{"product_id":"low-density-glyoxal-4-fine-agarose-4-cross-linked","title":"Low Density Glyoxal 4 Fine Agarose (4% Cross-linked)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eLow Density Glyoxal 4 Fine Agarose (4% Cross-linked) – Catalog #B2025400\u003c\/h2\u003e\n\u003cp\u003eLow Density Glyoxal 4 Fine Agarose (Catalog #B2025400) is a pre-activated affinity chromatography resin consisting of smaller, finer 4% cross-linked agarose particles bearing a low density of glyoxal (aldehyde) groups. The smaller particle size improves chromatographic resolution and mass transfer rates, making it especially valuable for applications demanding high purity or fine separation. Like its standard-size counterpart, it enables stable covalent immobilization of enzymes, antibodies, and ligands via Schiff base formation with lysine residues, which can be reduced for maximum stability.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025400\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAgarose composition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e4% cross-linked agarose, fine beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eFunctional group:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlyoxal (aldehyde, −CHO); low density\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eFiner\/smaller particles than standard Glyoxal 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCoupling mechanism:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCovalent via Schiff base formation with lysine (reducible or stable)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHigh-resolution enzyme immobilization, antibody coupling with improved selectivity, fine protein fractionation, immunoaffinity purification, diagnostic assay matrices\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlyoxal 4 fine, low density glyoxal fine beads, fine glyoxyl agarose, small-particle affinity resin, high-resolution chromatography, fine-bead agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eIn chromatography, particle size directly affects resolution and mass transfer kinetics. Smaller particles have a greater surface area relative to volume and diffuse distances shorter, improving both the speed of equilibration and the separation of similar molecules. The fine-bead version of glyoxal agarose retains the chemistry of standard-size beads—direct coupling to lysine residues via Schiff base formation—while offering the performance advantages of smaller particles. The low density of glyoxal groups minimizes steric hindrance and protein aggregation, allowing cleaner coupling and better recovery of biologically active immobilized proteins.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh-resolution enzyme immobilization for affinity columns\u003c\/li\u003e\n\u003cli\u003eAntibody coupling with improved selectivity and minimal aggregation\u003c\/li\u003e\n\u003cli\u003eFine fractionation of similar proteins or isoforms\u003c\/li\u003e\n\u003cli\u003eImmunoaffinity purification with improved purity\u003c\/li\u003e\n\u003cli\u003eDiagnostic and research assay development where resolution is critical\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the resin at 2–8°C. Before use, inspect for excessive yellowing, which may indicate glyoxal hydrolysis. Due to the smaller particle size, expect higher backpressure when packing columns; adjust flow rates accordingly. Pack the column gently to avoid compression. Couple proteins and ligands as with standard-size glyoxal agarose: incubate with target protein in coupling buffer (pH 7–9) for 2–4 hours or overnight at 4–25°C, then reduce the Schiff base with sodium borohydride or sodium cyanoborohydride for stability.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003ePacking:\u003c\/strong\u003e Pack gently to avoid particle compression; smaller beads pack more densely and may generate higher backpressure.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow rate:\u003c\/strong\u003e Start with lower flow rates than would be used for standard-size beads to maintain efficiency.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling:\u003c\/strong\u003e Use the same pH (7–9) and protein concentrations as with standard beads; fine beads improve resolution but do not alter coupling chemistry.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReduction:\u003c\/strong\u003e Reduce Schiff bases with NaBH₄ or NaCNBH₃ following standard protocols.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 mL of low-density glyoxal agarose with fine particle size (4% cross-linked)\u003c\/li\u003e\n\u003cli\u003ePre-activated resin ready for protein or ligand coupling\u003c\/li\u003e\n\u003cli\u003eImproved chromatographic resolution compared to standard-size beads\u003c\/li\u003e\n\u003cli\u003eEnough material for multiple high-resolution purification experiments\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eSmaller particle size improves chromatographic resolution and mass transfer\u003c\/li\u003e\n\u003cli\u003eLow glyoxal density reduces protein aggregation during coupling\u003c\/li\u003e\n\u003cli\u003eMild, covalent chemistry preserves protein activity\u003c\/li\u003e\n\u003cli\u003eApplicable to fine fractionation and high-purity applications\u003c\/li\u003e\n\u003cli\u003eSame gentle coupling chemistry as standard glyoxal agarose\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow are fine beads different from standard Glyoxal 4?\u003c\/strong\u003e\u003cbr\u003eFine beads are smaller particles that offer higher resolution and faster mass transfer. They pack more densely and may require lower flow rates, but the coupling chemistry is identical.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWill fine beads give higher resolution than standard beads?\u003c\/strong\u003e\u003cbr\u003eYes, smaller particles improve resolution. For high-precision separations (e.g., antibody isoforms or enzyme variants), fine beads are advantageous.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat flow rate should I use with fine beads?\u003c\/strong\u003e\u003cbr\u003eStart with a lower flow rate than you would use with standard beads; optimize empirically based on your column and application.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDo I still reduce the Schiff base with fine beads?\u003c\/strong\u003e\u003cbr\u003eYes. For maximum stability, reduce with NaBH₄ or NaCNBH₃ using the same protocols as for standard beads.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a COA or technical information?\u003c\/strong\u003e\u003cbr\u003eRequest a quote or contact us and we will provide available lot documentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLópez-Gallego F, Montes T, Fuentes M, Alonso N, Grazu V, Betancor L, Guisán JM, Fernández-Lafuente R. Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports. \u003cem\u003eJ Biotechnol.\u003c\/em\u003e 2005;116(1):1-10.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jbiotec.2004.09.015\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKnödler M, Rühl C, Opdensteinen P, Buyel JF. Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study. \u003cem\u003eJ Vis Exp.\u003c\/em\u003e 2019;(150).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3791\/59933\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813630701866,"sku":"BTS-B2025400","price":1185.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025400.png?v=1790899968","url":"https:\/\/bluetigerscientific.com\/products\/low-density-glyoxal-4-fine-agarose-4-cross-linked","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}