{"product_id":"high-density-glyoxal-4bcl-magnetic","title":"High Density Glyoxal 4BCL Magnetic","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHigh Density Glyoxal 4BCL Magnetic – Catalog #B2025445\u003c\/h2\u003e\n\u003cp\u003eHigh Density Glyoxal 4BCL Magnetic (Catalog #B2025445) is a pre-activated magnetic affinity resin that combines glyoxal-reactive agarose with magnetic iron oxide particles for rapid, hands-free sample processing. The glyoxal groups form direct Schiff base conjugates with primary amines on proteins and ligands without additional activation, while the magnetic core enables quick separation by external magnet. Supplied as 2 mL of ready-to-use magnetic resin, it streamlines enzyme immobilization, immunoprecipitation, and bioaffinity purification workflows that previously required centrifugation or column packing.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025445\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAgarose crosslinking:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e6%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eReactive groups:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlyoxal (aldehyde), magnetic iron oxide core\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCoupling chemistry:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLysine primary amine → Schiff base (imine) conjugate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSeparation method:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic separation (no centrifugation required)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eRapid immunoprecipitation, enzyme immobilization, biomarker capture, high-throughput affinity purification, magnetic bead assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic glyoxal resin, magnetic agarose, glyoxyl magnetic beads, pre-activated magnetic support, aldehyde magnetic particles, immunoprecipitation beads, rapid affinity separation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eMagnetic glyoxal agarose combines the chemical efficiency of glyoxal coupling with the operational simplicity of magnetic separation. Glyoxal groups on agarose provide direct access to lysine primary amines, forming stable Schiff base conjugates that resist dissociation under typical chromatographic and washing conditions. The 6% crosslinked agarose provides good mechanical rigidity while maintaining sufficient porosity for protein diffusion. Iron oxide cores allow rapid, hands-free separation with a permanent magnet, eliminating the need for centrifugation, filtration, or column packing—ideal for batch immunoprecipitation, rapid antibody capture, and high-throughput screening.\u003c\/p\u003e\n\u003cp\u003eThe magnetic bead format is particularly advantageous in automated and high-throughput workflows where sample volume is small (microliters to milliliters) and rapid turnaround is essential. Multipoint attachment via the high-density glyoxal groups enhances the stability of immobilized proteins compared to single-point attachment, often preserving or improving catalytic activity.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eRapid immunoprecipitation and immunoaffinity purification\u003c\/li\u003e\n\u003cli\u003eImmobilization of capture antibodies for immunoassays\u003c\/li\u003e\n\u003cli\u003eEnzymatic coupling for cofactor-dependent biocatalysis\u003c\/li\u003e\n\u003cli\u003eBiomarker isolation and enrichment from complex samples\u003c\/li\u003e\n\u003cli\u003eMultiplexed bead-based assays and droplet microfluidics\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eResuspend the magnetic resin thoroughly before use (a fine suspension may settle upon storage). Couple ligands in 0.1–0.5 mL of appropriate buffer (pH 7–10, typically 0.1 M bicarbonate or phosphate) at room temperature or 4°C for 4–24 hours. After coupling, capture with a magnet, remove the supernatant, and block unreacted aldehyde groups with ethanolamine or glycine. Wash thoroughly with storage buffer before use.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnet choice:\u003c\/strong\u003e Use a magnetic separator appropriate for the sample volume (96-well plate magnet, tube rack magnet, or handheld magnet).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling efficiency:\u003c\/strong\u003e Multipoint attachment is efficient; coupling times of 4–24 hours at room temperature or 4°C are typical.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking:\u003c\/strong\u003e Treat with 1 M ethanolamine (pH 9) or 1 M glycine (pH 8) for 1–2 hours to reduce non-specific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBead recovery:\u003c\/strong\u003e Apply magnet for 1–3 minutes to fully sediment beads; carefully remove supernatant with pipette without disrupting the pellet.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBatch variability:\u003c\/strong\u003e Magnetic bead suspensions may vary in apparent sedimentation rate; resuspend and vortex gently before each use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e2 mL of pre-activated High Density Glyoxal 4BCL magnetic agarose resin\u003c\/li\u003e\n\u003cli\u003eReady-to-use magnetic support requiring no additional activation\u003c\/li\u003e\n\u003cli\u003eHands-free magnetic separation without centrifugation or filtration\u003c\/li\u003e\n\u003cli\u003eHigh-density glyoxal groups for efficient, multipoint protein coupling\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eDirect, one-step glyoxal coupling of primary amines without intermediates\u003c\/li\u003e\n\u003cli\u003eRapid magnetic separation eliminates centrifugation and column packing\u003c\/li\u003e\n\u003cli\u003eMultipoint attachment stabilizes immobilized enzymes and antibodies\u003c\/li\u003e\n\u003cli\u003eIdeal for high-throughput and automated sample processing\u003c\/li\u003e\n\u003cli\u003eCompatible with standard magnetic separators and microfluidic devices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eDo I need a special magnet?\u003c\/strong\u003e\u003cbr\u003eAny permanent magnet strong enough to sediment iron oxide particles will work. Microplate magnetizers, tube rack magnets, and handheld magnets are all compatible; choose based on your sample format and workflow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between 4% and 6% crosslinked magnetic agarose?\u003c\/strong\u003e\u003cbr\u003e6% crosslinking (used in this product) provides higher bead rigidity and faster magnetic sedimentation; 4% offers higher porosity suitable for very large proteins. Choose based on your ligand size and desired sedimentation speed.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I re-use magnetic beads after stripping the coupled ligand?\u003c\/strong\u003e\u003cbr\u003eMagnetic beads can be partially regenerated, but residual ligand and repeated cycles reduce reusability. Contact us for protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I measure coupling efficiency?\u003c\/strong\u003e\u003cbr\u003eRequest the COA\/TDS for reference coupling data with standard proteins, or test your ligand at small scale. Efficiency depends on protein lysine content and buffer pH.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhy are some beads still suspended after applying the magnet?\u003c\/strong\u003e\u003cbr\u003eThis is normal with magnetic bead suspensions. Apply the magnet longer (2–3 minutes) or use a stronger magnet. Gently vortex the tube first to ensure a uniform suspension.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use this in plate readers or fluorescence assays?\u003c\/strong\u003e\u003cbr\u003eYes. After magnetic capture, you can wash and then assay the immobilized protein directly. Magnetic beads may affect fluorescence readings; run controls to assess signal loss.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLópez-Gallego F, Montes T, Fuentes M, Alonso N, Grazu V, Betancor L, Guisán JM, Fernández-Lafuente R. Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports. \u003cem\u003eJ Biotechnol.\u003c\/em\u003e 2005;116(1):1-10.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jbiotec.2004.09.015\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLópez-Gallego F, Fernandez-Lorente G, Rocha-Martín J, Bolivar JM, Mateo C, Guisan JM. Multi-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial Enzymes. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2020;2100:93-107.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-0215-7_5\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSubramanian A. Immunoaffinity chromatography. \u003cem\u003eMol Biotechnol.\u003c\/em\u003e 2002;20(1):41-7.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1385\/MB:20:1:041\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBilkova Z, Shabani E, Moravek O, Korecka L, Slovakova M, Jankovicova B. Fundamentals of protein affinity chromatography: How to prepare and properly use an affinity matrix. \u003cem\u003eJ Chromatogr A.\u003c\/em\u003e 2025;1756:466061.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.chroma.2025.466061\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813555073322,"sku":"BTS-B2025445","price":1245.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025445.png?v=1790897698","url":"https:\/\/bluetigerscientific.com\/products\/high-density-glyoxal-4bcl-magnetic","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}