{"product_id":"high-density-metal-free-resin","title":"High Density Metal Free Resin","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHigh Density Metal Free Resin – Catalog #B2025392\u003c\/h2\u003e\n\u003cp\u003eHigh Density Metal Free Resin (Catalog #B2025392) is a purified agarose chromatography matrix manufactured and handled to eliminate trace metal contamination. Metal ions (particularly iron, copper, and zinc) can interfere with sensitive biochemical assays, chelation studies, cell culture, and metal-dependent enzyme kinetics. This metal-free formulation is supplied as 500 mL of high-density resin, ready for use in any affinity chromatography, ion exchange, or size-exclusion application where metal contamination must be minimized. The large volume makes it well-suited for process-scale purification and development work.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025392\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMatrix composition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHigh-density agarose (metal-free grade)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMetal content:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMinimized; specific trace metal limits available upon request\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMetal-sensitive protein purification, chelation studies, metal-free chromatography, cell culture work, enzymatic assays with metal-dependent reactions, in vitro translations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMetal-free resin, metal-free agarose, non-metallic chromatography matrix, purified agarose, low-metal resin, trace metal-free support, metal-free affinity resin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eTrace metals in chromatography resins and buffers can have profound effects on sensitive biochemical experiments. Iron and copper are redox-active and can participate in free-radical reactions; zinc and nickel are often cofactors in metalloenzymes or ligands in chelation studies; magnesium and calcium influence protein aggregation and enzyme kinetics. Even subnanomolar concentrations of transition metals can compromise metal-chelation affinity chromatography, inhibit metal-sensitive phosphatases and kinases, or interfere with colorimetric assays and spectrophotometric kinetics.\u003c\/p\u003e\n\u003cp\u003eThis metal-free resin is manufactured and handled using metal-free buffers and containers to reduce trace metal pickup from manufacturing, storage, and handling. The high density of the agarose matrix provides good flow characteristics and mechanical stability while maintaining the low-metal composition. It is compatible with all standard chromatography techniques (affinity, ion exchange, size exclusion, hydrophobic interaction) and can be derivatized or functionalized like standard agarose, provided the modifications do not reintroduce metals.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003ePurification of metalloproteins and metal-binding proteins where contamination would distort binding studies\u003c\/li\u003e\n\u003cli\u003eChelation chromatography and metal-affinity purification (IMAC) where background metal must be controlled\u003c\/li\u003e\n\u003cli\u003eIn vitro protein synthesis and translation systems sensitive to metal inhibition\u003c\/li\u003e\n\u003cli\u003eCell culture supplement (as a purified, metal-free matrix in bioreactor media)\u003c\/li\u003e\n\u003cli\u003eMetal-sensitive enzymatic assays (kinases, phosphatases, proteases dependent on specific cofactors)\u003c\/li\u003e\n\u003cli\u003eSpectrophotometric assays where trace metals cause background absorbance or fluorescence\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eTo maintain metal-free status, use only metal-free or chelated buffers (e.g., prepared with EDTA-treated water or purified through metal-free resin) and avoid metal-containing equipment. Prepare buffers in plastic containers, not glassware that may have been in contact with metal-containing reagents. Store at 2–8°C in sealed, metal-free containers. If functionalization is required, use metal-free coupling reagents and buffers.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBuffer preparation:\u003c\/strong\u003e Use Type I ultrapure water (\u0026gt;18 MΩ-cm resistivity) treated to remove trace metals. Pre-treat buffers through a column of metal-free resin or prepare with EDTA and chelex as needed.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEquipment:\u003c\/strong\u003e Use plastic or polytetrafluoroethylene (PTFE) columns; avoid glass or metal fittings. Polypropylene tubes are preferred for storage and handling.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTrace metal measurement:\u003c\/strong\u003e If your application requires known metal limits, request ICP-MS analysis of your lot (available upon request).