{"product_id":"green-carboxyl-fluorescent-nanoparticle-167-500-nm","title":"Green Carboxyl Fluorescent Nanoparticle (167–500 nm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eGreen Carboxyl Fluorescent Nanoparticle (167–500 nm) – Catalog #B2026047\u003c\/h2\u003e\n\u003cp\u003eGreen Carboxyl Fluorescent Nanoparticles (Catalog #B2026047) are colloidal suspensions of nanometer-scale particles with bright green fluorescence and reactive carboxyl (–COOH) surface groups. Supplied as 10 mL, these nanoparticles combine ease of conjugation with high quantum yield and excellent photostability, making them suitable for cell labeling, tracking, imaging, and detection workflows. The carboxyl groups enable rapid, stable attachment of proteins, antibodies, peptides, and other targeting molecules via standard carbodiimide (EDC\/NHS) or amine coupling chemistries.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2026047\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePellets\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSize range:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e167–500 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eFluorescence (excitation\/emission):\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGreen (~500\/520 nm typical)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface chemistry:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCarboxyl-functionalized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCell labeling, fluorescence microscopy, flow cytometry, bioconjugation, particle tracking, multiplexing assays, immunofluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGreen fluorescent nanoparticles, carboxyl nanoparticles, fluorescent microspheres, green-emitting particles, cell labeling beads, bioconjugate beads, fluorescent probes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eFluorescent nanoparticles offer significant advantages over traditional dyes and proteins: bright, photostable fluorescence with low photobleaching; small size for easy cellular uptake or co-localization with target molecules; and quantifiable optical properties for detection and sorting. Carboxyl-functionalized nanoparticles are particularly versatile because the reactive carboxyl groups can be activated (via EDC\/NHS or direct carbodiimide coupling) to form amide bonds with amine-containing molecules.\u003c\/p\u003e\n\u003cp\u003eThe 167–500 nm size range is ideal for applications requiring sufficient signal intensity while maintaining nanoscale properties for cell and tissue penetration. Green fluorescence (~500\/520 nm excitation\/emission) is well-separated from autofluorescence and compatible with standard green channel detectors on fluorescence microscopes and flow cytometers.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eDirect cell labeling for flow cytometry and microscopy\u003c\/li\u003e\n\u003cli\u003eConjugation to antibodies for immunofluorescence assays\u003c\/li\u003e\n\u003cli\u003eProtein tagging and affinity tracking\u003c\/li\u003e\n\u003cli\u003eMultiplexed detection in combination with other colors\u003c\/li\u003e\n\u003cli\u003eParticle tracking for cell dynamics and transport studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C and protect from prolonged light exposure. Before use, mix gently to ensure uniform suspension; do not vortex vigorously, as this may cause aggregation.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBioconjugation:\u003c\/strong\u003e Activate carboxyl groups with EDC\/NHS in MES buffer (pH 5–6) for 15–30 minutes, then incubate with your target protein (pH 7–8) for 1–4 hours at room temperature or 4°C overnight.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e After conjugation, centrifuge briefly (if needed) and wash with PBS or storage buffer to remove unreacted particles.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell labeling:\u003c\/strong\u003e Incubate conjugated nanoparticles with cells at 4°C (for surface labeling) or 37°C (for internalization) for 30 minutes to several hours, depending on the target and application.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection:\u003c\/strong\u003e Use standard green filter sets (ex ~488 nm, em ~520 nm) on fluorescence microscopes or flow cytometers.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e10 mL of green-emitting carboxyl-functionalized nanoparticles (167–500 nm)\u003c\/li\u003e\n\u003cli\u003eBright, photostable fluorescence ready for immediate use or bioconjugation\u003c\/li\u003e\n\u003cli\u003eReactive carboxyl groups for protein and antibody coupling\u003c\/li\u003e\n\u003cli\u003eCompatible with standard fluorescence microscopy and flow cytometry instrumentation\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh quantum yield and excellent photostability\u003c\/li\u003e\n\u003cli\u003eCarboxyl surface chemistry enables rapid protein coupling\u003c\/li\u003e\n\u003cli\u003eSuitable for both fixed and live-cell imaging\u003c\/li\u003e\n\u003cli\u003eIdeal for multiplexing when combined with other fluorophore colors\u003c\/li\u003e\n\u003cli\u003eEasier to detect and quantify than small-molecule dyes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the typical fluorescence intensity?\u003c\/strong\u003e\u003cbr\u003eThis depends on the specific particle composition and batch. Intensity is generally higher than small-molecule dyes and lower-quantum-yield quantum dots. Request the COA\/TDS for lot-specific photophysical characterization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use EDC\/NHS coupling?\u003c\/strong\u003e\u003cbr\u003eYes. EDC (N-ethyl-N′-carbodiimide) activates carboxyl groups for amine coupling; NHS increases the reaction efficiency. This is the standard approach for protein conjugation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I prevent particle aggregation?\u003c\/strong\u003e\u003cbr\u003eMix gently, avoid vortexing, and keep particles in suspension. Store at 2–8°C and use sterile, pyrogen-free buffers.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre these particles appropriate for live-cell imaging?\u003c\/strong\u003e\u003cbr\u003eYes, provided they are non-toxic at your working concentration and the conjugate does not interfere with cellular function. Test in your system before large-scale experiments.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a COA or TDS?\u003c\/strong\u003e\u003cbr\u003eYes. Request a quote or contact us and we will provide available lot documentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eQian J, Wang Y, Gao X, Zhan Q, Xu Z, He S. Carboxyl-functionalized and bio-conjugated silica-coated quantum dots as targeting probes for cell imaging. \u003cem\u003eJ Nanosci Nanotechnol.\u003c\/em\u003e 2010;10(3):1668-75.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1166\/jnn.2010.2043\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eJana NR, Erathodiyil N, Jiang J, Ying JY. Cysteine-functionalized polyaspartic acid: a polymer for coating and bioconjugation of nanoparticles and quantum dots. \u003cem\u003eLangmuir.\u003c\/em\u003e 2010;26(9):6503-7.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/la903965t\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHo YP, Leong KW. Quantum dot-based theranostics. \u003cem\u003eNanoscale.\u003c\/em\u003e 2010;2(1):60-8.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1039\/b9nr00178f\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813556023594,"sku":"BTS-B2026047","price":705.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2026047-1.png?v=1790897741","url":"https:\/\/bluetigerscientific.com\/products\/green-carboxyl-fluorescent-nanoparticle-167-500-nm","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}