{"product_id":"low-density-aminoethyl-agarose-6-cross-linked","title":"Low Density Aminoethyl Agarose (6% Cross-linked)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eLow Density Aminoethyl Agarose (6% Cross-linked) – Catalog #B2025424\u003c\/h2\u003e\n\u003cp\u003eLow Density Aminoethyl Agarose (Catalog #B2025424) is a weak anion-exchange chromatography resin composed of 6% cross-linked agarose beads functionalized with a low density of primary amino groups. The primary amine functional groups are positively charged at neutral pH, allowing the resin to bind and resolve negatively charged proteins. The \"low density\" designation indicates a reduced substitution level, which minimizes nonspecific interactions and is useful for applications where selectivity is more important than binding capacity.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025424\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAgarose composition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e6% cross-linked agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eFunctional group:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAminoethyl (−CH₂CH₂NH₂); low density\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCharge type:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eWeak anion exchange (positively charged)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAnion-exchange chromatography, protein purification, buffer exchange, polishing steps, fractionation of negatively charged biomolecules\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAminoethyl agarose, low density aminoethyl, weak anion exchanger, AE agarose, protein purification resin, ion exchange chromatography, 6BCL agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eIon exchange chromatography separates proteins based on their surface charge. Anion-exchange resins bind negatively charged molecules while allowing neutral or positively charged proteins to flow through. The primary amino group (pKa ~9) carries a positive charge above pH 5, making aminoethyl-functionalized agarose a weak anion exchanger useful across a wide pH range. Low-density resins reduce nonspecific hydrophobic interactions and are often preferred when separation resolution is more critical than maximum binding capacity—for instance, when purifying similar proteins or performing polishing steps after earlier chromatography.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eAnion-exchange chromatography and protein fractionation\u003c\/li\u003e\n\u003cli\u003eBuffer exchange and desalting of protein solutions\u003c\/li\u003e\n\u003cli\u003ePolishing and intermediate purification steps\u003c\/li\u003e\n\u003cli\u003eSelective separation of acidic proteins and nucleic acids\u003c\/li\u003e\n\u003cli\u003eGentle separation conditions to preserve protein integrity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the suspension at 2–8°C. Before use, gently resuspend and allow the resin to settle to assess the bed height. Pack the column according to standard practice (gravity flow or pump) and equilibrate thoroughly with the starting buffer. Optimize pH and salt concentration empirically for your protein pair; typical working pH is 7–9.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBuffer pH:\u003c\/strong\u003e Effective from pH 5 to \u0026gt;9; the effective range depends on the pKa of your protein and the resin charge.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIonic strength:\u003c\/strong\u003e Begin with low salt (e.g., 20 mM Tris, pH 8.0) and increase salt in a gradient to elute bound protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLoading:\u003c\/strong\u003e Start conservatively; the low density limits maximum capacity, so optimize for selectivity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegeneration:\u003c\/strong\u003e Wash with high-salt buffer (e.g., 1 M NaCl) to strip protein, then re-equilibrate before the next run.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 mL of low-density aminoethyl agarose suspension (6% cross-linked)\u003c\/li\u003e\n\u003cli\u003eWeak anion-exchange resin suitable for selectivity-focused applications\u003c\/li\u003e\n\u003cli\u003eEnough material for multiple purification experiments\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eLow charge density for higher selectivity and lower nonspecific adsorption\u003c\/li\u003e\n\u003cli\u003eGentle conditions suitable for sensitive proteins and regulatory molecules\u003c\/li\u003e\n\u003cli\u003eWide effective pH range (pH 5–\u0026gt;9)\u003c\/li\u003e\n\u003cli\u003eCost-effective for development and polishing steps\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between low density and standard density aminoethyl agarose?\u003c\/strong\u003e\u003cbr\u003eLow-density resin has fewer amino groups per unit volume, reducing nonspecific binding and improving selectivity, though with lower maximum binding capacity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat pH range should I use?\u003c\/strong\u003e\u003cbr\u003eAminoethyl is a weak anion exchanger with a pKa around 9. Effective separation typically occurs from pH 5 to \u0026gt;9, but optimize based on your protein's isoelectric point.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use this for cation exchange?\u003c\/strong\u003e\u003cbr\u003eNo. This resin is positively charged and binds negatively charged molecules. For cation exchange, use carboxymethyl (CM) or sulfopropyl (SP) resins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I pack a column with this resin?\u003c\/strong\u003e\u003cbr\u003eFollow standard chromatography packing procedures: allow the resin to fully suspend, pour into a column, and flow buffer through slowly until the bed height is stable.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a COA or technical datasheet?\u003c\/strong\u003e\u003cbr\u003eRequest a quote or contact us and we will provide available lot documentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLópez-Gallego F, Montes T, Fuentes M, Alonso N, Grazu V, Betancor L, Guisán JM, Fernández-Lafuente R. Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports. \u003cem\u003eJ Biotechnol.\u003c\/em\u003e 2005;116(1):1-10.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jbiotec.2004.09.015\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKnödler M, Rühl C, Opdensteinen P, Buyel JF. Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study. \u003cem\u003eJ Vis Exp.\u003c\/em\u003e 2019;(150).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3791\/59933\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eWilchek M, Miron T. Limitations of N-hydroxysuccinimide esters in affinity chromatography and protein immobilization. \u003cem\u003eBiochemistry.\u003c\/em\u003e 1987;26(8):2155-61.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/bi00382a014\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813631619370,"sku":"BTS-B2025424","price":1215.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025424.png?v=1790899987","url":"https:\/\/bluetigerscientific.com\/products\/low-density-aminoethyl-agarose-6-cross-linked","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}