{"product_id":"low-density-glyoxal-6-25-ml","title":"Low Density Glyoxal 6, 25 mL","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eLow Density Glyoxal 6, 6% Agarose – Catalog #B2025416\u003c\/h2\u003e\n\u003cp\u003eLow Density Glyoxal 6 (Catalog #B2025416) is a pre-activated agarose affinity chromatography resin composed of 6% crosslinked agarose beads bearing low-density glyoxal (aldehyde) functional groups. These activated glyoxal groups react directly with primary amino groups (lysine and N-terminal amine) on proteins, peptides, and other ligands to form stable Schiff base covalent bonds, without requiring additional coupling chemistry or intermediate activation steps. Supplied as 25 mL of resin, it is ideal for affinity purification, enzyme immobilization, and the preparation of immunoaffinity columns.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025416\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAgarose matrix:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e6% crosslinked agarose (6BCL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eFunctional group density:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLow (per manufacturer)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eActivation:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePre-activated with glyoxal aldehyde groups\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity purification, enzyme immobilization, enzyme stabilization, immunoaffinity chromatography, protein purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLow density glyoxal 6, glyoxal agarose, aldehyde-activated agarose, affinity chromatography resin, enzyme immobilization resin, protein purification resin, Schiff base coupling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eGlyoxal (1,2-ethanedial) is a two-carbon aldehyde that reacts with primary amino groups to form stable Schiff bases (imines) and, under certain conditions, secondary amine products. In affinity chromatography, glyoxal-activated agarose resins are useful alternatives to other pre-activated matrices because the coupling reaction is simple, proceeds efficiently at mild pH and temperature, and the resulting linkages are covalent and stable through typical chromatographic procedures. The \"low density\" designation indicates a lower concentration of glyoxal groups, which can be advantageous for applications requiring reduced steric hindrance or lower ligand loading. The 6% agarose backbone provides mechanical stability and flow characteristics suitable for column packing and gentle protein handling.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eImmobilization of enzymes for biocatalytic reactors and affinity-based enzyme assays\u003c\/li\u003e\n\u003cli\u003ePreparation of antibody columns for immunoaffinity purification of antigens\u003c\/li\u003e\n\u003cli\u003ePurification of glycoproteins and other ligand-binding molecules\u003c\/li\u003e\n\u003cli\u003eEnzyme stabilization and storage\u003c\/li\u003e\n\u003cli\u003eTeaching laboratory demonstrations of affinity chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the resin suspension at 2–8°C. Before use, gently resuspend and equilibrate the column with coupling buffer (typically 10 mM sodium phosphate pH 7.0 to 8.0, or similar). Dissolve or dilute your target ligand in the same buffer and apply it to the column; the glyoxal groups will couple to the ligand's lysine and N-terminal amino groups. After coupling, wash the column with the coupling buffer, then with stringent wash buffer if needed.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eLigand concentration:\u003c\/strong\u003e Follow the manufacturer's recommendations for optimal loading; typically 1–10 mg protein per mL of resin.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003epH:\u003c\/strong\u003e Coupling is typically carried out at pH 7–8; lower pH favors Schiff base formation, while higher pH may promote secondary amine products.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncubation:\u003c\/strong\u003e Allow sufficient contact time (minutes to hours, depending on your protocol) for the coupling reaction to reach completion.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReducing conditions:\u003c\/strong\u003e If a stable secondary amine linkage is desired, reduce the Schiff base with NaBH₄ or NaBH(OAc)₃ after coupling.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage after coupling:\u003c\/strong\u003e Keep the ligand-bound resin at 2–8°C; add 0.01–0.1% sodium azide as a preservative if storing for extended periods.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 mL of pre-activated Low Density Glyoxal 6 agarose resin\u003c\/li\u003e\n\u003cli\u003ePre-activated with glyoxal aldehyde groups; no separate activation chemistry required\u003c\/li\u003e\n\u003cli\u003eEnough material for multiple columns or immobilization experiments\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eSimple, efficient coupling reaction with mild conditions\u003c\/li\u003e\n\u003cli\u003ePre-activated; no need for separate coupling reagents or chemical activation steps\u003c\/li\u003e\n\u003cli\u003eStable covalent linkages suitable for repeated chromatographic use\u003c\/li\u003e\n\u003cli\u003eLow ligand density reduces steric hindrance and improves binding kinetics for some applications\u003c\/li\u003e\n\u003cli\u003eBiocompatible agarose matrix for gentle protein handling\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between glyoxal and glutaraldehyde activated resins?\u003c\/strong\u003e\u003cbr\u003eBoth form Schiff bases with lysine; glyoxal is a two-carbon aldehyde and typically requires reducing conditions for stable secondary amine products, while glutaraldehyde (five carbons) can form more complex cross-links and polymers. Glyoxal offers simpler, more predictable chemistry.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I know how much ligand to couple?\u003c\/strong\u003e\u003cbr\u003eA typical starting point is 1–10 mg protein per mL of resin. Optimize loading for your specific application; higher loading increases capacity but may reduce selectivity or cause steric clashes in the column.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reduce the Schiff base for extra stability?\u003c\/strong\u003e\u003cbr\u003eYes. After coupling, reduce the imine with NaBH₄ (aqueous) or NaBH(OAc)₃ (aqueous or organic) to form stable secondary amine linkages; follow standard borohydride safety protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIs this resin suitable for purifying my specific protein?\u003c\/strong\u003e\u003cbr\u003eGlyoxal coupling is general and works with any protein or peptide containing lysine or free N-terminal amine. Request a COA or contact us to discuss your specific application.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I store the coupled resin after preparing my column?\u003c\/strong\u003e\u003cbr\u003eKeep at 2–8°C and add 0.01–0.1% sodium azide to prevent microbial growth during storage.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLuong JH, Scouten WH. Affinity purification of natural ligands. \u003cem\u003eCurr Protoc Protein Sci.\u003c\/em\u003e 2008;Chapter 9:Unit 9.3.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/0471140864.ps0903s52\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eWang Y, Zhang X, Han N, Wu Y, Wei D. Oriented covalent immobilization of recombinant protein A on the glutaraldehyde activated agarose support. \u003cem\u003eInt J Biol Macromol.\u003c\/em\u003e 2018;120(Pt A):100-108.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.ijbiomac.2018.08.074\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003ePepys MB, Dash AC, Ashley MJ. Isolation of C-reactive protein by affinity chromatography. \u003cem\u003eClin Exp Immunol.\u003c\/em\u003e 1977;30(1):32-7.\u003c\/span\u003e\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23915\/\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813631422762,"sku":"BTS-B2025416","price":1205.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025416.png?v=1790899983","url":"https:\/\/bluetigerscientific.com\/products\/low-density-glyoxal-6-25-ml","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}