{"product_id":"human-serum-albumin-conjugated-to-agarose-beads-5ml","title":"Human Serum Albumin Conjugated to Agarose Beads, 5 mL","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHuman Serum Albumin Conjugated to Agarose Beads, 5 mL – Catalog #B2025623\u003c\/h2\u003e\n\u003cp\u003eHuman Serum Albumin Conjugated to Agarose Beads (Catalog #B2025623) combines the multifunctional protein-binding properties of human serum albumin (HSA) with the inert, hydrophilic agarose matrix. Supplied as 5 mL of suspension, this conjugate provides a versatile affinity support for protein purification, ligand binding studies, and bioaffinity chromatography. HSA's 66 kDa globular structure contains numerous binding sites for fatty acids, pharmaceuticals, and other small molecules, as well as non-specific protein interactions useful for affinity separation applications.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025623\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity chromatography, protein purification, fatty acid and drug binding studies, bioaffinity applications, protein separation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman serum albumin, HSA, serum albumin agarose, HSA agarose beads, albumin conjugated agarose, human albumin affinity beads, agarose-immobilized albumin, albumin-coupled agarose, protein purification beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eHuman Serum Albumin (HSA) is a major blood plasma protein (~66 kDa) produced by the liver. Its structure consists of three homologous domains, each with multiple ligand-binding pockets. HSA serves as a transport protein for fatty acids, hormones, pharmaceuticals, and other hydrophobic molecules in blood, and its multivalent binding properties make it a valuable tool in affinity chromatography and bioanalytical research.\u003c\/p\u003e\n\u003cp\u003eWhen conjugated to agarose beads, HSA creates a versatile affinity matrix useful for:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eCapturing HSA-binding molecules (fatty acids, drugs, bilirubin)\u003c\/li\u003e\n\u003cli\u003ePurifying HSA-binding proteins or antibodies\u003c\/li\u003e\n\u003cli\u003eGeneral protein separation based on affinity interactions\u003c\/li\u003e\n\u003cli\u003eStudying HSA-ligand interactions and binding kinetics\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eAffinity chromatography for HSA-ligand enrichment\u003c\/li\u003e\n\u003cli\u003ePurification of HSA-binding proteins or antibodies\u003c\/li\u003e\n\u003cli\u003eDrug and small-molecule compound screening\u003c\/li\u003e\n\u003cli\u003eBioaffinity applications requiring a universal protein carrier\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Allow beads to warm to room temperature before use. Mix gently to resuspend beads; do not vortex. Avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eEquilibration:\u003c\/strong\u003e Wash beads thoroughly with binding buffer (PBS, TBS, or custom buffer) before each use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding:\u003c\/strong\u003e Incubate sample with beads at 4°C for enhanced specificity or room temperature for faster kinetics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Use low-pH buffers (0.1 M glycine pH 2.5) or high-salt solutions (3 M NaCl), followed by immediate pH neutralization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegeneration:\u003c\/strong\u003e Beads can be regenerated using standard washing and re-equilibration protocols for multiple reuse cycles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of human serum albumin conjugated to agarose beads, supplied as a suspension\u003c\/li\u003e\n\u003cli\u003eA versatile affinity support with multiple ligand-binding sites\u003c\/li\u003e\n\u003cli\u003eSuitable for affinity chromatography and bioaffinity research\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHSA's multiple binding sites enable diverse separation and purification applications\u003c\/li\u003e\n\u003cli\u003eWell-characterized structure and binding properties backed by extensive literature\u003c\/li\u003e\n\u003cli\u003eCompatible with standard chromatography workflows and equipment\u003c\/li\u003e\n\u003cli\u003eCost-effective carrier protein for affinity-based research and drug screening\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat can I bind to HSA agarose beads?\u003c\/strong\u003e\u003cbr\u003eHSA binds many small molecules (fatty acids, drugs, hormones), as well as proteins with affinity for albumin. Specificity depends on binding pocket chemistry and buffer conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow is HSA-agarose different from protein A or protein G?\u003c\/strong\u003e\u003cbr\u003eHSA is a general carrier protein with broad ligand-binding properties, while protein A and G are highly specific for the Fc region of antibodies. HSA is preferred for non-antibody applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat elution pH and salt are recommended?\u003c\/strong\u003e\u003cbr\u003eStandard protocols use 0.1 M glycine pH 2.5, 3 M NaCl, or pH 8–9 buffers depending on ligand stability. Optimize for your specific application.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads for multiple cycles?\u003c\/strong\u003e\u003cbr\u003eYes. Regenerate by washing with elution buffer and re-equilibrating with binding buffer. Beads typically support 5–10+ reuse cycles with proper care.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre there lot-specific binding capacity data available?\u003c\/strong\u003e\u003cbr\u003eYes. Request a COA\/TDS for capacity information for your specific lot; binding capacity varies with HSA loading density.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003ePark J, Kim MS, Park T, Kim YH, Shin DH. Crystal structure of pharmaceutical-grade human serum albumin. \u003cem\u003eInt J Biol Macromol.\u003c\/em\u003e 2021;166:221-228.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.ijbiomac.2020.10.152\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eChubarov A, Spitsyna A, Krumkacheva O, Mitin D, Suvorov D, Tormyshev V, Fedin M, Bowman MK, Bagryanskaya E. Reversible Dimerization of Human Serum Albumin. \u003cem\u003eMolecules.\u003c\/em\u003e 2020;26(1).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3390\/molecules26010108\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSalehi N, Peng CA. Purification of CD47-streptavidin fusion protein from bacterial lysate using biotin-agarose affinity chromatography. \u003cem\u003eBiotechnol Prog.\u003c\/em\u003e 2016;32(4):949-58.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/btpr.2293\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813632274730,"sku":"BTS-B2025623","price":1175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025623.png?v=1790900001","url":"https:\/\/bluetigerscientific.com\/products\/human-serum-albumin-conjugated-to-agarose-beads-5ml","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}