{"product_id":"human-igg-conjugated-to-agarose-beads-2-ml-high-capacity","title":"Human IgG Conjugated to Agarose Beads, 2 mL (High-Capacity)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHuman IgG Conjugated to Agarose Beads, 2 mL (High-Capacity) – Catalog #B2025628\u003c\/h2\u003e\n\u003cp\u003eHuman IgG Conjugated to Agarose Beads, High-Capacity (Catalog #B2025628) consist of human immunoglobulin G (IgG) antibodies at enhanced density immobilized on agarose beads, supplied as 2 mL of suspension. The agarose matrix provides a stable, inert support with large pore size and high protein binding capacity. The enhanced ligand density of this version enables capture of larger amounts of target antigens, epitopes, and proteins that bind human antibodies, making it suitable for large-scale purification and production-scale applications.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025628\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eImmobilized ligand:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman immunoglobulin G (IgG), high-capacity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead matrix:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAgarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLarge-scale affinity purification, production-scale antibody-based separation, high-volume immunoaffinity chromatography, extensive protein purification, bioprocess-scale applications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHuman IgG agarose beads high-capacity, human IgG conjugated agarose high-density, immobilized human antibodies, IgG affinity beads, human immunoglobulin G agarose, agarose-bound human IgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eAgarose-based affinity chromatography materials have become standard tools for rapid, high-yield purification of proteins and other biomolecules. The agarose polysaccharide matrix is chemically inert, supports large pore sizes, and tolerates a wide range of pH and ionic strength conditions. When human immunoglobulin G (IgG) is covalently immobilized onto agarose beads at high density, the resulting material provides increased binding capacity for antigens, epitopes, and proteins that bind human IgG, enabling straightforward affinity separation at production scales. The high-capacity version is optimized for applications requiring capture of larger amounts of target protein or extended use before saturation. The beads can be used in column formats, batch binding, or with automated liquid handlers for rapid sample preparation in proteomics, bioprocess development, and large-scale purification applications.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eLarge-scale affinity purification of antigens and epitopes from high-volume samples\u003c\/li\u003e\n\u003cli\u003eProduction-scale enrichment of human IgG-binding proteins\u003c\/li\u003e\n\u003cli\u003eBioprocess-scale development and manufacturing applications\u003c\/li\u003e\n\u003cli\u003eExtended use protocols requiring high binding capacity and minimal resin replacement\u003c\/li\u003e\n\u003cli\u003eDepletion of large amounts of human IgG-binding contaminants from samples\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the suspension at 2–8°C. Mix gently before use. The beads can be used in column, batch, or automated formats depending on your application.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding:\u003c\/strong\u003e Add beads to your sample and incubate at appropriate pH and temperature to allow human IgG to capture target antigens or proteins; optimize binding conditions for your specific application and scale.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeparation:\u003c\/strong\u003e Centrifuge to collect beads, then remove supernatant. Alternatively, use in column flow-through mode for larger-scale applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Resuspend and wash beads with appropriate buffer to remove unbound material and improve purity of bound complexes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Elute bound proteins by lowering pH, increasing salt concentration, or using competitive displacement, depending on binding strength and your downstream requirements. Request the COA\/TDS for lot-specific elution protocols and capacity data.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e2 mL of high-capacity human IgG-conjugated agarose bead suspension\u003c\/li\u003e\n\u003cli\u003eEnhanced ligand density for larger-scale applications\u003c\/li\u003e\n\u003cli\u003eReady-to-use affinity chromatography resin optimized for production-scale work\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh-capacity ligand density allows capture of larger amounts of target protein\u003c\/li\u003e\n\u003cli\u003eAgarose matrix is inert, chemically stable, and compatible with standard chromatography equipment and automation\u003c\/li\u003e\n\u003cli\u003eSimple protocol scales from bench to production: mix, bind, wash, and elute\u003c\/li\u003e\n\u003cli\u003eIdeal for bioprocess development and manufacturing-scale applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat proteins can I purify with these beads?\u003c\/strong\u003e\u003cbr\u003eThese beads capture antigens, epitopes, and proteins that bind human immunoglobulin G. The high-capacity version is optimized for large-volume samples and extended binding protocols. Optimize your binding conditions for your specific target.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between the standard and high-capacity versions?\u003c\/strong\u003e\u003cbr\u003eThe high-capacity version (B2025628) has enhanced IgG ligand density compared to the standard version (B2025620), providing higher binding capacity per unit volume. Choose the high-capacity version for large-scale purifications or bioprocess applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I elute bound material?\u003c\/strong\u003e\u003cbr\u003eElution depends on the strength of the IgG-antigen interaction. Try pH changes (0.1 M glycine–HCl, pH 2.5–3.0), increased salt, or mild detergents. Request the COA or contact us for optimization guidance and capacity data.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse these beads?\u003c\/strong\u003e\u003cbr\u003eYes. After elution, regenerate beads by washing with neutral buffer and storing at 2–8°C. Reusability depends on the strength of your elution conditions; request lot documentation for regeneration protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I store them?\u003c\/strong\u003e\u003cbr\u003eKeep at 2–8°C. Gently mix before use to ensure even suspension.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a COA or technical data?\u003c\/strong\u003e\u003cbr\u003eYes. Request a quote or contact us and we will provide available lot documentation including ligand density, binding capacity, and performance specifications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGrodzki AC, Berenstein E. Antibody purification: affinity chromatography - protein A and protein G Sepharose. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2010;588:33-41.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-59745-324-0_5\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBlank K, Lindner P, Diefenbach B, Plückthun A. Self-immobilizing recombinant antibody fragments for immunoaffinity chromatography: generic, parallel, and scalable protein purification. \u003cem\u003eProtein Expr Purif.\u003c\/em\u003e 2002;24(2):313-22.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1006\/prep.2001.1575\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBabashak JV, Phillips TM. Isolation of a specific membrane protein by immunoaffinity chromatography with biotinylated antibodies immobilized on avidin-coated glass beads. \u003cem\u003eJ Chromatogr.\u003c\/em\u003e 1989;476:187-94.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0021-9673(01)93868-3\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813632995626,"sku":"BTS-B2025628","price":2005.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025628.png?v=1790900025","url":"https:\/\/bluetigerscientific.com\/products\/human-igg-conjugated-to-agarose-beads-2-ml-high-capacity","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}