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResin decontamination:\u003c\/strong\u003e If resin is accidentally exposed to metal-containing buffer, regenerate by extensive washing with metal-free buffer, or contact us for guidance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling or modification:\u003c\/strong\u003e Any chemical modification (e.g., with ligands, dyes, or functional groups) should use metal-free or purified reagents to avoid reintroducing metals.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e500 mL of high-density metal-free agarose resin\u003c\/li\u003e\n\u003cli\u003ePurified to minimize trace metal contamination\u003c\/li\u003e\n\u003cli\u003eReady for direct use or further derivatization with metal-free reagents\u003c\/li\u003e\n\u003cli\u003eLarge volume suitable for process-scale and developmental applications\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eEliminates trace metal interference in sensitive assays and binding studies\u003c\/li\u003e\n\u003cli\u003eCompatible with all standard chromatography modes and further functionalization\u003c\/li\u003e\n\u003cli\u003eLarge volume economical for process development and scale-up\u003c\/li\u003e\n\u003cli\u003eSuitable for in vitro translation, cell culture supplements, and chelation studies\u003c\/li\u003e\n\u003cli\u003eReduces background in spectrophotometric and fluorescence assays sensitive to metal catalysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat specific metal concentrations does this resin meet?\u003c\/strong\u003e\u003cbr\u003eThis resin is manufactured to minimize trace metals. Request the COA\/TDS for ICP-MS analysis or specific metal concentration limits for your lot.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I couple proteins or ligands to this resin?\u003c\/strong\u003e\u003cbr\u003eYes, using standard agarose derivatization methods (glyoxal, glutaraldehyde, tosyl, CNBr activation). Use metal-free or purified reagents and buffers to maintain metal-free status.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I know if my application is sensitive to the trace metal background?\u003c\/strong\u003e\u003cbr\u003eIf your assay or binding study includes metal chelators (EDTA, EGTA, orthophenanthroline), measures a metal-dependent enzyme, or uses colorimetric\/fluorescence endpoints, metal background could be significant. Run a control with standard resin to assess metal interference.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between this and standard high-density resin?\u003c\/strong\u003e\u003cbr\u003eStandard resins may acquire trace metals during manufacturing or handling. This product is processed and stored specifically to minimize metal pickup, making it suitable for trace-metal-sensitive applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIs this resin suitable for IMAC (immobilized metal affinity chromatography)?\u003c\/strong\u003e\u003cbr\u003eIMAC requires immobilized metal ions, which you add during derivatization. This metal-free resin is ideal as the starting matrix when you want to control metal ion loading precisely and avoid background metal interference.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use standard (non-metal-free) buffers with this resin?\u003c\/strong\u003e\u003cbr\u003eIf your application is metal-sensitive, no. Use buffers prepared with metal-free or extensively purified water and prepared in plastic, not glassware. Pre-treat buffers through this resin if needed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eUeda EK, Gout PW, Morganti L. Current and prospective applications of metal ion-protein binding. \u003cem\u003eJ Chromatogr A.\u003c\/em\u003e 2003;988(1):1-23.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0021-9673(02)02057-5\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBilkova Z, Shabani E, Moravek O, Korecka L, Slovakova M, Jankovicova B. Fundamentals of protein affinity chromatography: How to prepare and properly use an affinity matrix. \u003cem\u003eJ Chromatogr A.\u003c\/em\u003e 2025;1756:466061.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.chroma.2025.466061\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSubramanian A. Immunoaffinity chromatography. \u003cem\u003eMol Biotechnol.\u003c\/em\u003e 2002;20(1):41-7.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1385\/MB:20:1:041\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eFitzgerald J, Leonard P, Darcy E, O'Kennedy R. Immunoaffinity chromatography. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2011;681:35-59.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-60761-913-0_3\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813556416810,"sku":"BTS-B2025392","price":1175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025392.png?v=1790897771","url":"https:\/\/bluetigerscientific.com\/products\/high-density-metal-free-resin","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}