{"product_id":"high-density-glyoxal-6bcl","title":"High Density Glyoxal 6BCL","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHigh Density Glyoxal 6BCL – Catalog #B2025408\u003c\/h2\u003e\n\u003cp\u003eHigh Density Glyoxal 6BCL (Catalog #B2025408) is a pre-activated affinity chromatography resin based on 6% crosslinked agarose with high-density glyoxal groups. The glyoxal (ethanedial) aldehyde groups react directly with primary amino groups on proteins and ligands to form stable Schiff base conjugates without additional activation. The higher 6% crosslinking compared to 4% variants provides greater mechanical rigidity, enabling higher column flow rates and improved performance under load in large-scale purification and process-scale applications. Supplied as 25 mL of resin, it is ready for immediate use in enzyme immobilization, antibody purification, and affinity chromatography workflows.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025408\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAgarose crosslinking:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e6%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eReactive groups:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlyoxal (aldehyde)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCoupling chemistry:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLysine primary amine → Schiff base (imine) conjugate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eEnzyme immobilization, large-scale antibody purification, process-scale affinity chromatography, bioaffinity separations, high-throughput protein purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlyoxal resin, glyoxyl agarose, aldehyde agarose, high-density coupling, 6% crosslinked, pre-activated chromatography resin, affinity matrix, enzyme coupling support\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eGlyoxal groups provide a direct, efficient pathway for covalent protein immobilization via primary amines (lysines, N-terminal amines) without requiring intermediate activation or linker chemistry. The reaction forms Schiff base (imine) conjugates that are highly stable under physiological and slightly acidic conditions. High-density glyoxal groups enable multipoint attachment, which enhances enzyme stability and can preserve or improve catalytic performance by constraining conformational motion.\u003c\/p\u003e\n\u003cp\u003eThe 6% crosslinking in this variant offers several advantages over 4% crosslinked analogs: higher bead rigidity permits higher packing densities, better resistance to column compression, and higher flow rates under load—important for process-scale and large-volume applications. The trade-off is slightly lower apparent porosity and potentially slower diffusion of very large proteins, making 6% crosslinked material most suitable for biomolecules up to ~100 kDa.\u003c\/p\u003e\n\u003cp\u003eAgarose is a natural, hydrophilic polysaccharide matrix that is inert to most proteins and provides minimal non-specific binding. Its open-pore structure and high surface area support high binding capacities.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eLarge-scale or process-scale affinity chromatography\u003c\/li\u003e\n\u003cli\u003eEnzyme immobilization for biocatalysis and cofactor-dependent reactions\u003c\/li\u003e\n\u003cli\u003ePurification of antibodies and binding proteins from cell culture supernatant\u003c\/li\u003e\n\u003cli\u003eCapture and enrichment of biomarkers from plasma or other complex samples\u003c\/li\u003e\n\u003cli\u003eRepeated-use affinity columns for routine purification\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eCouple ligands in appropriate buffer (0.1 M bicarbonate pH 9–10 or 0.1 M phosphate pH 7–8) at 4°C or room temperature for 4–24 hours. Allow adequate contact time for multipoint attachment. After coupling, block remaining aldehyde groups with ethanolamine or glycine to reduce non-specific binding. Pack in a column under gravity or low pressure to avoid compression and maintain even flow.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling buffer:\u003c\/strong\u003e Choose pH 9–10 (bicarbonate) for most proteins; pH 7–8 (phosphate) may be used if solubility is limited at higher pH.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLigand concentration:\u003c\/strong\u003e Typical coupling uses 10–100 µg\/mL of ligand protein in coupling buffer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking:\u003c\/strong\u003e After coupling, treat with 1 M ethanolamine (pH 9) or 1 M glycine (pH 8) for 1–2 hours to inactivate unreacted aldehyde groups and reduce non-specific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColumn packing:\u003c\/strong\u003e Pack under gravity or low pressure; high packing density risks uneven flow and bead compaction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage of conjugate:\u003c\/strong\u003e Store the packed or loose coupled resin at 2–8°C in storage buffer (PBS or phosphate, pH 7.4) with 20% ethanol or 0.05% sodium azide to prevent microbial growth.