{"product_id":"human-igm-conjugated-to-agarose-beads-2ml","title":"Human IgM Conjugated to Agarose Beads, 2 mL","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHuman IgM Conjugated to Agarose Beads, 2 mL – Catalog #B2025621\u003c\/h2\u003e\n\u003cp\u003eHuman IgM Conjugated to Agarose Beads (Catalog #B2025621) combines the immunological specificity of human Immunoglobulin M with the solid support matrix of agarose beads. Supplied as 2 mL of suspension, this conjugate provides a convenient substrate for affinity chromatography, antigen capture, and immunoprecipitation protocols. IgM antibodies are ideal for detecting multiple antigens simultaneously due to their pentameric structure and high avidity.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025621\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity chromatography, antigen capture, immunoprecipitation, immune complex isolation, antibody purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman IgM agarose beads, IgM conjugated agarose, immunoglobulin M affinity beads, human antibody agarose, IgM-agarose conjugate, human IgM affinity beads, agarose-immobilized IgM, IgM coupled agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eImmunoglobulin M (IgM) is a pentameric antibody composed of five Fab units joined by a J chain, making it the largest antibody with a molecular weight of approximately 900 kDa. IgM is the first antibody produced during the primary immune response and is particularly effective at complement activation and agglutination of antigens. Its large size and multiple binding sites provide high avidity and the ability to cross-link multiple antigens simultaneously, making it a powerful tool for immunological research.\u003c\/p\u003e\n\u003cp\u003eAgarose is an inert, hydrophilic polysaccharide polymer ideal for affinity chromatography and protein purification. When human IgM is conjugated to agarose beads, it creates a stable, reusable affinity support for capturing specific antigens and immune complexes from complex mixtures.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eAffinity chromatography for antigen capture and purification\u003c\/li\u003e\n\u003cli\u003eImmunoprecipitation of specific antigens and immune complexes\u003c\/li\u003e\n\u003cli\u003eIsolation of complement-bound antigens\u003c\/li\u003e\n\u003cli\u003eAntibody purification and selection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Allow beads to warm to room temperature before use. Mix gently to resuspend beads; do not vortex. Avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eEquilibration:\u003c\/strong\u003e Wash beads thoroughly with binding buffer before each use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding:\u003c\/strong\u003e Incubate sample with beads at 4°C for enhanced specificity, or room temperature for faster kinetics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Use standard immunoglobulin elution conditions (low pH buffer, 0.1 M glycine pH 2.5, or high salt) followed by immediate pH neutralization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegeneration:\u003c\/strong\u003e Beads can be regenerated multiple times using appropriate washing and re-equilibration buffers.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e2 mL of human IgM conjugated to agarose beads, supplied as a suspension\u003c\/li\u003e\n\u003cli\u003eReady-to-use affinity support for antigen capture and purification\u003c\/li\u003e\n\u003cli\u003eStable, reusable affinity matrix for multiple cycles\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003ePentameric structure and high avidity for sensitive antigen detection and capture\u003c\/li\u003e\n\u003cli\u003eEffective at complement activation, useful in immunological studies\u003c\/li\u003e\n\u003cli\u003eInert agarose matrix suitable for both batch and column chromatography\u003c\/li\u003e\n\u003cli\u003eEasy handling and magnetic separation compatibility with standard lab workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between IgG and IgM?\u003c\/strong\u003e\u003cbr\u003eIgG is a monomeric antibody present in secondary immune responses, while IgM is a large pentameric antibody produced first in the primary response. IgM has higher avidity and is more efficient at complement activation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I regenerate these beads?\u003c\/strong\u003e\u003cbr\u003eYes. After elution, wash thoroughly with elution buffer and re-equilibrate with binding buffer. Beads can typically be used for multiple cycles, though binding capacity may decrease over time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat buffers should I use for binding and elution?\u003c\/strong\u003e\u003cbr\u003eBinding buffers typically include PBS, TBS, or custom buffers. For elution, standard protocols use 0.1 M glycine pH 2.5, 3 M NaCl, or other low-pH buffers depending on your antigen stability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I prevent non-specific binding?\u003c\/strong\u003e\u003cbr\u003ePre-block beads with BSA or other carrier proteins, and optimize salt concentration and pH in your binding buffer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these with magnetic separation equipment?\u003c\/strong\u003e\u003cbr\u003eConfirm with us whether your batch is magnetic; some formulations are available with magnetic cores for convenient separation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eYang S, Zhao LC, Guo FZ, Xu HS, Huang MM, Fei H. Evolution of immunoglobulin M (IgM) structure and function in vertebrates: A comprehensive review. \u003cem\u003eFish Shellfish Immunol.\u003c\/em\u003e 2025;166:110635.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.fsi.2025.110635\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBuchner J, Sitia R, Svilenov HL. Understanding IgM Structure and Biology to Engineer New Antibody Therapeutics. \u003cem\u003eBioDrugs.\u003c\/em\u003e 2025;39(3):347-357.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/s40259-025-00720-6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSalehi N, Peng CA. Purification of CD47-streptavidin fusion protein from bacterial lysate using biotin-agarose affinity chromatography. \u003cem\u003eBiotechnol Prog.\u003c\/em\u003e 2016;32(4):949-58.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/btpr.2293\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813632078122,"sku":"BTS-B2025621","price":1295.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025621.png?v=1790899996","url":"https:\/\/bluetigerscientific.com\/products\/human-igm-conjugated-to-agarose-beads-2ml","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}