{"product_id":"mdykdhdgdykdhdidykddddk-peptide-10-mg-1","title":"MDYKDHDGDYKDHDIDYKDDDDK Peptide, 10 mg","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eMDYKDHDGDYKDHDIDYKDDDDK Peptide – Catalog #B2026675\u003c\/h2\u003e\n\u003cp\u003eMDYKDHDGDYKDHDIDYKDDDDK (Catalog #B2026675) is a 24-amino-acid synthetic peptide with a molecular weight of 2861.87 Da. The sequence contains repeating aspartate-tyrosine-lysine (DYK) epitope motifs interspersed with additional charged residues (glutamate), and terminates with the FLAG octapeptide epitope (DYKDDDDK). This composition makes it valuable as a control peptide, immunogen, or detection standard in applications requiring epitope recognition, antibody development, or peptide-based protein tagging. Supplied as 10 mg of lyophilized powder.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2026675\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e10 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSequence:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMDYKDHDGDYKDHDIDYKDDDDK\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2861.87 g\/mol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eNumber of amino acids:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e24\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eImmunogen for antibody development, positive control in immunoassays, protein tagging standard, epitope mapping, peptide binding assays, synthetic peptide standard\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e–20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSynthetic peptide, FLAG epitope, DYK motif, DYKDDDDK, FLAG tag, peptide standard, immunogen, control peptide, epitope tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eThe FLAG octapeptide epitope (DYKDDDDK) is one of the most widely used peptide tags in molecular biology. It is recognized by multiple anti-FLAG monoclonal antibodies with high specificity and is used to label and purify recombinant proteins, detect transgenes, and perform immunoaffinity chromatography. The MDYKDHDGDYKDHDIDYKDDDDK sequence contains multiple DYK motifs in tandem, which may serve as repeating epitope units or immunogenic structures in immunological assays. The presence of both charged (glutamate, aspartate) and aromatic (tyrosine) residues provides hydrophilicity and potential for multivalent antibody recognition. As a synthetic peptide, it serves as an excellent positive control, immunogen substrate, or binding standard for assay development and validation.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003ePositive control or calibration standard in anti-FLAG antibody assays and ELISA\u003c\/li\u003e\n\u003cli\u003eImmunogen for raising anti-FLAG or anti-epitope antibodies in rabbits, mice, or other hosts\u003c\/li\u003e\n\u003cli\u003eStandard in epitope mapping and peptide-antibody interaction studies\u003c\/li\u003e\n\u003cli\u003eCoating antigen for protein-peptide binding assays\u003c\/li\u003e\n\u003cli\u003eQuality control reference for peptide synthesis and mass spectrometry validation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eReconstitute the lyophilized powder in ultrapure water, phosphate-buffered saline (PBS), or your chosen assay buffer. The peptide is freely soluble in aqueous solutions and most common biochemical buffers. Start with a concentration suitable for your assay (for example, 1–100 μM for immunoassays). Store reconstituted solutions at 2–8°C if used within days, or at –20°C for longer storage. Avoid repeated freeze-thaw cycles by preparing aliquots.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e Readily soluble in water and aqueous buffers at physiological pH.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e The lyophilized powder is stable for years at –20°C; once reconstituted, use within weeks (store at 2–8°C) or months (at –20°C).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration recommendations:\u003c\/strong\u003e For immunoassays, 1–10 μM; for immunization, 1–10 mg\/mL in aqueous or saline buffer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity check:\u003c\/strong\u003e You can verify the peptide by mass spectrometry (expected MH+ = 2862.87 m\/z) or high-performance liquid chromatography (HPLC).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e10 mg of lyophilized MDYKDHDGDYKDHDIDYKDDDDK peptide\u003c\/li\u003e\n\u003cli\u003eHigh-purity synthetic peptide (biotechnology grade)\u003c\/li\u003e\n\u003cli\u003eMolecular weight: 2861.87 Da\u003c\/li\u003e\n\u003cli\u003eReady to reconstitute and use in immunological or binding assays\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eContains the widely-recognized FLAG epitope recognized by anti-FLAG monoclonal antibodies\u003c\/li\u003e\n\u003cli\u003eRepeating DYK motif design for potential multivalent antibody engagement\u003c\/li\u003e\n\u003cli\u003eHigh purity and well-defined chemical composition\u003c\/li\u003e\n\u003cli\u003eSuitable as immunogen, control, or assay standard\u003c\/li\u003e\n\u003cli\u003eLyophilized format for convenient storage and long shelf-life\u003c\/li\u003e\n\u003cli\u003eExcellent positive control for validating anti-FLAG detection methods\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the FLAG epitope?\u003c\/strong\u003e\u003cbr\u003eFLAG is the peptide sequence DYKDDDDK, recognized with high affinity and specificity by anti-FLAG monoclonal antibodies (e.g., M1, M2). It is widely used to tag recombinant proteins for detection and purification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWill anti-FLAG antibodies recognize this peptide?\u003c\/strong\u003e\u003cbr\u003eYes. This peptide contains the complete DYKDDDDK epitope and can be used as a positive control or standard with anti-FLAG antibodies in ELISA, Western blot, immunoprecipitation, and immunofluorescence.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use this as an immunogen to generate antibodies?\u003c\/strong\u003e\u003cbr\u003eYes. At 1–10 mg\/mL in saline or PBS, the peptide is suitable for immunization of rabbits, mice, or other hosts to raise antibodies against the FLAG tag or DYK epitope.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I store the lyophilized peptide?\u003c\/strong\u003e\u003cbr\u003eKeep the sealed vial at –20°C. Once reconstituted, store solutions at 2–8°C (for short-term use, up to a few weeks) or –20°C (for longer storage); avoid repeated freeze-thaw cycles.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the expected mass?\u003c\/strong\u003e\u003cbr\u003eThe peptide has a calculated molecular weight of 2861.87 Da. You can verify this using mass spectrometry, which should show an [M+H]+ ion at approximately 2862.87 m\/z.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKnappik A, Plückthun A. An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments. \u003cem\u003eBiotechniques.\u003c\/em\u003e 1994;17(4):754-61.\u003c\/span\u003e\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/7530459\/\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSlootstra JW, Kuperus D, Plückthun A, Meloen RH. Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5. \u003cem\u003eMol Divers.\u003c\/em\u003e 1997;2(3):156-64.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/BF01682203\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKrishnan G, Morabito MA, Moczydlowski E. Expression and characterization of Flag-epitope- and hexahistidine-tagged derivatives of saxiphilin for use in detection and assay of saxitoxin. \u003cem\u003eToxicon.\u003c\/em\u003e 2001;39(2-3):291-301.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0041-0101(00)00127-6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813633323306,"sku":"BTS-B2026675","price":935.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2026675.png?v=1790900040","url":"https:\/\/bluetigerscientific.com\/products\/mdykdhdgdykdhdidykddddk-peptide-10-mg-1","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}