{"product_id":"human-serum-albumin-conjugated-to-agarose-beads-high-capacity-1","title":"Human Serum Albumin Conjugated to Agarose Beads, 5 mL, High-Capacity","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHuman Serum Albumin Conjugated to Agarose Beads, High-Capacity, 5 mL – Catalog #B2025631\u003c\/h2\u003e\n\u003cp\u003eHuman Serum Albumin Conjugated to Agarose Beads – High-Capacity (Catalog #B2025631) is a premium-grade formulation of HSA immobilized on agarose at elevated density, optimized for applications requiring maximum binding capacity and throughput. Supplied as 5 mL of suspension, this conjugate is designed for large-scale protein purification, pharmaceutical screening, and bioaffinity chromatography where sample volume or antigen concentration demands efficient, high-capacity affinity separation. The globular 66 kDa HSA structure features three major binding domains with numerous pockets for small molecules and proteins.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025631\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHigh-throughput protein purification, large-scale affinity chromatography, drug and small-molecule screening, pharmaceutical discovery, bioaffinity applications, protein separation from complex matrices\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman serum albumin, HSA, high-capacity HSA agarose, high-concentration HSA beads, serum albumin agarose, HSA affinity beads, agarose-immobilized albumin, albumin-coupled agarose, large-scale protein purification, high-capacity agarose beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eHuman Serum Albumin (HSA) is the most abundant protein in blood plasma, comprising approximately 50–60% of total plasma proteins. Its 66 kDa globular structure is composed of three homologous domains (I, II, and III), each containing distinct ligand-binding sites. HSA is an exceptional transporter and binder of hydrophobic molecules, including fatty acids (up to ~10 molecules per HSA), hormones, bilirubin, and pharmaceuticals. These multivalent binding properties make HSA an ideal affinity ligand for chromatographic separations and bioanalytical research.\u003c\/p\u003e\n\u003cp\u003eThe high-capacity formulation delivers increased HSA loading density per unit of agarose beads, enabling:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eEnhanced binding capacity for large sample volumes\u003c\/li\u003e\n\u003cli\u003eFaster binding kinetics due to higher HSA concentration\u003c\/li\u003e\n\u003cli\u003eGreater efficiency in high-throughput applications\u003c\/li\u003e\n\u003cli\u003eImproved cost-effectiveness for large-scale processing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eLarge-scale affinity chromatography for HSA-ligand enrichment\u003c\/li\u003e\n\u003cli\u003ePurification of abundant HSA-binding proteins or antibodies\u003c\/li\u003e\n\u003cli\u003eHigh-throughput pharmaceutical screening and drug discovery\u003c\/li\u003e\n\u003cli\u003eProcessing of concentrated samples or crude extracts\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Allow beads to warm to room temperature before use. Mix gently to resuspend beads; vigorous vortexing may cause bead aggregation. Avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh-capacity workflows:\u003c\/strong\u003e The elevated HSA density supports larger sample volumes and higher antigen concentrations; pre-titrate to determine the optimal bead-to-sample ratio.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding conditions:\u003c\/strong\u003e Incubate at 4°C for maximum selectivity; room temperature permits faster kinetics. Equilibrate beads thoroughly with binding buffer before each use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Standard protocols use 0.1 M glycine pH 2.5, 3 M NaCl, or pH 8–9 buffers; pH neutralization must follow acidic elution immediately.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegeneration:\u003c\/strong\u003e Beads support multiple cycles of regeneration and reuse; capacity and binding kinetics remain stable over 10+ cycles with proper technique.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of high-capacity human serum albumin conjugated to agarose beads, supplied as a suspension\u003c\/li\u003e\n\u003cli\u003ePremium-grade affinity support with enhanced binding density\u003c\/li\u003e\n\u003cli\u003eOptimized for large-scale and high-throughput applications\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh HSA density enables efficient separation of abundant HSA-binding targets\u003c\/li\u003e\n\u003cli\u003eIncreased binding capacity reduces beads required per reaction, lowering per-assay costs\u003c\/li\u003e\n\u003cli\u003eWell-established HSA structure and binding properties enable rational experimental design\u003c\/li\u003e\n\u003cli\u003eCompatible with standard and automated chromatography systems for high-throughput work\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between standard and high-capacity HSA agarose beads?\u003c\/strong\u003e\u003cbr\u003eHigh-capacity formulations feature elevated HSA loading density, providing greater binding capacity per unit of beads. Use high-capacity for larger sample volumes, higher antigen concentrations, or high-throughput applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow many ligand-binding sites does HSA have?\u003c\/strong\u003e\u003cbr\u003eHSA contains ~10 high-affinity sites for fatty acids and multiple lower-affinity pockets for diverse small molecules. Capacity also depends on conjugation density and your specific ligand.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan these beads handle concentrated protein samples?\u003c\/strong\u003e\u003cbr\u003eYes. The high-capacity formulation is designed for concentrated samples. Pre-titrate with your sample to find optimal bead:sample ratio to avoid saturation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is typical binding capacity per mL of beads?\u003c\/strong\u003e\u003cbr\u003eCapacity varies with HSA loading density and the specific ligand. Request a COA\/TDS for lot-specific capacity data.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre these beads compatible with automated systems?\u003c\/strong\u003e\u003cbr\u003eYes. The suspension format works with standard and many automated liquid handlers. Confirm compatibility with your specific equipment before large-scale implementation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003ePark J, Kim MS, Park T, Kim YH, Shin DH. Crystal structure of pharmaceutical-grade human serum albumin. \u003cem\u003eInt J Biol Macromol.\u003c\/em\u003e 2021;166:221-228.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.ijbiomac.2020.10.152\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eCatalano C, Lucier KW, To D, Senko S, Tran NL, Farwell AC, Silva SM, Dip PV, Poweleit N, Scapin G. The CryoEM structure of human serum albumin in complex with ligands. \u003cem\u003eJ Struct Biol.\u003c\/em\u003e 2024;216(3):108105.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jsb.2024.108105\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSalehi N, Peng CA. Purification of CD47-streptavidin fusion protein from bacterial lysate using biotin-agarose affinity chromatography. \u003cem\u003eBiotechnol Prog.\u003c\/em\u003e 2016;32(4):949-58.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/btpr.2293\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813632897322,"sku":"BTS-B2025631","price":1765.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025631_97262a95-5511-4fac-b08c-5146f3afe31b.png?v=1790900021","url":"https:\/\/bluetigerscientific.com\/products\/human-serum-albumin-conjugated-to-agarose-beads-high-capacity-1","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}