{"product_id":"epoxy-magnetic-agarose-beads","title":"Epoxy Magnetic Agarose Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eEpoxy Magnetic Agarose Beads – Catalog #B2025489\u003c\/h2\u003e\n\u003cp\u003eEpoxy Magnetic Agarose Beads are a versatile affinity chromatography medium combining several advantages: a porous agarose matrix for high binding capacity, epoxy (oxirane) surface groups for efficient covalent coupling of ligands, and a magnetic iron oxide core that enables rapid, easy separation from solution. The epoxy groups are highly reactive with amino, hydroxyl, and thiol groups on proteins, enzymes, antibodies, carbohydrates, and other biomolecules, forming stable ether linkages and secondary amine bonds under mildly alkaline conditions. This allows researchers to quickly immobilize their own ligands (capture antibodies, enzymes, receptors, or other targets) and create customized affinity purification columns or immunoassay platforms.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025489\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution (bead suspension)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMatrix:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAgarose (porous, high-capacity)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eFunctional groups:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eEpoxy (oxirane)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSpecial feature:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic iron oxide core for easy separation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity chromatography with custom ligands, enzyme immobilization, antibody purification platforms, immunoassay matrices, biomarker capture, protein interaction studies, functional proteomics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eEpoxy magnetic beads, epoxy agarose beads, magnetic agarose, epoxy-functionalized magnetic beads, ligand immobilization, affinity chromatography, enzyme immobilization, protein coupling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eEpoxy groups (also called oxirane or glycidyl groups) are highly reactive functional moieties commonly used in organic and biochemistry for covalent coupling reactions. Under mildly alkaline conditions (typically pH 8–11), epoxy groups react spontaneously with primary amines (–NH₂) on proteins and peptides, forming stable secondary amine linkages. They also react more slowly with hydroxyl (–OH) and thiol (–SH) groups, although amino coupling is preferred. The key advantage of epoxy-functionalized beads is that coupling occurs under gentle conditions without requiring additional chemical cross-linkers, making the process straightforward: mix the ligand (protein, antibody, enzyme) with the epoxy beads at pH 8–11, incubate, and the ligand is covalently immobilized.\u003c\/p\u003e\n\u003cp\u003eThe combination of epoxy coupling chemistry, a high-capacity agarose matrix, and magnetic functionality makes these beads ideal for:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eBuilding custom affinity columns with your own specific ligands (antibodies, enzymes, receptors)\u003c\/li\u003e\n\u003cli\u003eCreating immobilized enzyme reactors (IMERs) for biocatalysis and analytical applications\u003c\/li\u003e\n\u003cli\u003eRapid immunoassay platforms (e.g., magnetic bead-based sandwich assays)\u003c\/li\u003e\n\u003cli\u003eBiomarker capture and sample enrichment in complex biological samples\u003c\/li\u003e\n\u003cli\u003eProtein interaction and pull-down studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eEpoxy coupling is simple and robust. Pre-treat the beads by rinsing in a slightly alkaline coupling buffer (pH 8–11; sodium carbonate or sodium phosphate buffers are common). Mix the target ligand (protein or antibody) at 1–10 mg\/mL with the epoxy beads in coupling buffer and incubate at room temperature or 4°C for 2–24 hours. The longer incubation and mildly elevated pH improve coupling efficiency. After coupling, wash the beads with coupling buffer, then neutralize and store in a neutral pH buffer (PBS or Tris-HCl, pH 7.2–7.4).\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling buffer:\u003c\/strong\u003e Sodium carbonate (pH 9–10) or sodium phosphate (pH 8–9) buffers work well. Avoid buffers containing primary amines (e.g., Tris), which compete with your ligand for coupling.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLigand concentration:\u003c\/strong\u003e Typically 1–10 mg\/mL protein in coupling buffer. Higher protein concentrations often give more efficient coupling, but use the minimum needed for your application to reduce cost.