{"product_id":"glass-beads-acid-washed-425-600-um-high-purity","title":"Glass Beads, Acid-Washed (425–600 µm), High Purity","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eGlass Beads, Acid-Washed (425–600 µm), High Purity – Catalog #B2025462\u003c\/h2\u003e\n\u003cp\u003eGlass Beads, Acid-Washed (425–600 µm; Catalog #B2025462) are inert, high-purity spherical glass particles optimized for mechanical disruption of tougher cell types and tissues. Supplied as 25 g of beads, this larger size range is well suited to plant tissues, fungal cell walls, and other samples requiring high shear forces. Acid washing removes surface metal oxides, proteins, and biofilm, ensuring low background contamination in genomic, proteomic, and biochemical downstream analyses.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025462\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e425–600 µm (large)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMaterial:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHigh-purity borosilicate glass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBeads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eRoom temperature, dry conditions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePlant tissue disruption, fungal cell lysis, tough cell wall disruption, enzyme extraction, protein extraction, DNA\/RNA extraction from resistant organisms, sample homogenization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eglass beads, acid-washed glass beads, high-purity beads, cell disruption beads, bead beating, borosilicate glass, tissue disruption, large beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eGlass beads are an established, cost-effective tool for mechanical cell disruption across a broad range of applications. Borosilicate glass is chemically inert and thermally stable, resisting attack from harsh lysis buffers and sustaining repeated use cycles. The 425–600 µm size range is suited to applications requiring greater mechanical force: disrupting plant cell walls (which are tougher than bacterial or yeast cell walls), fungal tissues, and dense or fibrous samples. Larger beads generate higher energy per collision during beating, making them effective for resistant organisms and tissues that would require extended beating times with smaller beads. Acid washing removes adsorbed metal oxides, proteins, and organic contaminants, minimizing background in sensitive assays.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003ePlant tissue and leaf disruption for nucleic acid extraction\u003c\/li\u003e\n\u003cli\u003eFungal cell disruption and protein extraction\u003c\/li\u003e\n\u003cli\u003eTough bacterial cell wall disruption (e.g., gram-positive spore-formers)\u003c\/li\u003e\n\u003cli\u003eTissue homogenization for proteomic analysis\u003c\/li\u003e\n\u003cli\u003eEnzyme and organelle extraction\u003c\/li\u003e\n\u003cli\u003eSample preparation for shotgun sequencing and metagenomics\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eFill a disruption tube to approximately half its volume with beads (the larger size leaves more room for sample than smaller beads). Add your sample in lysis buffer and beat at high speed for 30–120 seconds, adjusting duration based on cell type toughness and equipment power. The larger bead mass generates higher impact energy, so start with shorter beating intervals to avoid excessive sample degradation. For RNA-sensitive work, use RNase-free equipment and cooled buffers.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eTube loading:\u003c\/strong\u003e ~1\/2 to 2\/3 fill with beads, remaining volume for sample; adjust ratio for your tube size and bead capacity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBeating parameters:\u003c\/strong\u003e Typically 4,000–6,000 rpm for 30–120 seconds; optimize for cell type and instrument power.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCooling:\u003c\/strong\u003e Use pre-cooled tubes and buffers or intersperse beating intervals with brief rest periods to prevent sample degradation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReuse:\u003c\/strong\u003e Beads can be reused after thorough washing and complete drying, or autoclaved at ≤200°C before reuse.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 g of high-purity, acid-washed borosilicate glass beads\u003c\/li\u003e\n\u003cli\u003eBeads sized 425–600 µm, optimized for tough plant tissue and fungal cell disruption\u003c\/li\u003e\n\u003cli\u003eLow contamination and minimal metal leaching for sensitive downstream assays\u003c\/li\u003e\n\u003cli\u003eSufficient quantity for extended research or routine processing workflows\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eLarger bead size generates greater mechanical force, reducing beating time for resistant samples\u003c\/li\u003e\n\u003cli\u003eAcid washing minimizes metal and organic background in sensitive assays\u003c\/li\u003e\n\u003cli\u003eInert borosilicate glass tolerates harsh lysis buffers and repeated use\u003c\/li\u003e\n\u003cli\u003eCost-effective reusable option for high-throughput plant, fungal, and tissue work\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do 425–600 µm beads differ from smaller beads?\u003c\/strong\u003e\u003cbr\u003eLarger beads generate more force per collision, reducing beating time for tough samples like plant tissue. They are less efficient for small, soft cells and occupy more tube volume.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long should I beat to get good disruption?\u003c\/strong\u003e\u003cbr\u003eTypical range is 30–120 seconds at 4,000–6,000 rpm, depending on sample toughness and equipment. Start with 30 seconds and extend if needed.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these for RNA work?\u003c\/strong\u003e\u003cbr\u003eYes, use RNase-free tubes and beaters, and keep samples on ice or use cooled buffer and brief beating intervals to preserve RNA.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre the beads reusable?\u003c\/strong\u003e\u003cbr\u003eYes. Wash thoroughly with deionized water, dry completely at room temperature, and optionally autoclave at low temperature (≤200°C) before reuse.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat happens if I beat too long?\u003c\/strong\u003e\u003cbr\u003eExtended beating generates heat and shear stress that can degrade proteins, RNA, and DNA. Optimize beating time for your sample type and monitor for excessive degradation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eDeCaprio J, Kohl TO. Lysing Yeast Cells with Glass Beads for Immunoprecipitation. \u003cem\u003eCold Spring Harb Protoc.\u003c\/em\u003e 2020;2020(11).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1101\/pdb.prot098590\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGarcía-Durán C, Martínez-López R, Zapico I, Pérez E, Romeu E, Arroyo J, Hernáez ML, Pitarch A, Monteoliva L, Gil C. Distinct Human Gut Microbial Taxonomic Signatures Uncovered With Different Sample Processing and Microbial Cell Disruption Methods for Metaproteomic Analysis. \u003cem\u003eFront Microbiol.\u003c\/em\u003e 2021;12:618566.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3389\/fmicb.2021.618566\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHwang KY, Kwon SH, Jung SO, Lim HK, Jung WJ, Park CS, Kim JH, Suh KY, Huh N. Miniaturized bead-beating device to automate full DNA sample preparation processes for gram-positive bacteria. \u003cem\u003eLab Chip.\u003c\/em\u003e 2011;11(21):3649-55.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1039\/c1lc20692c\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813555007786,"sku":"BTS-B2025462","price":1185.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025462.png?v=1790897695","url":"https:\/\/bluetigerscientific.com\/products\/glass-beads-acid-washed-425-600-um-high-purity","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}