{"product_id":"hexa-his","title":"Hexa-His","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHexa-His (His6-Tag) – Catalog #B2026696\u003c\/h2\u003e\n\u003cp\u003eHexa-His (Catalog #B2026696) is a peptide sequence of six consecutive histidine residues (His–His–His–His–His–His) used extensively in protein engineering as a purification tag. Supplied as 1 mg lyophilized powder, this peptide is incorporated into recombinant proteins during expression to enable rapid, single-step purification via immobilized metal affinity chromatography (IMAC). Histidine residues coordinate strongly with divalent metal ions (nickel, cobalt, zinc), allowing tagged proteins to be selectively captured on metal-loaded resins and eluted under mild, non-denaturing conditions. The His6-tag is one of the most widely used affinity tags due to its small size (approximately 0.8 kDa), minimal impact on protein function, and ease of removal if needed.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2026696\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLyophilized Powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSequence:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHis–His–His–His–His–His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eTypical molecular weight:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e~850 Da\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eIMAC purification of His-tagged recombinant proteins, identification and isolation of His-tagged fusion proteins, affinity detection assays, chromatin immunoprecipitation (ChIP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHis-tag, His6-tag, Hexa-histidine, Hexahistidine, IMAC, immobilized metal affinity chromatography, His-tagged protein, protein purification tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eThe His6-tag is based on the strong coordination chemistry between histidine imidazole groups and divalent metal ions, particularly nickel (II) and cobalt (II). When a His-tagged protein is loaded onto a metal-loaded chromatography resin (typically Ni-NTA or Ni-IDA), the histidine residues bind to the immobilized metal, while non-tagged proteins flow through. Bound protein is eluted by either increasing imidazole concentration (competitive elution) or decreasing pH to protonate histidine, weakening the metal coordination.\u003c\/p\u003e\n\u003cp\u003eThe His6-tag offers several advantages: it is small (0.8 kDa, minimal impact on protein structure and function), widely recognized by antibodies and detection systems, and can be incorporated at either the N- or C-terminus of a protein. Many proteases (e.g., TEV, thrombin) can cleave the tag post-purification if a tag-free protein is desired. IMAC is generally faster and more specific than other affinity tags, making it the standard choice for high-throughput recombinant protein production.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eSingle-step, high-capacity purification of recombinant proteins from crude cell lysates\u003c\/li\u003e\n\u003cli\u003eRapid identification and isolation of transiently expressed or weakly expressed proteins\u003c\/li\u003e\n\u003cli\u003eProtein pull-down and co-immunoprecipitation assays\u003c\/li\u003e\n\u003cli\u003eIntegrated purification and detection workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore lyophilized peptide at –20°C. Before use, dissolve in appropriate buffer (e.g., Tris or phosphate buffer, pH 7–8) to the desired concentration. The His-tag peptide itself is not typically used directly as a purification substrate; instead, it serves as a reference or is incorporated into recombinant proteins via genetic engineering.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncorporation into recombinant proteins:\u003c\/strong\u003e The His6 sequence is typically added to expression plasmids via molecular cloning, allowing the target protein to be expressed as a His-fusion during recombinant protein production.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification of His-tagged proteins:\u003c\/strong\u003e Use Ni-NTA, Ni-IDA, Cobalt-IDA, or other metal-loaded resins designed for IMAC.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding:\u003c\/strong\u003e Apply clarified cell lysate to a pre-equilibrated IMAC column; His-tagged protein will bind while non-tagged protein flows through.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWash:\u003c\/strong\u003e Wash with binding buffer containing a small amount of imidazole (typically 5–20 mM) to reduce non-specific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Elute with higher imidazole concentration (typically 250–500 mM) or lower pH (pH 4.5–5.0).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 mg of Hexa-His (His6) peptide, lyophilized\u003c\/li\u003e\n\u003cli\u003eA reference standard for His-tag detection and identification\u003c\/li\u003e\n\u003cli\u003eSmall, well-characterized peptide tag for recombinant protein engineering\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eSmall size (0.8 kDa) minimizes impact on protein structure and function\u003c\/li\u003e\n\u003cli\u003eRapid, single-step purification on inexpensive IMAC resins\u003c\/li\u003e\n\u003cli\u003eMild elution conditions maintain protein activity and stability\u003c\/li\u003e\n\u003cli\u003eWide availability of detection antibodies and compatible reagents\u003c\/li\u003e\n\u003cli\u003eCan be fused at N- or C-terminus or removed post-purification via protease cleavage\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between His-tag and His6-tag?\u003c\/strong\u003e\u003cbr\u003eHis6-tag (Hexa-His) specifically refers to six histidine residues in tandem. Longer tags (His8, His10) are also used but are less common. Six histidines provide an optimal balance of binding strength and minimal size impact.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I remove the His-tag after purification?\u003c\/strong\u003e\u003cbr\u003eYes. Many proteases (TEV, thrombin, factor Xa, enterokinase) recognize specific cleavage sites engineered between the tag and the target protein, allowing tag removal post-purification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDoes the His-tag affect protein function?\u003c\/strong\u003e\u003cbr\u003eUsually not. The His6-tag is small and flexible, but some proteins may be affected, particularly if the tag is positioned near the active site. Testing is recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat resin should I use for His-tag purification?\u003c\/strong\u003e\u003cbr\u003eNickel-NTA or Nickel-IDA resins are the most common; Cobalt-IDA is an alternative with different selectivity and elution characteristics. Choose based on your protein and desired purity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the typical yield?\u003c\/strong\u003e\u003cbr\u003eThis depends on your expression system, protein stability, and purification conditions. Many users achieve 50–95% recovery of soluble His-tagged protein from clarified lysate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a COA or TDS?\u003c\/strong\u003e\u003cbr\u003eYes. Request a quote or contact us and we will provide available lot documentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSpriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. \u003cem\u003eMethods Enzymol.\u003c\/em\u003e 2015;559:1-15.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/bs.mie.2014.11.003\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eTerpe K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. \u003cem\u003eAppl Microbiol Biotechnol.\u003c\/em\u003e 2003;60(5):523-33.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/s00253-002-1158-6\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLoughran ST, Bree RT, Walls D. Poly-Histidine-Tagged Protein Purification Using Immobilized Metal Affinity Chromatography (IMAC). \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2023;2699:193-223.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-3362-5_11\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813556449578,"sku":"BTS-B2026696","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2026696.png?v=1790897772","url":"https:\/\/bluetigerscientific.com\/products\/hexa-his","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}