{"product_id":"extrabind-protein-g-agarose-resin","title":"ExtraBind Protein G Agarose Resin","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eExtraBind Protein G Agarose Resin – Catalog #B2025428\u003c\/h2\u003e\n\u003cp\u003eExtraBind Protein G Agarose Resin (Catalog #B2025428) is a pre-packed affinity chromatography resin for high-capacity purification of immunoglobulin G (IgG) and other antibodies from serum, cell culture supernatants, and other biological samples. Protein G, derived from Streptococcus, binds the Fc region of antibodies across a broad range of species, enabling species-independent purification without requiring prior knowledge of antibody source.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025428\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMatrix:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e6% crosslinked agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAntibody purification, immunoglobulin enrichment, antibody screening, polyclonal and monoclonal antibody isolation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eProtein G agarose, Protein G affinity resin, antibody purification resin, IgG purification, immunoglobulin purification, Fc binding resin, ExtraBind resin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eProtein G is a surface protein from Streptococcus (typically S. pyogenes or S. agalactiae) that binds the Fc region of immunoglobulins. Unlike Protein A, which shows species-dependent binding preferences (strong for mouse and human IgG but weak for goat or sheep IgG), Protein G binds broadly across mammalian IgG from most species. The agarose matrix is a cross-linked polysaccharide that provides high physical and chemical stability during repeated use, making it suitable for both gravity-driven and pressure-driven column chromatography. The 6% crosslinking offers a balance between flow rate and mechanical strength.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003ePurification of polyclonal antibodies from animal sera\u003c\/li\u003e\n\u003cli\u003eAffinity enrichment of monoclonal antibodies from cell culture supernatants\u003c\/li\u003e\n\u003cli\u003eRemoval of non-specific immunoglobulins from biological samples\u003c\/li\u003e\n\u003cli\u003ePreparation of purified antibodies for diagnostic assays, Western blotting, and immunoprecipitation\u003c\/li\u003e\n\u003cli\u003eAntibody screening and characterization\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eThe resin can be used in gravity-flow columns or with peristaltic pump systems. Equilibrate with 5–10 column volumes of binding buffer (typically 0.1 M phosphate or 0.1 M Tris, pH 7–8) before loading. Load sample at 1–4 mL\/min per mL of resin and collect flowthrough if non-IgG proteins are to be recovered separately. Elute bound antibodies with a mild elution buffer (pH 2.5–3.0, such as 0.1 M citric acid or glycine-HCl) collected into neutralization buffer. The resin can be regenerated with 0.1 M glycine-HCl pH 2.5 for 5–10 minutes, then re-equilibrated.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eLoading:\u003c\/strong\u003e Apply 0.1–10 mg IgG per mL of resin, depending on sample composition and purity requirements.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow rate:\u003c\/strong\u003e 1–4 mL\/min per mL of resin for typical column diameters.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Use pH 2.5–3.0 buffers; collect in neutralization buffer to immediately restore pH.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Keep packed resin at 2–8°C in 20% ethanol or storage buffer supplied by the manufacturer.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of Protein G agarose resin, pre-packed or as a slurry\u003c\/li\u003e\n\u003cli\u003eHigh binding capacity for IgG across multiple species\u003c\/li\u003e\n\u003cli\u003eStable 6% crosslinked agarose matrix for repeated use\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eSpecies-independent Fc binding covers antibodies from mouse, human, goat, sheep, rabbit, and other mammals\u003c\/li\u003e\n\u003cli\u003eHigh capacity and rapid flow rate for large-scale antibody purification\u003c\/li\u003e\n\u003cli\u003eStable agarose matrix withstands pH extremes and organic solvents, allowing regeneration and reuse\u003c\/li\u003e\n\u003cli\u003eMild elution conditions preserve antibody structure and biological activity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between Protein G and Protein A?\u003c\/strong\u003e\u003cbr\u003eBoth bind Fc regions, but Protein A shows strong affinity for human and mouse IgG, while Protein G binds more broadly across mammalian species, including goat and sheep antibodies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much sample can I load?\u003c\/strong\u003e\u003cbr\u003eTypically 1–10 mg IgG per mL of resin, depending on purity. Request a COA\/TDS for lot-specific binding capacity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat pH should I use for elution?\u003c\/strong\u003e\u003cbr\u003epH 2.5–3.0 is standard; collect into neutralization buffer immediately to restore pH and preserve antibody activity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan the resin be reused?\u003c\/strong\u003e\u003cbr\u003eYes. Regenerate by washing with pH 2.5–3.0 buffer for 5–10 minutes, then re-equilibrate with binding buffer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I set up the column?\u003c\/strong\u003e\u003cbr\u003ePour slurry into a gravity or pump column, allow settling, equilibrate with binding buffer, and begin loading your sample.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eCassedy A, O'Kennedy R. Antibody Purification Using Affinity Chromatography. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2022;2466:3-22.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-2176-9_1\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGrodzki AC, Berenstein E. Antibody purification: affinity chromatography - protein A and protein G Sepharose. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2010;588:33-41.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-59745-324-0_5\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eFishman JB, Berg EA. Protein A and Protein G Purification of Antibodies. \u003cem\u003eCold Spring Harb Protoc.\u003c\/em\u003e 2019;2019(1).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1101\/pdb.prot099143\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eDarcy E, Leonard P, Fitzgerald J, Danaher M, Ma H, O'Kennedy R. Purification of Antibodies Using Affinity Chromatography. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2017;1485:305-318.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-4939-6412-3_15\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813554680106,"sku":"BTS-B2025428","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025428.png?v=1790897679","url":"https:\/\/bluetigerscientific.com\/products\/extrabind-protein-g-agarose-resin","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}