{"product_id":"low-density-glyoxal-4bcl-agarose-4-cross-linked","title":"Low Density Glyoxal 4BCL Agarose (4% Cross-linked)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eLow Density Glyoxal 4BCL Agarose (4% Cross-linked) – Catalog #B2025406\u003c\/h2\u003e\n\u003cp\u003eLow Density Glyoxal 4BCL Agarose (Catalog #B2025406) is a pre-activated affinity chromatography resin on 4% cross-linked agarose beads bearing aldehyde (glyoxal) functional groups at a deliberately low density. The 4BCL designation refers to the cross-linking chemistry of the agarose backbone. Like other glyoxal-activated resins, it enables stable covalent immobilization of proteins and enzymes via direct reaction with lysine amino groups, forming reversible Schiff bases that can be reduced to permanent secondary amine bonds. The low density of reactive groups minimizes steric effects and protein aggregation.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025406\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAgarose composition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e4% cross-linked agarose (4BCL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eFunctional group:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlyoxal (aldehyde, −CHO); low density\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCoupling mechanism:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCovalent via Schiff base formation with lysine amino groups (reducible)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eEnzyme immobilization and reactor columns, antibody coupling, immunoaffinity purification, enzyme stabilization via multipoint attachment, diagnostic and research assay matrices\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlyoxal 4BCL, low density glyoxal, glyoxyl-activated agarose, pre-activated resin, covalent immobilization, enzyme coupling, affinity chromatography\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eGlyoxal-activated agarose resins are among the most widely used supports for covalent protein immobilization in research and biotechnology. The glyoxal (aldehyde) groups react specifically with primary amino groups, particularly lysine residues at physiological and slightly alkaline pH values. The resulting Schiff base is initially reversible but becomes a stable secondary amine bond when reduced with sodium borohydride (NaBH₄) or sodium cyanoborohydride (NaCNBH₃). The low density of glyoxal groups on this resin minimizes steric hindrance and the risk of excessive cross-linking, allowing more controlled, uniform coupling of proteins and maintenance of biological activity.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eCovalent immobilization of enzymes for affinity chromatography and biocatalysis\u003c\/li\u003e\n\u003cli\u003eAntibody or ligand coupling for immunoaffinity purification and diagnostic assays\u003c\/li\u003e\n\u003cli\u003eEnzyme reactor columns for continuous bioprocessing\u003c\/li\u003e\n\u003cli\u003eProtein stabilization through multipoint attachment\u003c\/li\u003e\n\u003cli\u003eDevelopment and validation of affinity separation methods\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the resin suspension at 2–8°C. Before use, inspect for excessive yellowing, which indicates hydrolysis of glyoxal groups. Gently resuspend the resin and pour into a column or incubate as a slurry for batch coupling. For column mode, pack according to standard procedures. To couple protein or enzyme, incubate the resin with your target molecule in an appropriate buffer (pH 7–9; e.g., 0.1 M phosphate pH 7.0–8.0 or 0.1 M carbonate pH 9.0) for 2–4 hours at room temperature or overnight at 4°C. Optimize protein concentration empirically (typically 1–10 mg\/mL). After coupling, reduce the Schiff base with sodium borohydride or sodium cyanoborohydride following standard safety protocols, then wash thoroughly to remove unreacted protein and reagents.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling buffer pH:\u003c\/strong\u003e Optimal range is pH 7–9; higher pH accelerates Schiff base formation but may denature some proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein concentration:\u003c\/strong\u003e Start with 1–10 mg\/mL and optimize for your target.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling time:\u003c\/strong\u003e 2–4 hours at 25°C or overnight at 4°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReduction:\u003c\/strong\u003e Use NaBH₄ (in ethanol or dilute buffer) or NaCNBH₃ (in pH 7 buffer), following reagent-specific protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Extensive washing with buffer and water removes unreacted protein and coupling byproducts.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 mL of low-density glyoxal agarose (4BCL, 4% cross-linked)\u003c\/li\u003e\n\u003cli\u003ePre-activated resin ready for direct enzyme, antibody, or protein coupling\u003c\/li\u003e\n\u003cli\u003eEnough material for multiple immobilization experiments\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eGentle, covalent coupling chemistry that preserves protein activity\u003c\/li\u003e\n\u003cli\u003eLow glyoxal density minimizes aggregation and excessive cross-linking\u003c\/li\u003e\n\u003cli\u003eStable secondary amine bonds after reduction\u003c\/li\u003e\n\u003cli\u003eVersatile for enzymes, antibodies, and other biomolecules\u003c\/li\u003e\n\u003cli\u003eWell-established protocols and decades of literature support\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat does the \"4BCL\" designation mean?\u003c\/strong\u003e\u003cbr\u003eThe \"4\" refers to the agarose concentration (4%), and \"BCL\" indicates the cross-linking chemistry of the agarose backbone.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow is Glyoxal 4BCL different from other glyoxal resins?\u003c\/strong\u003e\u003cbr\u003eThe main differences are in the agarose cross-linking pattern (4BCL vs. others) and the density of glyoxal groups. All glyoxal resins use the same aldehyde-lysine chemistry; differences reflect particle size and activation level.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMust I reduce the Schiff base?\u003c\/strong\u003e\u003cbr\u003eFor maximum stability, reduce with NaBH₄ or NaCNBH₃. The Schiff base is reversible; reduction converts it to a permanent secondary amine bond.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat protein concentration should I use?\u003c\/strong\u003e\u003cbr\u003eTypical range is 1–10 mg\/mL. Optimize empirically based on your protein and desired coupling density.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse this resin after stripping immobilized protein?\u003c\/strong\u003e\u003cbr\u003eNot typically. Once immobilized and reduced, the protein is covalently bound. Regeneration methods (e.g., high-pH or strong chaotropes) may partially work but often damage the resin.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a COA or technical information?\u003c\/strong\u003e\u003cbr\u003eRequest a quote or contact us and we will provide available lot documentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLópez-Gallego F, Montes T, Fuentes M, Alonso N, Grazu V, Betancor L, Guisán JM, Fernández-Lafuente R. Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports. \u003cem\u003eJ Biotechnol.\u003c\/em\u003e 2005;116(1):1-10.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jbiotec.2004.09.015\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKnödler M, Rühl C, Opdensteinen P, Buyel JF. Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study. \u003cem\u003eJ Vis Exp.\u003c\/em\u003e 2019;(150).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3791\/59933\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813631160618,"sku":"BTS-B2025406","price":1195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025406.png?v=1790899977","url":"https:\/\/bluetigerscientific.com\/products\/low-density-glyoxal-4bcl-agarose-4-cross-linked","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}