{"product_id":"glyoxal-4bcl-magnetic-1","title":"Glyoxal 4BCL Magnetic","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eGlyoxal 4BCL Magnetic – Catalog #B2025447\u003c\/h2\u003e\n\u003cp\u003eGlyoxal 4BCL Magnetic (Catalog #B2025447) is a pre-activated magnetic agarose resin designed for rapid, covalent immobilization of ligands via glyoxal-lysine coupling. The resin consists of a 6% crosslinked agarose backbone with reactive glyoxal (1,2-dicarbonaldehyde) groups, combined with magnetic iron oxide particles for easy manipulation using external magnets or magnetic separation equipment. This combination of rapid coupling chemistry and magnetic separation makes it ideal for high-throughput affinity chromatography, enzyme immobilization, and antibody or protein ligand coupling.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025447\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMatrix:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic agarose, 6% crosslinked\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eReactive groups:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlyoxal (aldehyde) groups\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eEnzyme immobilization, antibody coupling, affinity chromatography, protein ligand coupling, one-step affinity purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlyoxal magnetic beads, glyoxyl-agarose, aldehyde coupling, 4% crosslinked agarose, pre-activated resin, magnetic agarose, enzyme immobilization, affinity coupling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eGlyoxal (1,2-dicarbonaldehyde) is a two-carbon dialdehyde that reacts rapidly and specifically with primary amines (lysine, histidine, and free N-terminal amino groups) to form stable Schiff bases, which can be further reduced to secondary amines for permanent coupling. Glyoxal coupling is generally milder than glutaraldehyde, with minimal loss of protein function, and has been extensively used for enzyme immobilization and affinity chromatography.\u003c\/p\u003e\n\u003cp\u003eThe 4BCL designation indicates a 4% crosslinked agarose backbone, providing good flow rates while maintaining mechanical stability. The addition of magnetic particles allows rapid separation of the resin from solution using a magnetic field, dramatically reducing processing time compared to gravity flow or centrifugation.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eOne-step immobilization of enzymes, antibodies, and proteins\u003c\/li\u003e\n\u003cli\u003eAffinity chromatography resins for capture and purification\u003c\/li\u003e\n\u003cli\u003eLigand coupling for screening and binding studies\u003c\/li\u003e\n\u003cli\u003eRapid magnetic separation for high-throughput workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Before use, inspect the resin visually to ensure magnetic particles are uniformly distributed. During coupling reactions, keep the resin in suspension with gentle stirring to maintain uniform contact with the ligand.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling protocol:\u003c\/strong\u003e Incubate your protein ligand with the resin at pH 7.0–7.5 and room temperature for 2–4 hours (or overnight) in appropriate buffer. Exact conditions depend on the protein and should be optimized for your specific ligand.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReduction:\u003c\/strong\u003e For permanent coupling, reduce Schiff bases with sodium borohydride or sodium cyanoborohydride after the coupling reaction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Wash the coupled resin thoroughly with coupling buffer, then with wash buffer, to remove unreacted ligand and chemical reagents.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Place the resin in a magnetic stand; particles will collect at the tube wall in 1–2 minutes, allowing supernatant to be removed by pipetting.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e2 mL of pre-activated magnetic glyoxal-agarose resin\u003c\/li\u003e\n\u003cli\u003e6% crosslinked agarose matrix for mechanical stability\u003c\/li\u003e\n\u003cli\u003eReactive glyoxal groups ready for immediate protein coupling\u003c\/li\u003e\n\u003cli\u003eMagnetic properties for rapid separation and high-throughput workflows\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eOne-step coupling chemistry with minimal protein damage\u003c\/li\u003e\n\u003cli\u003eMagnetic separation eliminates tedious gravity flow or centrifugation\u003c\/li\u003e\n\u003cli\u003eHigh binding capacity and excellent flow characteristics\u003c\/li\u003e\n\u003cli\u003eSuitable for routine batch coupling and high-throughput automation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between glyoxal and glutaraldehyde coupling?\u003c\/strong\u003e\u003cbr\u003eGlyoxal is generally milder and can result in less loss of protein function, though both form stable covalent bonds. Choose based on your protein stability and desired coupling efficiency.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDo I need to reduce the Schiff base after coupling?\u003c\/strong\u003e\u003cbr\u003eFor permanent bonds, yes: use sodium borohydride or sodium cyanoborohydride. Unreduced Schiff bases are reversible and may dissociate under certain conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use this resin in a column format?\u003c\/strong\u003e\u003cbr\u003eYes. Although the magnetic property is designed for magnetic separation, you can also pack this resin in a gravity flow column and use standard chromatography techniques.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I optimize coupling conditions for my protein?\u003c\/strong\u003e\u003cbr\u003eTest different pH (typically 7.0–7.5), temperature (4°C or room temperature), and incubation times (2–18 hours). A small test batch before large-scale coupling is recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a COA or TDS?\u003c\/strong\u003e\u003cbr\u003eYes. Request a quote or contact us and we will provide available lot documentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBarbosa O, Torres R, Ortiz C, Berenguer-Murcia A, Rodrigues RC, Fernandez-Lafuente R. Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties. \u003cem\u003eBiomacromolecules.\u003c\/em\u003e 2013;14(8):2433-62.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/bm400762h\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eNarayanan SR, Kakodkar SV, Crane LJ. \"Glutaraldehyde-P\", a stable, reactive aldehyde matrix for affinity chromatography. \u003cem\u003eAnal Biochem.\u003c\/em\u003e 1990;188(2):278-84.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/0003-2697(90)90606-a\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSuh CW, Choi GS, Lee EK. Enzymic cleavage of fusion protein using immobilized urokinase covalently conjugated to glyoxyl-agarose. \u003cem\u003eBiotechnol Appl Biochem.\u003c\/em\u003e 2003;37(Pt 2):149-55.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1042\/ba20020049\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813554843946,"sku":"BTS-B2025447","price":1245.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025447_e87911f2-33a6-4511-99c2-03e3d6c15082.png?v=1790897685","url":"https:\/\/bluetigerscientific.com\/products\/glyoxal-4bcl-magnetic-1","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}