{"product_id":"glass-beads-acid-washed-105-um-high-purity","title":"Glass Beads, Acid-Washed (≤105 µm), High Purity","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eGlass Beads, Acid-Washed (≤105 µm), High Purity – Catalog #B2025491\u003c\/h2\u003e\n\u003cp\u003eGlass Beads, Acid-Washed (≤105 µm; Catalog #B2025491) are inert, high-purity spherical particles optimized for gentle, controlled disruption of bacterial cells, small yeast, and other microorganisms. Supplied as 25 g of beads, the fine size range (≤105 micrometers) provides high surface area and rapid momentum transfer, enabling efficient cell lysis with minimal sample degradation. Acid washing removes surface metal oxides, proteins, and biofilm contaminants, ensuring exceptionally low background in sensitive downstream applications such as RNA-seq, qPCR, and proteomics.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025491\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e≤105 µm (fine)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMaterial:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHigh-purity borosilicate glass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBeads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eRoom temperature, dry conditions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBacterial cell lysis, small cell disruption, yeast cell disruption, DNA\/RNA extraction, enzyme extraction, protein analysis, sensitive genomic and proteomic studies, microorganism preparation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eglass beads, acid-washed glass beads, high-purity beads, fine glass beads, cell disruption beads, bead beating, borosilicate glass, small beads, microbeads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eFine glass beads (≤105 µm) are particularly suited to bacteria, small yeasts, and other microorganisms that require gentler, more controlled disruption. The smaller bead size allows more beads to occupy the same volume, creating a denser packing that generates both shear stress and crushing forces during beating. This combination enables efficient cell disruption while the high number of contact points distributes energy more uniformly, reducing local hot spots that can denature proteins or degrade nucleic acids. Borosilicate glass is chemically inert and heat-resistant, maintaining performance across repeated beating cycles. Acid washing removes surface oxides and contaminating proteins, providing minimal background interference in quantitative assays such as qPCR, RT-qPCR, and protein quantification.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eBacterial genomic DNA extraction and purification\u003c\/li\u003e\n\u003cli\u003eRNA preparation from microorganisms for expression analysis\u003c\/li\u003e\n\u003cli\u003eMetagenomic and metatranscriptomic sample preparation\u003c\/li\u003e\n\u003cli\u003eEnzyme and protein extraction from bacterial and yeast cells\u003c\/li\u003e\n\u003cli\u003ePreparation of samples for next-generation sequencing (NGS)\u003c\/li\u003e\n\u003cli\u003eSensitive proteomic analyses requiring minimal background\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eFill disruption tubes to approximately two-thirds volume with fine beads (they pack more densely than larger sizes), add sample in lysis buffer, and beat for 15–45 seconds at 5,000–7,000 rpm. The fine bead size requires careful control of beating time: excessively long beating generates heat and excess shear that can damage nucleic acids and proteins. For RNA work, use RNase-free tubes, equipment, and cooled buffers. Start with shorter beating intervals and increase if necessary to avoid over-disruption.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eTube loading:\u003c\/strong\u003e ~2\/3 fill with beads, ~1\/3 with sample; fine beads pack densely, so adjust ratio as needed for your tube geometry.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBeating parameters:\u003c\/strong\u003e Typically 5,000–7,000 rpm for 15–45 seconds; start conservatively and extend beating time if disruption is incomplete.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCooling:\u003c\/strong\u003e Use pre-cooled tubes and buffers; RNA-sensitive work benefits from minimal beating time or cooled equipment.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReuse:\u003c\/strong\u003e Wash thoroughly with ultrapure water, dry completely at room temperature, and optionally autoclave at ≤200°C before reuse.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 g of high-purity, acid-washed borosilicate glass beads\u003c\/li\u003e\n\u003cli\u003eBeads sized ≤105 µm, optimized for bacterial and small cell disruption\u003c\/li\u003e\n\u003cli\u003eHigh surface area and precise size distribution for rapid, uniform disruption\u003c\/li\u003e\n\u003cli\u003eMinimal contaminant background for sensitive downstream assays\u003c\/li\u003e\n\u003cli\u003eSufficient quantity for extensive research or high-throughput workflows\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eFine bead size provides rapid, controlled disruption with less beating time than larger beads\u003c\/li\u003e\n\u003cli\u003eHigh bead density enables efficient cell lysis while preserving nucleic acid and protein integrity\u003c\/li\u003e\n\u003cli\u003eAcid washing ensures low background in qPCR, RT-qPCR, and sensitive protein assays\u003c\/li\u003e\n\u003cli\u003eInert, durable glass beads are compatible with harsh buffers and repeated reuse\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhy use fine beads instead of larger ones?\u003c\/strong\u003e\u003cbr\u003eFine beads pack densely, create more contact points, and transfer energy more uniformly. They are ideal for small bacteria and sensitive work where minimizing beating time prevents sample degradation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long should I beat with fine beads?\u003c\/strong\u003e\u003cbr\u003eTypically 15–45 seconds at 5,000–7,000 rpm. Shorter times than larger beads because energy is distributed more effectively. Start short and extend if disruption is incomplete.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre these suitable for RNA extraction?\u003c\/strong\u003e\u003cbr\u003eYes, and they are preferred for RNA work because the shorter beating time reduces shear stress on RNA. Use RNase-free equipment and cooled buffers.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I prevent over-disruption?\u003c\/strong\u003e\u003cbr\u003eMonitor disruption with a microscope or viability assay. Start with short beating intervals (15 seconds) and increase if cell disruption is incomplete.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse these beads?\u003c\/strong\u003e\u003cbr\u003eYes. Wash with ultrapure water, dry completely, and optionally autoclave at ≤200°C before reuse.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eDeCaprio J, Kohl TO. Lysing Yeast Cells with Glass Beads for Immunoprecipitation. \u003cem\u003eCold Spring Harb Protoc.\u003c\/em\u003e 2020;2020(11).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1101\/pdb.prot098590\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHwang KY, Kwon SH, Jung SO, Lim HK, Jung WJ, Park CS, Kim JH, Suh KY, Huh N. Miniaturized bead-beating device to automate full DNA sample preparation processes for gram-positive bacteria. \u003cem\u003eLab Chip.\u003c\/em\u003e 2011;11(21):3649-55.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1039\/c1lc20692c\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGarcía-Durán C, Martínez-López R, Zapico I, Pérez E, Romeu E, Arroyo J, Hernáez ML, Pitarch A, Monteoliva L, Gil C. Distinct Human Gut Microbial Taxonomic Signatures Uncovered With Different Sample Processing and Microbial Cell Disruption Methods for Metaproteomic Analysis. \u003cem\u003eFront Microbiol.\u003c\/em\u003e 2021;12:618566.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3389\/fmicb.2021.618566\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813555269930,"sku":"BTS-B2025491","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025491.png?v=1790897709","url":"https:\/\/bluetigerscientific.com\/products\/glass-beads-acid-washed-105-um-high-purity","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}