{"product_id":"low-density-glyoxal-6bcl-25-ml","title":"Low Density Glyoxal 6BCL, 25 mL","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eLow Density Glyoxal 6BCL, 6% Highly-Crosslinked Agarose – Catalog #B2025396\u003c\/h2\u003e\n\u003cp\u003eLow Density Glyoxal 6BCL (Catalog #B2025396) is a pre-activated affinity chromatography resin consisting of 6% highly-crosslinked (BCL) agarose beads bearing low-density glyoxal (aldehyde) functional groups. The \"6BCL\" designation indicates a 6% agarose matrix with enhanced cross-linking for improved mechanical rigidity and chemical stability. The glyoxal groups couple directly to lysine and N-terminal amino groups on proteins, peptides, and enzymes, forming stable Schiff base covalent bonds without requiring additional activation reagents. Supplied as 25 mL of resin, it provides a ready-to-use platform for enzyme immobilization, affinity purification, and immunoaffinity applications.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025396\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAgarose matrix:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e6% highly-crosslinked agarose (6BCL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eFunctional group density:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLow (per manufacturer)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eActivation:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePre-activated with glyoxal aldehyde groups\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eEnzyme immobilization, affinity purification, enzyme stabilization, immunoaffinity chromatography, protein coupling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLow density glyoxal 6BCL, glyoxal agarose 6BCL, aldehyde-activated agarose, 6% crosslinked agarose, enzyme immobilization resin, affinity chromatography support, Schiff base coupling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eGlyoxal-activated agarose resins are widely used in affinity chromatography and enzyme immobilization because they provide simple, efficient coupling chemistry under mild conditions. The glyoxal aldehyde group reacts with primary amino groups (lysine and N-terminal amine) to form Schiff bases, which are initially reversible but can be stabilized by reduction with borohydride reagents to form secondary amine (imine-reduced) linkages. The 6BCL matrix offers higher mechanical rigidity and chemical stability than lower-crosslink resins, making it suitable for high-flow applications and extended chromatographic runs. Low-density functional groups reduce steric hindrance and can improve binding kinetics and selectivity for some applications.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eEnzyme immobilization for biocatalysis and continuous-flow bioreactors\u003c\/li\u003e\n\u003cli\u003ePreparation of affinity columns for purification of target proteins or antigens\u003c\/li\u003e\n\u003cli\u003eCreation of immunoaffinity columns with antibodies or antibody fragments\u003c\/li\u003e\n\u003cli\u003eEnzyme stabilization and storage in immobilized form\u003c\/li\u003e\n\u003cli\u003eCapture and concentration of low-abundance proteins from complex samples\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the resin suspension at 2–8°C. Before use, gently mix to resuspend and equilibrate a chromatography column with coupling buffer (typically pH 7–8, such as 10 mM sodium phosphate buffer). Dissolve or dilute your target ligand (protein, enzyme, antibody) in the same buffer and apply it to the column; the glyoxal groups will react with the ligand's amino groups. Incubate the column for the recommended time (typically 15 minutes to several hours, depending on the ligand and desired coupling efficiency), then wash thoroughly with coupling buffer followed by stringent wash buffer if needed.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eLigand loading:\u003c\/strong\u003e Typical range is 1–10 mg protein per mL of resin; optimize for your specific application and ligand.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003epH optimum:\u003c\/strong\u003e Coupling is most efficient at pH 7–8; the reaction rate increases with pH up to about 8, then plateaus.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReducing conditions (optional):\u003c\/strong\u003e To convert Schiff bases to stable secondary amines, treat the coupled resin with NaBH₄ or NaBH(OAc)₃ and incubate for 30 minutes to 2 hours at 4°C or room temperature.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage of coupled resin:\u003c\/strong\u003e Keep at 2–8°C; add 0.01–0.1% sodium azide as a preservative for long-term storage.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegeneration:\u003c\/strong\u003e The column can often be regenerated and re-used by washing with appropriate stringent buffers, depending on your application.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 mL of pre-activated Low Density Glyoxal 6BCL agarose resin\u003c\/li\u003e\n\u003cli\u003e6% highly-crosslinked agarose (6BCL) for improved mechanical stability\u003c\/li\u003e\n\u003cli\u003ePre-activated with glyoxal aldehyde groups; ready to use\u003c\/li\u003e\n\u003cli\u003eSufficient material for multiple immobilization experiments or columns\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eSimple coupling chemistry with no additional activation reagents required\u003c\/li\u003e\n\u003cli\u003eMild conditions preserve protein structure and activity\u003c\/li\u003e\n\u003cli\u003eHighly-crosslinked agarose (6BCL) offers superior mechanical strength and chemical stability\u003c\/li\u003e\n\u003cli\u003eLow-density aldehyde groups minimize steric hindrance\u003c\/li\u003e\n\u003cli\u003eSchiff bases can be reduced for permanent, stable linkages\u003c\/li\u003e\n\u003cli\u003eSuitable for high-flow and long-term chromatographic applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the 6BCL designation?\u003c\/strong\u003e\u003cbr\u003e6BCL means 6% agarose with \"best cross-linking\" (BCL), indicating enhanced cross-linking that provides higher mechanical rigidity, better flow characteristics, and greater chemical stability compared to less-crosslinked matrices.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much ligand should I immobilize?\u003c\/strong\u003e\u003cbr\u003eA starting point is 1–10 mg protein per mL of resin. Higher loading increases the column's capacity but may reduce selectivity or cause crowding of ligands; optimize empirically for your target.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDo I need to reduce the Schiff base?\u003c\/strong\u003e\u003cbr\u003eSchiff bases are stable for most applications; reduction with borohydride is optional. Reduce if you want a more stable linkage for long-term storage or extended chromatographic use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I regenerate and reuse this column?\u003c\/strong\u003e\u003cbr\u003eYes, many columns can be regenerated and reused if the ligand is robust; use appropriate stringent wash buffers (e.g., high salt, detergent, or pH extremes, depending on your application).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between 6 and 6BCL?\u003c\/strong\u003e\u003cbr\u003e6BCL has additional cross-linking, providing better mechanical properties and flow characteristics. Use 6BCL for applications requiring high flow rates or extended runs.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLuong JH, Scouten WH. Affinity purification of natural ligands. \u003cem\u003eCurr Protoc Protein Sci.\u003c\/em\u003e 2008;Chapter 9:Unit 9.3.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/0471140864.ps0903s52\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eWang Y, Zhang X, Han N, Wu Y, Wei D. Oriented covalent immobilization of recombinant protein A on the glutaraldehyde activated agarose support. \u003cem\u003eInt J Biol Macromol.\u003c\/em\u003e 2018;120(Pt A):100-108.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.ijbiomac.2018.08.074\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003ePepys MB, Dash AC, Ashley MJ. Isolation of C-reactive protein by affinity chromatography. \u003cem\u003eClin Exp Immunol.\u003c\/em\u003e 1977;30(1):32-7.\u003c\/span\u003e\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23915\/\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813630406954,"sku":"BTS-B2025396","price":1185.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025396.png?v=1790899962","url":"https:\/\/bluetigerscientific.com\/products\/low-density-glyoxal-6bcl-25-ml","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}