{"product_id":"glass-beads-acid-washed-215-300-um-high-purity","title":"Glass Beads, Acid-Washed (215–300 µm), High Purity","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eGlass Beads, Acid-Washed (215–300 µm), High Purity – Catalog #B2025504\u003c\/h2\u003e\n\u003cp\u003eGlass Beads, Acid-Washed (215–300 µm; Catalog #B2025504) are inert, high-purity spherical glass particles designed for mechanical cell disruption in bead beating applications. Supplied as 5 g of beads, these medium-sized particles are optimized for rapid, efficient lysis of bacterial, fungal, yeast, and plant cells, and for tissue disruption in sample preparation workflows. Acid washing removes surface metal oxides, proteins, and biofilm contaminants, ensuring low background contamination in downstream analyses such as DNA and RNA extraction.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025504\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e215–300 µm (medium)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMaterial:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHigh-purity borosilicate glass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBeads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eRoom temperature, dry conditions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCell lysis, bead beating, tissue disruption, DNA\/RNA extraction, bacterial and fungal cell disruption, protein extraction, sample homogenization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eglass beads, acid-washed glass beads, high-purity beads, cell disruption beads, bead beating, borosilicate glass, tissue disruption\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eGlass beads are among the oldest and most effective tools for mechanical cell disruption. Their inert composition, durability, and cost-effectiveness have made them a standard in laboratory automation and high-throughput sample preparation. Borosilicate glass (commonly Corning® Pyrex®) resists chemical attack and thermal shock, ensuring consistent performance across many uses. The 215–300 µm size range offers a balance suitable for small bacterial and fungal cells: large enough to generate effective shear forces during beating but small enough to disrupt tightly walled cells efficiently. Acid washing removes surface contamination, metal oxides, and organic residues that could interfere with enzymatic assays, protein analysis, or nucleic acid quantification.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eBacterial and fungal cell lysis for DNA\/RNA extraction\u003c\/li\u003e\n\u003cli\u003eTissue and plant material disruption\u003c\/li\u003e\n\u003cli\u003eProtein extraction from cells and tissues\u003c\/li\u003e\n\u003cli\u003eSample homogenization in genomics and proteomics workflows\u003c\/li\u003e\n\u003cli\u003eEnzyme and organelle extraction\u003c\/li\u003e\n\u003cli\u003eHigh-throughput sample preparation in automated workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eBeads are used in mechanical disruption devices (bead beaters, tissue homogenizers, or vortex-mounted bead beating tubes). Fill the disruption tube to about two-thirds capacity with beads, add sample (cells in lysis buffer), and beat for 15–60 seconds at high speed, depending on cell type and desired disruption efficiency. For RNA work, use RNase-free beads and beaters. The acid-washed surface helps minimize contamination, but ensure all equipment is clean and sterile for sensitive applications.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eTube loading:\u003c\/strong\u003e ~2\/3 fill with beads, ~1\/3 with sample (adjust for downstream analysis requirements).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBeating parameters:\u003c\/strong\u003e Typically 5,000–7,000 rpm for 15–60 seconds; optimize for your cell type and instrument.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCooling:\u003c\/strong\u003e If not already cooled, use pre-cooled tubes and buffers or add cooling time between beats to prevent sample degradation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReuse:\u003c\/strong\u003e Beads can be reused after thorough washing and drying, or autoclaved if thermal stability is acceptable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 g of high-purity, acid-washed borosilicate glass beads\u003c\/li\u003e\n\u003cli\u003eBeads sized 215–300 µm, optimized for bacterial, fungal, and plant cell disruption\u003c\/li\u003e\n\u003cli\u003eLow contamination and minimal metal leaching for sensitive downstream assays\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eConsistent, reproducible cell disruption across a wide range of sample types\u003c\/li\u003e\n\u003cli\u003eAcid washing reduces background contamination in genomic and proteomic analyses\u003c\/li\u003e\n\u003cli\u003eInert borosilicate glass is compatible with harsh lysis buffers and organic solvents\u003c\/li\u003e\n\u003cli\u003eDurable and reusable, reducing per-sample cost in high-throughput workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much sample disruption will I get with these beads?\u003c\/strong\u003e\u003cbr\u003eDisruption efficiency depends on beating intensity, duration, sample viscosity, and cell wall thickness. Optimize by testing disruption time (typically 15–60 seconds) and rpm with your bead beater and cell type.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these for RNA extraction?\u003c\/strong\u003e\u003cbr\u003eYes, use RNase-free tubes and beaters, and keep samples on ice or in cooled conditions during beating.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan the beads be reused?\u003c\/strong\u003e\u003cbr\u003eYes. Wash thoroughly with deionized water, dry completely at room temperature or in an oven (do not exceed 200°C for extended periods), and autoclave if desired before reuse.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the acid-washing process?\u003c\/strong\u003e\u003cbr\u003eBeads are treated with dilute hydrochloric acid or similar to remove surface metal oxides, proteins, and biofilm, then rinsed and dried.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I store them?\u003c\/strong\u003e\u003cbr\u003eKeep in a dry container at room temperature. Avoid moisture to prevent clumping.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eDeCaprio J, Kohl TO. Lysing Yeast Cells with Glass Beads for Immunoprecipitation. \u003cem\u003eCold Spring Harb Protoc.\u003c\/em\u003e 2020;2020(11).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1101\/pdb.prot098590\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHwang KY, Kwon SH, Jung SO, Lim HK, Jung WJ, Park CS, Kim JH, Suh KY, Huh N. Miniaturized bead-beating device to automate full DNA sample preparation processes for gram-positive bacteria. \u003cem\u003eLab Chip.\u003c\/em\u003e 2011;11(21):3649-55.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1039\/c1lc20692c\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eRoberts AV. The use of bead beating to prepare suspensions of nuclei for flow cytometry from fresh leaves, herbarium leaves, petals and pollen. \u003cem\u003eCytometry A.\u003c\/em\u003e 2007;71(12):1039-44.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/cyto.a.20486\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813555564842,"sku":"BTS-B2025504","price":1235.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025504.png?v=1790897723","url":"https:\/\/bluetigerscientific.com\/products\/glass-beads-acid-washed-215-300-um-high-purity","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}