{"product_id":"human-serum-proteins-conjugated-to-agarose-beads-5ml","title":"Human Serum Proteins Conjugated to Agarose Beads, 5 mL","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHuman Serum Proteins Conjugated to Agarose Beads, 5 mL – Catalog #B2025622\u003c\/h2\u003e\n\u003cp\u003eHuman Serum Proteins Conjugated to Agarose Beads (Catalog #B2025622) harnesses the natural diversity of human serum proteins immobilized on agarose beads. This complex mixture—including human serum albumin, immunoglobulins (IgG, IgA, IgM), transferrin, fibrinogen, and other plasma proteins—provides a broad-spectrum affinity matrix for protein separation, antibody isolation, and studies of protein-protein interactions. Supplied as 5 mL of suspension, it is particularly useful for affinity chromatography applications where natural serum composition and multi-protein interactions are beneficial.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025622\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity chromatography, protein purification, antibody selection and isolation, immunoaffinity separation, protein interaction studies, serum protein binding research\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman serum proteins, serum protein agarose, human proteins agarose beads, serum protein conjugate, human plasma proteins, agarose-bound serum proteins, serum protein affinity beads, human protein-agarose conjugate, immunoaffinity beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eHuman blood serum is a complex mixture of more than 4,000 distinct proteins with widely varying abundances, functions, and biochemical properties. The major components include human serum albumin (HSA, ~50% of total protein), immunoglobulins (IgG, IgA, IgM; ~20%), and numerous other plasma proteins such as transferrin, fibrinogen, complement components, protease inhibitors, and apolipoproteins. Each protein presents distinct binding sites, multiple domains, and diverse chemical functionalities.\u003c\/p\u003e\n\u003cp\u003eWhen conjugated to agarose beads, human serum proteins create a multi-faceted affinity support that captures molecules and proteins through a combination of:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eAlbumin's fatty acid and small-molecule binding sites\u003c\/li\u003e\n\u003cli\u003eImmunoglobulin-antigen interactions and antibody specificity\u003c\/li\u003e\n\u003cli\u003eComplement protein binding and immune complex formation\u003c\/li\u003e\n\u003cli\u003eGeneral protein-protein interactions and adsorptive forces\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eAffinity chromatography using native serum protein interactions\u003c\/li\u003e\n\u003cli\u003eAntibody selection, enrichment, and purification from complex mixtures\u003c\/li\u003e\n\u003cli\u003eSerum protein-binding research and ligand screening\u003c\/li\u003e\n\u003cli\u003eImmunoaffinity separations leveraging natural antibody specificity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Allow beads to warm to room temperature before use. Mix gently to resuspend; do not vortex vigorously. Avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eComplex composition:\u003c\/strong\u003e These beads contain multiple protein components, each with distinct binding properties. Binding specificity depends on pH, ionic strength, temperature, and ligand chemistry. Optimize conditions for your specific application.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEquilibration:\u003c\/strong\u003e Wash beads thoroughly with binding buffer before each use to remove contaminating proteins and ensure consistent performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding:\u003c\/strong\u003e Incubate sample with beads at 4°C for enhanced specificity; room temperature allows faster kinetics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Use standard low-pH buffers (0.1 M glycine pH 2.5, 0.1 M citrate pH 3), high-salt solutions (3 M NaCl), or organic solvent gradients depending on your target molecule; immediately neutralize pH after acidic elution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegeneration:\u003c\/strong\u003e Regenerate between cycles by washing with elution buffer and re-equilibrating with binding buffer.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of human serum proteins conjugated to agarose beads, supplied as a suspension\u003c\/li\u003e\n\u003cli\u003eA complex, multi-protein affinity support reflecting natural serum composition\u003c\/li\u003e\n\u003cli\u003eVersatile chromatography substrate for diverse protein interactions\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eReflects natural serum protein composition for physiologically relevant separations\u003c\/li\u003e\n\u003cli\u003eMultiple binding sites and protein components enable diverse separation strategies\u003c\/li\u003e\n\u003cli\u003eParticularly suited to antibody and immune-complex isolation applications\u003c\/li\u003e\n\u003cli\u003eCost-effective alternative to single-protein conjugates for exploratory chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow does this differ from HSA-alone or IgG-alone agarose beads?\u003c\/strong\u003e\u003cbr\u003eThese beads contain all major serum proteins, providing broader binding diversity and reflecting natural serum composition. Single-protein beads offer higher specificity for defined interactions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the protein composition of these beads?\u003c\/strong\u003e\u003cbr\u003eThe complex includes albumin, immunoglobulins (IgG, IgA, IgM), transferrin, fibrinogen, complement components, and other plasma proteins present in human serum. Precise composition varies by batch; request a COA\/TDS for details.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I optimize binding conditions for my specific application?\u003c\/strong\u003e\u003cbr\u003eStart with standard immunoaffinity chromatography conditions (neutral pH, low salt) and vary pH and ionic strength to identify the optimal selectivity for your target molecule.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I achieve high specificity with a multi-protein support?\u003c\/strong\u003e\u003cbr\u003eYes, if your target molecule has defined affinity for one or more of the serum proteins. Specificity is then determined by the specific protein interaction, not the overall support.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre lot-to-lot variations expected?\u003c\/strong\u003e\u003cbr\u003eAs a natural-product-derived reagent, some variation in composition and performance between lots is possible. Request comparative COA\/TDS data if consistency is critical for your application.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003ePark J, Kim MS, Park T, Kim YH, Shin DH. Crystal structure of pharmaceutical-grade human serum albumin. \u003cem\u003eInt J Biol Macromol.\u003c\/em\u003e 2021;166:221-228.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.ijbiomac.2020.10.152\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSalehi N, Peng CA. Purification of CD47-streptavidin fusion protein from bacterial lysate using biotin-agarose affinity chromatography. \u003cem\u003eBiotechnol Prog.\u003c\/em\u003e 2016;32(4):949-58.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/btpr.2293\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGrodzki AC, Berenstein E. Antibody purification: affinity chromatography - protein A and protein G Sepharose. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2010;588:33-41.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-59745-324-0_5\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813632340266,"sku":"BTS-B2025622","price":1175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025622.png?v=1790900002","url":"https:\/\/bluetigerscientific.com\/products\/human-serum-proteins-conjugated-to-agarose-beads-5ml","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}