{"product_id":"epoxy-magnetic-beads-1-um-2","title":"Epoxy Magnetic Beads (1 µm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eEpoxy Magnetic Beads (1 µm) – Catalog #B2025679\u003c\/h2\u003e\n\u003cp\u003eEpoxy Magnetic Beads (1 µm; Catalog #B2025679) are superparamagnetic particles functionalized with epoxy groups that form stable covalent bonds with lysine residues of proteins and ligands. Supplied as a 1 mL suspension, these 1-micrometer beads combine the reactivity of epoxy chemistry with the convenience of magnetic separation, enabling rapid sample processing and high recovery in affinity-based applications.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025679\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity capture, immunoassays, immunoprecipitation, protein purification, antibody coupling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eepoxy magnetic beads, magnetic particles, affinity beads, protein coupling beads, immunomagnetic beads, epoxy-functionalized beads, superparamagnetic beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eEpoxy-functionalized magnetic beads are widely used for immunoassays and affinity purification because epoxy groups form stable, covalent bonds with primary amines (lysine and N-terminal residues) on proteins and ligands. The superparamagnetic iron oxide core allows beads to be rapidly separated from solution using a magnet, without requiring centrifugation. At 1 µm, these beads offer a favorable balance: large enough for efficient magnetic capture, small enough to provide high surface area and rapid diffusion kinetics.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eCovalent coupling of antibodies, enzymes, and affinity ligands\u003c\/li\u003e\n\u003cli\u003eImmunomagnetic separation and immunoprecipitation\u003c\/li\u003e\n\u003cli\u003eProtein purification and sample preparation\u003c\/li\u003e\n\u003cli\u003eHigh-throughput immunoassays and bead-based diagnostics\u003c\/li\u003e\n\u003cli\u003eAffinity enrichment of target molecules from complex samples\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C; shake gently before use to ensure homogeneous suspension. Epoxy groups react with primary amines under slightly alkaline conditions (pH 8–10). Optimize pH and temperature for coupling (typically room temperature to 4°C overnight). After coupling, incubate unused epoxy groups with an amine buffer (e.g., ethanolamine or glycine) to reduce nonspecific binding.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling protocol:\u003c\/strong\u003e Incubate beads with your protein or ligand at pH 8–10 for 2–16 hours; coupling efficiency depends on amine concentration and pH.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking:\u003c\/strong\u003e Quench remaining epoxy groups with a blocking amine to reduce nonspecific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Wash beads magnetically using a magnet to retain beads while removing solution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store coupled beads at 2–8°C; refer to your blocking and storage buffer for optimal stability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 mL suspension of 1 µm epoxy magnetic beads\u003c\/li\u003e\n\u003cli\u003eReactive epoxy groups for direct protein coupling\u003c\/li\u003e\n\u003cli\u003eMagnetic core for rapid separation without centrifugation\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eStable covalent bonds reduce nonspecific binding compared to passive adsorption\u003c\/li\u003e\n\u003cli\u003e1 µm size balances surface area with efficient magnetic separation\u003c\/li\u003e\n\u003cli\u003eDirect coupling of antibodies, enzymes, and ligands without intermediate linkers\u003c\/li\u003e\n\u003cli\u003eHigh recovery and minimal sample loss in magnetic separation workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do epoxy beads differ from carboxyl- or amino-functionalized beads?\u003c\/strong\u003e\u003cbr\u003eEpoxy groups react with amines to form stable secondary amines (not reversible), whereas carboxyl or amino beads often use intermediate linkers or salt-dependent binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the optimal pH for coupling?\u003c\/strong\u003e\u003cbr\u003epH 8–10 is typical; the exact pH depends on your protein and buffer. Request a COA\/TDS for lot-specific recommendations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long does coupling take?\u003c\/strong\u003e\u003cbr\u003eTypically 2–16 hours at room temperature or 4°C, depending on amine concentration and pH.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse the beads?\u003c\/strong\u003e\u003cbr\u003eOnce a protein is covalently coupled, the beads are generally not reusable. Plan quantities accordingly.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I prevent nonspecific binding?\u003c\/strong\u003e\u003cbr\u003eBlock unused epoxy groups with a primary amine (glycine or ethanolamine) and use appropriate washing buffers to reduce nonspecific adsorption.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eCao R, Xu S, Yu Z, Xu L, Ge Z, Huo Q, Zhu G, Qiao B. Integration of protein L-immobilized epoxy magnetic bead capture with LC-MS\/MS for therapeutic monoclonal antibody quantification in serum. \u003cem\u003eAnal Methods.\u003c\/em\u003e 2024;16(23):3720-3731.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1039\/d4ay00433g\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHaukanes BI, Kvam C. Application of magnetic beads in bioassays. \u003cem\u003eBiotechnology (N Y).\u003c\/em\u003e 1993;11(1):60-3.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1038\/nbt0193-60\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eZhu G, Walter JG. Aptamer-modified magnetic beads in affinity separation of proteins. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2015;1286:67-82.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-4939-2447-9_7\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813555958058,"sku":"BTS-B2025679","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025679_9562092e-80a2-4863-9584-046aa3851a5c.png?v=1790897735","url":"https:\/\/bluetigerscientific.com\/products\/epoxy-magnetic-beads-1-um-2","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}