{"product_id":"heparin-magnetic-agarose-beads-1","title":"Heparin Magnetic Agarose Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eHeparin Magnetic Agarose Beads – Catalog #B2025508\u003c\/h2\u003e\n\u003cp\u003eHeparin Magnetic Agarose Beads (Catalog #B2025508) are magnetic agarose particles functionalized with heparin, a highly sulfated glycosaminoglycan that binds a broad spectrum of proteins. Supplied as a 1 mL solution, these beads facilitate rapid, high-capacity purification of heparin-binding proteins including growth factors, cytokines, chemokines, extracellular matrix proteins, and other heparin-interactive biomolecules. The magnetic core enables fast separation using standard magnetic particle handlers or external magnets, making this resin particularly suitable for high-throughput applications and automated workflows.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025508\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCoating:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHeparin (glycosaminoglycan)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity purification of growth factors and cytokines, heparin binding protein isolation, extracellular vesicle fractionation, protein-protein interaction studies, sample enrichment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHeparin magnetic beads, heparin-coated beads, heparin affinity beads, growth factor purification, cytokine isolation, magnetic protein purification, heparin-agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eHeparin is a linear, highly sulfated glycosaminoglycan composed of repeating disaccharide units (glucuronic acid and glucosamine) with extensive negative charges. This structure allows heparin to interact with a diverse array of proteins through electrostatic and specific binding interactions. Well-known heparin-binding proteins include acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and numerous chemokines and extracellular matrix proteins.\u003c\/p\u003e\n\u003cp\u003eHeparin-coated affinity resins have been used for decades to purify these proteins; the addition of magnetic properties dramatically improves handling and throughput. Heparin magnetic agarose beads combine the proven specificity of heparin-based chromatography with the convenience of magnetic separation, making them ideal for both analytical and preparative protein purification.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eRapid purification of growth factors and cytokines\u003c\/li\u003e\n\u003cli\u003eIsolation of heparin-binding extracellular proteins\u003c\/li\u003e\n\u003cli\u003eSample enrichment and fractionation from complex mixtures\u003c\/li\u003e\n\u003cli\u003eMagnetic bead-based separation for high-throughput and automated workflows\u003c\/li\u003e\n\u003cli\u003ePull-down assays for heparin-binding protein interactions\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Before each experiment, mix gently to resuspend beads uniformly. Avoid excessive agitation, which may cause aggregation.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample preparation:\u003c\/strong\u003e Clarify samples (e.g., cell lysates, culture supernatants) by centrifugation or filtration before loading to minimize bead clogging.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding:\u003c\/strong\u003e Incubate your heparin-binding protein sample with beads in bind\/wash buffer at 4°C or room temperature (typically 1–4 hours) with gentle mixing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeparation:\u003c\/strong\u003e Place the tube in a magnetic stand; beads will collect at the tube wall in 1–2 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Separate magnetically and wash 3–5 times with bind\/wash buffer to remove unbound protein and salts.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Elute bound protein with increased salt (e.g., 0.5–1.0 M NaCl) or other appropriate elution buffer, or resuspend beads directly in SDS-PAGE sample buffer for analysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 mL of heparin-coated magnetic agarose particles in solution\u003c\/li\u003e\n\u003cli\u003eReady-to-use for rapid protein purification without centrifugation\u003c\/li\u003e\n\u003cli\u003eHigh binding capacity for heparin-binding proteins\u003c\/li\u003e\n\u003cli\u003eMagnetic properties for easy separation and high-throughput applications\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eBroad specificity for a diverse range of heparin-binding proteins\u003c\/li\u003e\n\u003cli\u003eRapid purification without centrifugation or gravity flow\u003c\/li\u003e\n\u003cli\u003eProven decades of use in heparin-based affinity chromatography\u003c\/li\u003e\n\u003cli\u003eCompatible with batch, column, and automated separation methods\u003c\/li\u003e\n\u003cli\u003eIdeal for isolating growth factors, cytokines, and extracellular proteins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat proteins bind heparin?\u003c\/strong\u003e\u003cbr\u003eHeparin binds growth factors (FGF, VEGF, HGF), cytokines, chemokines, extracellular matrix proteins, and many other heparin-interactive proteins through electrostatic and specific interactions. The specificity is broader than His-tag or GST-tag systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat salt concentration should I use for elution?\u003c\/strong\u003e\u003cbr\u003eTypical elution uses 0.5–1.0 M NaCl in your binding buffer. Start with 0.5 M and increase if needed. Specific conditions depend on your protein and should be optimized.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads in a column?\u003c\/strong\u003e\u003cbr\u003eYes. Although the magnetic property is designed for magnetic separation, you can also pack this resin in a gravity flow or low-pressure column.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I prevent non-specific binding?\u003c\/strong\u003e\u003cbr\u003eOptimize pH and buffer conditions; include carrier proteins (e.g., BSA) if needed; and wash thoroughly with bind\/wash buffer before elution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre the beads suitable for automation?\u003c\/strong\u003e\u003cbr\u003eYes. Magnetic bead-based purification is well-suited to robotic liquid handlers and high-throughput platforms.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a COA or TDS?\u003c\/strong\u003e\u003cbr\u003eYes. Request a quote or contact us and we will provide available lot documentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBarnes B, Caws T, Thomas S, Shephard AP, Corteling R, Hole P, Bracewell DG. Investigating heparin affinity chromatography for extracellular vesicle purification and fractionation. \u003cem\u003eJ Chromatogr A.\u003c\/em\u003e 2022;1670:462987.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.chroma.2022.462987\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBolten SN, Rinas U, Scheper T. Heparin: role in protein purification and substitution with animal-component free material. \u003cem\u003eAppl Microbiol Biotechnol.\u003c\/em\u003e 2018;102(20):8647-8660.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/s00253-018-9263-3\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eMorris J, Jayanthi S, Langston R, Daily A, Kight A, McNabb DS, Henry R, Kumar TKS. Heparin-binding peptide as a novel affinity tag for purification of recombinant proteins. \u003cem\u003eProtein Expr Purif.\u003c\/em\u003e 2016;126:93-103.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.pep.2016.05.013\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813555728682,"sku":"BTS-B2025508","price":1245.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025508_feadd17a-552a-4cf6-86e4-0b42d0debf79.png?v=1790897729","url":"https:\/\/bluetigerscientific.com\/products\/heparin-magnetic-agarose-beads-1","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}