{"product_id":"glutathione-magnetic-beads","title":"Glutathione Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eGlutathione Magnetic Beads – Catalog #B2026693\u003c\/h2\u003e\n\u003cp\u003eGlutathione Magnetic Beads (Catalog #B2026693) are magnetic particles functionalized with glutathione, the endogenous tripeptide (L-γ-glutamyl-L-cysteinyl-glycine). Supplied as a 2 mL suspension, these beads facilitate rapid, high-efficiency purification of glutathione S-transferase (GST)-tagged fusion proteins and other glutathione-binding proteins through affinity chromatography, batch binding, or magnetic pull-down assays. The integrated magnetic core enables quick separation from solution without centrifugation.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2026693\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight or Concentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCoating:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlutathione (γ-L-glutamyl-L-cysteinyl-glycine)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity purification of GST-tagged proteins, glutathione pull-down assays, protein interaction studies, sample preparation for chromatography\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eGlutathione magnetic beads, GST-tag purification, affinity beads, glutathione affinity, magnetic particle purification, protein pull-down, GST-coated beads, magnetic separation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eGlutathione S-transferases (GSTs) are a large family of enzymes that catalyze the conjugation of the tripeptide glutathione (GSH) to electrophilic substrates. The high affinity between glutathione and GST-tagged fusion proteins makes glutathione-coated surfaces ideal for rapid protein purification. Glutathione magnetic beads combine this affinity interaction with magnetic separation, eliminating the need for gravity flow or centrifugation and dramatically reducing purification time in both batch and column-based workflows.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eRapid batch purification of GST-tagged recombinant proteins\u003c\/li\u003e\n\u003cli\u003eMagnetic bead-based separation suitable for automation\u003c\/li\u003e\n\u003cli\u003ePull-down assays to detect protein-protein interactions\u003c\/li\u003e\n\u003cli\u003eSample preparation and enrichment prior to downstream analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. Mix gently before use to resuspend beads. Before each experiment, wash beads once in bind\/wash buffer and resuspend in the same buffer.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding:\u003c\/strong\u003e Incubate GST-tagged protein with beads at 4°C or room temperature (time depends on protein concentration and desired purity).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Separate beads magnetically and wash 2–4 times with bind\/wash buffer to remove unbound protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e For GST proteins, elute with reduced glutathione (10–20 mM) in bind\/wash buffer, or disrupt beads in SDS-PAGE sample buffer for SDS-PAGE analysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBead recovery:\u003c\/strong\u003e Beads can be regenerated and reused if desired (consult the COA\/TDS for cycle specifications).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e2 mL of glutathione-coated magnetic particles in suspension\u003c\/li\u003e\n\u003cli\u003eReady-to-use for rapid protein purification and sample preparation\u003c\/li\u003e\n\u003cli\u003eMagnetic separation capability for high-throughput and automated workflows\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eRapid purification without centrifugation or gravity flow\u003c\/li\u003e\n\u003cli\u003eHigh binding capacity and specificity for GST-tagged proteins\u003c\/li\u003e\n\u003cli\u003eCompatible with batch, column, and magnetic bead-based separation methods\u003c\/li\u003e\n\u003cli\u003eSuitable for manual or automated high-throughput applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat proteins can I purify with these beads?\u003c\/strong\u003e\u003cbr\u003eAny GST-tagged fusion protein, or any other protein that binds glutathione (e.g., some peroxiredoxins and thioredoxins with glutathione-binding pockets).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I separate the beads from the solution?\u003c\/strong\u003e\u003cbr\u003ePlace the tube in a magnetic stand for 1–2 minutes; beads will collect at the tube wall while liquid can be pipetted away.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse the beads?\u003c\/strong\u003e\u003cbr\u003ePossibly, depending on your protocol and desired purity. Request the COA\/TDS for detailed information on bead stability and reusability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat elution buffer should I use?\u003c\/strong\u003e\u003cbr\u003eReduced glutathione (10–20 mM GSH in appropriate buffer) is the standard choice, though you may also elute by denaturing the sample in SDS-PAGE loading buffer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre the beads suitable for automation?\u003c\/strong\u003e\u003cbr\u003eYes. Magnetic bead-based purification is well-suited to robotic liquid handlers and high-throughput platforms.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a COA or TDS?\u003c\/strong\u003e\u003cbr\u003eYes. Request a quote or contact us and we will provide available lot documentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKielkopf CL, Bauer W, Urbatsch IL. Purification of Fusion Proteins by Affinity Chromatography on Glutathione Resin. \u003cem\u003eCold Spring Harb Protoc.\u003c\/em\u003e 2020;2020(6):102202.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1101\/pdb.prot102202\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eMishra V. Affinity Tags for Protein Purification. \u003cem\u003eCurr Protein Pept Sci.\u003c\/em\u003e 2020;21(8):821-830.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.2174\/1389203721666200606220109\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSchäfer F, Seip N, Maertens B, Block H, Kubicek J. Purification of GST-Tagged Proteins. \u003cem\u003eMethods Enzymol.\u003c\/em\u003e 2015;559:127-39.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/bs.mie.2014.11.005\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813556252970,"sku":"BTS-B2026693","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2026693.png?v=1790897762","url":"https:\/\/bluetigerscientific.com\/products\/glutathione-magnetic-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}