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling time:\u003c\/strong\u003e 2–4 hours at room temperature or overnight at 4°C. Longer incubations improve coupling efficiency.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking unreacted epoxy groups:\u003c\/strong\u003e After coupling, unreacted epoxy groups can be blocked by incubation in ethanolamine or glycine solution (pH 8–9) to prevent non-specific binding; skip this step if you want to couple additional ligands.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage of functionalized beads:\u003c\/strong\u003e Store in neutral pH buffer (PBS or 10 mM Tris-HCl, pH 7.2–7.4) containing 0.02% sodium azide as a bacteriostat, at 2–8°C. Do not freeze.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of epoxy-functionalized magnetic agarose bead suspension\u003c\/li\u003e\n\u003cli\u003eCoupled with the magnetic iron oxide core for rapid magnetic separation\u003c\/li\u003e\n\u003cli\u003eReady for covalent immobilization of your ligand (antibody, enzyme, receptor, etc.)\u003c\/li\u003e\n\u003cli\u003eHigh capacity and biological compatibility\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eStraightforward epoxy-amine coupling chemistry with no additional cross-linkers\u003c\/li\u003e\n\u003cli\u003eHigh-capacity agarose matrix provides good loading and binding\u003c\/li\u003e\n\u003cli\u003eMagnetic properties allow rapid, easy separation and automation\u003c\/li\u003e\n\u003cli\u003eGentle conditions preserve ligand activity and structure\u003c\/li\u003e\n\u003cli\u003eCustomizable: you control which ligand is immobilized\u003c\/li\u003e\n\u003cli\u003eVersatile platform for affinity purification, enzyme immobilization, immunoassays, and interaction studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do epoxy groups couple to my protein?\u003c\/strong\u003e\u003cbr\u003eEpoxy groups react with primary amino groups (lysine residues and the protein's N-terminus) under mildly alkaline pH (8–11), forming stable secondary amine linkages. The reaction is spontaneous; no additional reagents are needed.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat coupling buffer should I use?\u003c\/strong\u003e\u003cbr\u003eSodium carbonate (pH 9–10) or sodium phosphate (pH 8–9) buffers work well. Avoid Tris and other primary amine buffers, which compete with your protein for coupling.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long should I incubate?\u003c\/strong\u003e\u003cbr\u003e2–4 hours at room temperature or overnight at 4°C. Overnight incubation typically gives the best coupling efficiency.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I separate the beads after coupling?\u003c\/strong\u003e\u003cbr\u003eUse a handheld magnet or a magnetic separator rack. Beads will accumulate at the side or bottom within 1–2 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShould I block unreacted epoxy groups?\u003c\/strong\u003e\u003cbr\u003eYes, if you are performing affinity capture assays; incubate with ethanolamine or glycine (pH 8–9) to prevent non-specific binding. Skip blocking if you plan to couple additional ligands.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I store the functionalized beads?\u003c\/strong\u003e\u003cbr\u003eIn neutral pH buffer (PBS, pH 7.2–7.4) with 0.02% sodium azide at 2–8°C. Do not freeze.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eYuan D, Li S, Zhang L, Ma F, Wang H, Zhang Q, Li P. Rapid and sensitive quantification of capsaicinoids for edible oil adulteration by immunomagnetic solid-phase extraction coupled with time-resolved fluorescent immunochromatographic assay. \u003cem\u003eFood Chem.\u003c\/em\u003e 2023;404(Pt A):134552.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.foodchem.2022.134552\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKinumi T, Mizuno R, Takatsu A. Quantification of serum C-peptide by isotope-dilution liquid chromatography-tandem mass spectrometry: enhanced detection using chemical modification and immunoaffinity purification. \u003cem\u003eJ Chromatogr B Analyt Technol Biomed Life Sci.\u003c\/em\u003e 2014;953-954:138-42.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jchromb.2014.02.019\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813555171626,"sku":"BTS-B2025489","price":1225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025489.png?v=1790897704","url":"https:\/\/bluetigerscientific.com\/products\/epoxy-magnetic-agarose-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}