{"product_id":"high-density-glyoxal-4bcl","title":"High Density Glyoxal 4BCL","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHigh Density Glyoxal 4BCL – Catalog #B2025390\u003c\/h2\u003e\n\u003cp\u003eHigh Density Glyoxal 4BCL (Catalog #B2025390) is a pre-activated affinity chromatography resin based on 4% crosslinked agarose with a high density of glyoxal groups. The glyoxal (ethanedial) aldehyde groups react directly with primary amino groups (lysines, N-terminal amines) on proteins or ligands to form stable Schiff base conjugates without additional activation steps. Supplied as 25 mL of resin ready to use, it simplifies the immobilization of enzymes, antibodies, and binding proteins for affinity purification and enzyme-catalyzed applications.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025390\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAgarose crosslinking:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e4%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eReactive groups:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlyoxal (aldehyde)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCoupling chemistry:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLysine primary amine → Schiff base (imine) conjugate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eEnzyme immobilization, antibody affinity chromatography, protein-ligand coupling, bioaffinity separations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlyoxal resin, glyoxyl agarose, aldehyde agarose, high-density coupling, pre-activated chromatography resin, affinity matrix, enzyme coupling support\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eGlyoxal groups on agarose matrices provide a direct, efficient route to covalent immobilization of proteins via primary amines without the intermediate steps required by some other coupling chemistries. The reaction forms Schiff base linkages that are highly stable and resist elution under typical chromatographic conditions. The high density of glyoxal groups on this resin ensures multipoint attachment, which enhances stability and can improve the catalytic performance of immobilized enzymes by reducing conformational flexibility.\u003c\/p\u003e\n\u003cp\u003eAgarose is a natural polysaccharide from red algae with a porous, hydrophilic matrix that is inert to most biomolecules, permitting high flow rates and minimal nonspecific binding. The 4% crosslinking provides a balance between porosity, mechanical stability, and diffusion rate suitable for many protein applications.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eImmobilization of enzymes for biocatalysis and cofactor-dependent reactions\u003c\/li\u003e\n\u003cli\u003eAffinity chromatography for antibody or ligand purification\u003c\/li\u003e\n\u003cli\u003eConstruction of immunosorbent columns for bioaffinity separation\u003c\/li\u003e\n\u003cli\u003eHigh-throughput protein purification and biomarker detection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eCouple ligands immediately after equilibration in coupling buffer (typically 0.1 M sodium bicarbonate, pH 9–10, or 0.1 M sodium phosphate, pH 7–8, depending on the ligand). Allow adequate contact time (typically 4–24 hours at 4°C or room temperature) to ensure multipoint attachment. After coupling, block remaining aldehyde groups with ethanolamine, glycine, or other amine to reduce non-specific binding and improve selectivity.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling buffer:\u003c\/strong\u003e Use pH 7–10 buffers depending on ligand isoelectric point and desired coupling kinetics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking:\u003c\/strong\u003e After coupling, treat with 1 M ethanolamine (pH 9) or 1 M glycine (pH 8) for 1–2 hours to neutralize unreacted aldehyde groups.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eColumn packing:\u003c\/strong\u003e Pack under gravity or low pressure to avoid bead compaction and maintain flow characteristics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage of conjugate:\u003c\/strong\u003e Store at 2–8°C in storage buffer (typically PBS or 0.1 M phosphate, pH 7.4) with 20% ethanol to inhibit microbial growth.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 mL of pre-activated High Density Glyoxal 4BCL agarose resin\u003c\/li\u003e\n\u003cli\u003eReady-to-use, no additional activation required\u003c\/li\u003e\n\u003cli\u003eHigh glyoxal density for efficient, multipoint protein coupling\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eDirect, one-step coupling via primary amines without additional reagents\u003c\/li\u003e\n\u003cli\u003eStable Schiff base linkages resistant to elution under normal use\u003c\/li\u003e\n\u003cli\u003eMultipoint attachment enhances enzyme stability and activity retention\u003c\/li\u003e\n\u003cli\u003eHigh-density glyoxal groups support large biomolecule loads\u003c\/li\u003e\n\u003cli\u003eInert agarose matrix minimizes non-specific binding\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the expected coupling efficiency?\u003c\/strong\u003e\u003cbr\u003eCoupling efficiency depends on the ligand's lysine content, pH, and contact time. Request the COA\/TDS for empirical coupling data with reference proteins on this lot, or test with your protein of interest.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I re-use this resin after stripping the coupled ligand?\u003c\/strong\u003e\u003cbr\u003eGlyoxal resins can be partially regenerated, but residual ligand and repeated cycles may reduce reusability. Contact us for specific protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between 4% and 6% crosslinked agarose?\u003c\/strong\u003e\u003cbr\u003e4% crosslinking provides lower density and higher porosity, suitable for larger proteins; 6% provides higher stiffness and better flow under load. Choose based on your ligand and application.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDo I need to block unreacted groups?\u003c\/strong\u003e\u003cbr\u003eBlocking with ethanolamine or glycine is recommended to reduce non-specific binding and improve selectivity in affinity chromatography.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat buffers work best for coupling?\u003c\/strong\u003e\u003cbr\u003eCoupling is typically most efficient at pH 9–10 (bicarbonate buffer); however, pH 7–8 buffers (phosphate) can also be used if the ligand has limited solubility at higher pH.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I couple antibodies and enzymes to the same batch of resin?\u003c\/strong\u003e\u003cbr\u003eYes, if both have accessible primary amines. Optimal coupling conditions may differ; run small-scale tests to optimize for your ligands.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLópez-Gallego F, Montes T, Fuentes M, Alonso N, Grazu V, Betancor L, Guisán JM, Fernández-Lafuente R. Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports. \u003cem\u003eJ Biotechnol.\u003c\/em\u003e 2005;116(1):1-10.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jbiotec.2004.09.015\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLópez-Gallego F, Fernandez-Lorente G, Rocha-Martín J, Bolivar JM, Mateo C, Guisan JM. Multi-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial Enzymes. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2020;2100:93-107.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-0215-7_5\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eZang B, Ren J, Xu L, Jia L. Direct site-specific immobilization of protein A via aldehyde-hydrazide conjugation. \u003cem\u003eJ Chromatogr B Analyt Technol Biomed Life Sci.\u003c\/em\u003e 2016;1008:132-138.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jchromb.2015.11.019\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSubramanian A. Immunoaffinity chromatography. \u003cem\u003eMol Biotechnol.\u003c\/em\u003e 2002;20(1):41-7.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1385\/MB:20:1:041\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813554581802,"sku":"BTS-B2025390","price":1175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025390.png?v=1790897668","url":"https:\/\/bluetigerscientific.com\/products\/high-density-glyoxal-4bcl","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}