{"product_id":"human-igm-conjugated-to-agarose-beads-high-concentration","title":"Human IgM Conjugated to Agarose Beads, 2 mL, High-Concentration","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHuman IgM Conjugated to Agarose Beads, High-Concentration, 2 mL – Catalog #B2025629\u003c\/h2\u003e\n\u003cp\u003eHuman IgM Conjugated to Agarose Beads – High-Concentration (Catalog #B2025629) is a high-density immobilized form of human Immunoglobulin M providing enhanced capacity for antigen capture and immune complex isolation. Supplied as 2 mL of premium-grade suspension, this conjugate is optimized for applications requiring maximum binding capacity and multi-valent interactions. The pentameric structure of IgM (approximately 900 kDa) with five Fab domains ensures simultaneous engagement of multiple epitopes, improving both sensitivity and specificity in immunological research.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025629\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHigh-capacity affinity chromatography, antigen capture, immunoprecipitation, immune complex isolation, antibody enrichment, flow cytometry applications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman IgM agarose beads, IgM conjugated agarose, high-concentration IgM, immunoglobulin M affinity beads, human antibody agarose, IgM-agarose conjugate, human IgM affinity beads, agarose-immobilized IgM, IgM coupled agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eImmunoglobulin M (IgM) is the largest antibody isotype, composed of five identical monoclonal units (each ~50 kDa Fab domain) held together by a J chain, yielding a total molecular weight of approximately 900 kDa. Its pentameric architecture gives IgM exceptional avidity and the ability to cross-link multiple antigens. IgM is the first antibody produced in primary immune responses and is a potent activator of the classical complement pathway, making it a valuable research reagent for detecting and capturing diverse antigens and immune complexes.\u003c\/p\u003e\n\u003cp\u003eThe high-concentration formulation of this product delivers increased IgM density per unit of agarose beads, enabling enhanced binding capacity and faster kinetics for applications demanding high-throughput processing or large-volume sample handling.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh-capacity affinity chromatography for antigen purification from crude extracts\u003c\/li\u003e\n\u003cli\u003eImmunoprecipitation of abundant antigens and immune complexes\u003c\/li\u003e\n\u003cli\u003eComplement-mediated separation and concentration of target antigens\u003c\/li\u003e\n\u003cli\u003eFlow cytometry sample preparation and cell enrichment\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Allow beads to warm to room temperature before use. Mix gently to resuspend beads; do not vortex vigorously. Avoid repeated freeze–thaw cycles, which may reduce capacity.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh-capacity protocols:\u003c\/strong\u003e The increased IgM density supports larger sample volumes and higher antigen concentrations; pre-titrate with your specific antigen to determine optimal bead:antigen ratio.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding conditions:\u003c\/strong\u003e Incubate at 4°C for maximum specificity or room temperature for increased kinetics; higher IgM density reduces incubation time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Use standard protocols (0.1 M glycine pH 2.5, 3 M NaCl, or other low-pH conditions) followed by immediate pH neutralization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegeneration:\u003c\/strong\u003e Beads can be regenerated and reused multiple times; capacity is retained over several cycles with proper care.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e2 mL of high-concentration human IgM conjugated to agarose beads, supplied as a suspension\u003c\/li\u003e\n\u003cli\u003ePremium-grade affinity support with enhanced binding capacity\u003c\/li\u003e\n\u003cli\u003eOptimized for high-throughput and large-scale antigen capture protocols\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh IgM density for maximum binding capacity and faster kinetics\u003c\/li\u003e\n\u003cli\u003ePentameric structure ensures high avidity and multi-point antigen engagement\u003c\/li\u003e\n\u003cli\u003eEfficient complement activation useful in specialized immunological assays\u003c\/li\u003e\n\u003cli\u003eSuitable for both batch and column-based chromatography workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhy choose high-concentration over standard IgM agarose beads?\u003c\/strong\u003e\u003cbr\u003eHigh-concentration formulations enable faster reactions, require fewer beads per reaction, and are ideal for high-throughput screening or processing large sample volumes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow does the pentameric structure improve antigen capture?\u003c\/strong\u003e\u003cbr\u003eEach IgM molecule has five Fab domains, allowing simultaneous binding to multiple epitopes on a single antigen or cross-linking of multiple antigens, increasing both avidity and sensitivity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the expected binding capacity?\u003c\/strong\u003e\u003cbr\u003eCapacity depends on antigen size, concentration, and binding conditions; request a COA\/TDS or contact us for lot-specific binding capacity data.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan these beads be used with automated systems?\u003c\/strong\u003e\u003cbr\u003eYes. The suspension format is compatible with standard pipetting and many automated liquid handlers; confirm specifications with us if using specialized equipment.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow many times can I regenerate these beads?\u003c\/strong\u003e\u003cbr\u003eBeads typically support multiple reuse cycles (5–10 or more) with proper care and appropriate elution buffers; capacity may diminish slightly with each cycle.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eYang S, Zhao LC, Guo FZ, Xu HS, Huang MM, Fei H. Evolution of immunoglobulin M (IgM) structure and function in vertebrates: A comprehensive review. \u003cem\u003eFish Shellfish Immunol.\u003c\/em\u003e 2025;166:110635.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.fsi.2025.110635\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBuchner J, Sitia R, Svilenov HL. Understanding IgM Structure and Biology to Engineer New Antibody Therapeutics. \u003cem\u003eBioDrugs.\u003c\/em\u003e 2025;39(3):347-357.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/s40259-025-00720-6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSalehi N, Peng CA. Purification of CD47-streptavidin fusion protein from bacterial lysate using biotin-agarose affinity chromatography. \u003cem\u003eBiotechnol Prog.\u003c\/em\u003e 2016;32(4):949-58.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/btpr.2293\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813632635178,"sku":"BTS-B2025629","price":2005.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025629.png?v=1790900012","url":"https:\/\/bluetigerscientific.com\/products\/human-igm-conjugated-to-agarose-beads-high-concentration","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}