{"product_id":"klh-conjugated-to-agarose-beads-2ml","title":"KLH Conjugated to Agarose Beads, 2 mL","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eKLH Conjugated to Agarose Beads – Catalog #B2025634\u003c\/h2\u003e\n\u003cp\u003eKLH Conjugated to Agarose Beads (Catalog #B2025634) combine keyhole limpet hemocyanin (KLH), a large (~400 kDa), highly immunogenic protein, with the practical advantages of agarose affinity matrix. This product is supplied as a 2 mL suspension of beads and is ideal for purifying antibodies specific to KLH-based immunogens or for negative selection in antibody screening workflows.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025634\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eKeyhole limpet (\u003cem\u003eMegathura crenulata\u003c\/em\u003e)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAntibody purification and screening, removal of anti-KLH antibodies, affinity-based protein separation, immunological assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eKeyhole limpet hemocyanin, KLH, KLH agarose beads, KLH-conjugated beads, KLH affinity beads, KLH-coated agarose, hemocyanin carrier protein, immunogenic KLH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eKeyhole limpet hemocyanin (KLH) is an oxygen-carrying metalloprotein from the keyhole limpet (\u003cem\u003eMegathura crenulata\u003c\/em\u003e) that has been widely used as a carrier protein for immunization. Because of its large size, multiple epitopes, and inherent immunogenicity, KLH conjugated to haptens or peptides is effective for raising high-titer, specific antibodies. Many research antibodies and commercial antibody kits have been developed using KLH conjugates as immunogens.\u003c\/p\u003e\n\u003cp\u003eWhen researchers generate antibodies against a KLH-conjugated antigen, some of the resulting antibodies recognize the target antigen, but others recognize KLH itself. KLH-conjugated agarose beads provide a simple method to remove or capture anti-KLH antibodies, either to negative-select for antibodies specific to the target hapten or peptide, or to enrich anti-KLH antibodies for analysis.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eDepletion of anti-KLH antibodies from hybridoma or polyclonal antibody preparations\u003c\/li\u003e\n\u003cli\u003ePurification of antibodies specific to KLH-conjugated antigens\u003c\/li\u003e\n\u003cli\u003eScreening of antibody libraries for KLH-binding species\u003c\/li\u003e\n\u003cli\u003eImmunological research and antibody characterization\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Before use, gently mix the suspension by inversion (do not vortex) and resuspend the beads. Protect from freeze–thaw cycles.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBatch binding:\u003c\/strong\u003e Add the beads to your antibody solution, incubate for 30 minutes to 1 hour at 4°C or room temperature, then separate by settling or brief centrifugation (1,000 × g for 1 minute).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColumn packing:\u003c\/strong\u003e Alternatively, pack the beads into a column for gravity-flow or low-pressure chromatography applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBuffer compatibility:\u003c\/strong\u003e Use antibody storage buffers (PBS, TBS, or similar); avoid extreme pH values that may destabilize KLH or agarose.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e2 mL of keyhole limpet hemocyanin conjugated to agarose beads, supplied as suspension\u003c\/li\u003e\n\u003cli\u003eA ready-to-use affinity matrix for antibody screening and purification\u003c\/li\u003e\n\u003cli\u003eHigh binding capacity for anti-KLH antibodies\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eKLH is a gold-standard immunogenic carrier widely used in antibody development\u003c\/li\u003e\n\u003cli\u003eAgarose support is chemically inert, biocompatible, and suitable for repeated use\u003c\/li\u003e\n\u003cli\u003eConvenient suspension format ready for immediate use in batch or column workflows\u003c\/li\u003e\n\u003cli\u003eEffective at depleting unwanted anti-KLH reactivity from antibody pools\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is KLH and why is it used as a carrier protein?\u003c\/strong\u003e\u003cbr\u003eKeyhole limpet hemocyanin is a large (~400 kDa), highly immunogenic oxygen-transport protein. Its size and multiple epitopes make it an excellent carrier for small antigens (haptens and peptides) to enhance immune recognition.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I use KLH beads to deplete anti-KLH antibodies?\u003c\/strong\u003e\u003cbr\u003eIncubate your antibody solution with the beads for 30 minutes to 1 hour at 4°C or room temperature, then separate the beads by settling or centrifugation. The flow-through or supernatant contains antibodies depleted of anti-KLH reactivity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse these beads?\u003c\/strong\u003e\u003cbr\u003eYes, with care. Gently wash the beads with PBS or your working buffer to remove unbound antibodies, and re-equilibrate before the next use. Repeated freeze–thaw cycles should be avoided.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat if I need to capture and enrich anti-KLH antibodies instead of depleting them?\u003c\/strong\u003e\u003cbr\u003eAfter incubation, elute the antibodies from the beads using a low-pH buffer (e.g., 0.1 M glycine, pH 2.5) or high-salt buffer, depending on the strength of binding you observe.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre there any monoclonal or recombinant alternatives to KLH-based products?\u003c\/strong\u003e\u003cbr\u003eYes. For some applications, protein G, protein A, or other affinity matrices may offer improved specificity. Contact us to discuss options for your specific experiment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHarris JR, Markl J. Keyhole limpet hemocyanin (KLH): a biomedical review. \u003cem\u003eMicron.\u003c\/em\u003e 1999;30(6):597-623.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0968-4328(99)00036-0\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eMontégut L, Chen H, Bravo-San Pedro JM, Motiño O, Martins I, Kroemer G. Immunization of mice with the self-peptide ACBP coupled to keyhole limpet hemocyanin. \u003cem\u003eSTAR Protoc.\u003c\/em\u003e 2022;3(1):101095.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.xpro.2021.101095\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLee BS, Huang JS, Jayathilaka LP, Lee J, Gupta S. Antibody Production with Synthetic Peptides. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2016;1474:25-47.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-4939-6352-2_2\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813633028394,"sku":"BTS-B2025634","price":2005.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025634.png?v=1790900025","url":"https:\/\/bluetigerscientific.com\/products\/klh-conjugated-to-agarose-beads-2ml","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}