a secondary antibody (mouse anti-rabbit) to bridge the interaction, or use Protein A\/G beads instead.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long should I incubate the beads with my sample?\u003c\/strong\u003e\u003cbr\u003eTypically 10 minutes to 2 hours at room temperature, or overnight at 4°C. Optimize the incubation time and bead volume for your specific antigen and antibody.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I freeze the beads?\u003c\/strong\u003e\u003cbr\u003eNo; freezing may damage the bead suspension and reduce functionality. Keep at 2–8°C and use within the shelf-life specified on the vial.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the best magnetic separator to use?\u003c\/strong\u003e\u003cbr\u003eAny permanent magnet stand or magnetic separator designed for 1.5–2 mL microtubes will work. Most commercial tube-based and 96-well plate magnetic separators are compatible.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eVedarethinam V, Jeevanandam J, Acquah C, Danquah MK. Magnetic Nanoparticles for Protein Separation and Purification. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2023;2699:125-159.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-3362-5_8\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLe TD, Suttikhana I, Ashaolu TJ. State of the art on the separation and purification of proteins by magnetic nanoparticles. \u003cem\u003eJ Nanobiotechnology.\u003c\/em\u003e 2023;21(1):363.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1186\/s12951-023-02123-7\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGu H, Xu K, Xu C, Xu B. Biofunctional magnetic nanoparticles for protein separation and pathogen detection. \u003cem\u003eChem Commun (Camb).\u003c\/em\u003e 2006;(9):941-9.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1039\/b514130c\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813632209194,"sku":"BTS-B2025460","price":1185.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025460.png?v=1790900000","url":"https:\/\/bluetigerscientific.com\/products\/mouse-igg-magnetic-beads-1-ml","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}