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow rate:\u003c\/strong\u003e The 6% crosslinking supports higher flow rates than 4% at equivalent pressure; optimize for your column size and ligand.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 mL of pre-activated High Density Glyoxal 6BCL agarose resin\u003c\/li\u003e\n\u003cli\u003eReady-to-use, no additional activation required\u003c\/li\u003e\n\u003cli\u003e6% crosslinking for improved mechanical rigidity and flow capacity\u003c\/li\u003e\n\u003cli\u003eHigh-density glyoxal groups for efficient, multipoint protein coupling\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eDirect one-step coupling via primary amines without additional reagents or intermediates\u003c\/li\u003e\n\u003cli\u003eStable Schiff base linkages resistant to dissociation under typical use\u003c\/li\u003e\n\u003cli\u003eMultipoint attachment enhances enzyme stability and activity retention\u003c\/li\u003e\n\u003cli\u003e6% crosslinking provides mechanical rigidity for large-scale and high-flow applications\u003c\/li\u003e\n\u003cli\u003eHigh-density glyoxal groups support large biomolecule loads\u003c\/li\u003e\n\u003cli\u003eInert agarose matrix minimizes non-specific binding\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the coupling capacity?\u003c\/strong\u003e\u003cbr\u003eCapacity depends on the ligand, its lysine content, and coupling conditions. Request the COA\/TDS for capacity data with reference proteins, or test your ligand of interest.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShould I choose 4% or 6% crosslinked glyoxal agarose?\u003c\/strong\u003e\u003cbr\u003e4% provides higher porosity, better for large proteins (\u0026gt;100 kDa); 6% provides higher rigidity and better flow, ideal for process-scale and smaller ligands. Choose based on your application and ligand size.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the expected coupling efficiency with my protein?\u003c\/strong\u003e\u003cbr\u003eEfficiency depends on accessible lysine residues, pH, and reaction time. Request reference data for similar proteins, or run a small-scale test with your ligand.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I re-use this resin?\u003c\/strong\u003e\u003cbr\u003eGlyoxal-coupled resins can be partially regenerated, but residual ligand and repeated cycles reduce reusability. Contact us for regeneration protocols specific to your ligand.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDo I need to block unreacted aldehyde groups?\u003c\/strong\u003e\u003cbr\u003eBlocking with ethanolamine or glycine is strongly recommended to minimize non-specific binding and improve selectivity in affinity chromatography.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the relationship between crosslinking and binding capacity?\u003c\/strong\u003e\u003cbr\u003eHigher crosslinking increases mechanical strength but slightly reduces surface area. 6% is optimized for large-scale applications; 4% offers higher capacity per unit volume for analytical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLópez-Gallego F, Montes T, Fuentes M, Alonso N, Grazu V, Betancor L, Guisán JM, Fernández-Lafuente R. Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports. \u003cem\u003eJ Biotechnol.\u003c\/em\u003e 2005;116(1):1-10.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jbiotec.2004.09.015\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLópez-Gallego F, Fernandez-Lorente G, Rocha-Martín J, Bolivar JM, Mateo C, Guisan JM. Multi-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial Enzymes. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2020;2100:93-107.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-0215-7_5\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eZang B, Ren J, Xu L, Jia L. Direct site-specific immobilization of protein A via aldehyde-hydrazide conjugation. \u003cem\u003eJ Chromatogr B Analyt Technol Biomed Life Sci.\u003c\/em\u003e 2016;1008:132-138.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jchromb.2015.11.019\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSubramanian A. Immunoaffinity chromatography. \u003cem\u003eMol Biotechnol.\u003c\/em\u003e 2002;20(1):41-7.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1385\/MB:20:1:041\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813556547882,"sku":"BTS-B2025408","price":1195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025408.png?v=1790897777","url":"https:\/\/bluetigerscientific.com\/products\/high-density-glyoxal-6bcl","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}