{"product_id":"dna-size-selection-magnetic-beads-1","title":"DNA Size Selection Magnetic Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eDNA Size Selection Magnetic Beads – Catalog #B2025498\u003c\/h2\u003e\n\u003cp\u003eDNA Size Selection Magnetic Beads are carboxyl-functionalized magnetic particles that enable rapid, size-based fractionation of DNA without the use of agarose gels or lengthy column chromatography. These beads bind double-stranded DNA through electrostatic interactions and bead-to-bead bridging, with binding affinity that varies as a function of DNA fragment size: smaller fragments (\u0026lt; ~300 bp) bind weakly, intermediate fragments (300–2,000 bp) bind moderately, and larger fragments (\u0026gt; 2,000 bp) bind strongly. By adjusting the bead concentration and incubation time, researchers can select DNA fragments within a specific size window. The magnetic beads are then pulled from solution using a handheld magnet or automated magnetic separator, allowing rapid recovery of size-selected DNA.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025498\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSolution (magnetic particle suspension)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle composition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCarboxyl-coated magnetic beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eDNA library size selection, NGS library preparation, PCR product purification, DNA fractionation, sample cleanup, high-throughput genomics applications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eDNA size selection beads, magnetic DNA purification, DNA size fractionation, size-select magnetic beads, NGS bead purification, library prep beads, DNA cleanup beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eDNA size selection is a critical step in many genomic workflows, particularly in next-generation sequencing (NGS) library preparation. Traditional methods—such as agarose gel electrophoresis or column chromatography—are time-consuming and often result in DNA loss or shearing. Magnetic bead-based size selection offers a faster, more scalable alternative. Carboxyl-coated magnetic beads bind DNA through electrostatic attraction (phosphate backbone to positively charged bead surfaces) and bead-to-bead bridging interactions. The strength of binding increases with DNA fragment length: very short fragments (\u0026lt;\u0026lt; 300 bp) are poorly retained, while long fragments bind tightly. By applying the beads at a controlled concentration and allowing binding to reach equilibrium, researchers can selectively capture fragments within a desired size range. Magnetic separation then pulls the beads (and bound DNA) out of solution rapidly.\u003c\/p\u003e\n\u003cp\u003eSize selection is commonly used to remove:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eAdapter dimers and other contaminants from library preparations\u003c\/li\u003e\n\u003cli\u003eFragments outside a desired size range for targeted sequencing\u003c\/li\u003e\n\u003cli\u003eIncomplete PCR products or primer dimers\u003c\/li\u003e\n\u003cli\u003eSalts, proteins, and other soluble impurities\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eBefore use, resuspend the magnetic beads thoroughly by vortexing or pipette mixing; they settle quickly, so mix immediately before drawing into a pipette. For size selection, combine the bead suspension with DNA in a buffer (typically a TE or Tris-EDTA buffer), incubate to allow binding, then use a magnetic separator or handheld magnet to pull the beads from solution. DNA is then recovered by resuspending the bead pellet in elution buffer and separating again.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eResuspension:\u003c\/strong\u003e Vortex vigorously for 30 seconds to fully resuspend beads. They settle rapidly, so work quickly after resuspension.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBead concentration:\u003c\/strong\u003e The ratio of beads to DNA, measured as a volume ratio (μL beads per μL sample), determines the size cutoff. Consult application notes or protocol guidelines for the specific size range desired (e.g., 0.6× beads to select \u0026gt;300 bp; 0.2× to select \u0026gt;1,000 bp, etc.).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncubation:\u003c\/strong\u003e Allow 5–15 minutes for binding equilibrium at room temperature.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic separation:\u003c\/strong\u003e Use a handheld magnet or magnetic rack; beads will collect at the side or bottom of the tube within 1–2 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Resuspend beads in TE or water, separate magnetically again, and collect supernatant (which now contains the size-selected DNA).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage of beads:\u003c\/strong\u003e Keep at 2–8°C. Do not freeze; freezing can damage the magnetic core and reduce bead function.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 mL of carboxyl-coated magnetic bead suspension\u003c\/li\u003e\n\u003cli\u003eOptimized for DNA fragment selection in the 100 bp to \u0026gt;10,000 bp range\u003c\/li\u003e\n\u003cli\u003eReady to use with handheld magnet or automated magnetic separator\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eFast, high-throughput alternative to gel or column-based size selection\u003c\/li\u003e\n\u003cli\u003eMinimal DNA loss compared to traditional purification methods\u003c\/li\u003e\n\u003cli\u003eCompatible with standard lab equipment (magnetic separators, standard microtubes)\u003c\/li\u003e\n\u003cli\u003eScalable from benchtop to fully automated high-throughput workflows\u003c\/li\u003e\n\u003cli\u003eNo toxic organic solvents or lengthy incubations required\u003c\/li\u003e\n\u003cli\u003eParticularly valuable for NGS library prep and genomics applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do these beads select DNA by size?\u003c\/strong\u003e\u003cbr\u003eCarboxyl-coated beads bind DNA through electrostatic and bridging interactions. Smaller fragments bind weakly; larger fragments bind strongly. By adjusting bead concentration, you set a size threshold.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat size range can I select?\u003c\/strong\u003e\u003cbr\u003eThe specific range depends on bead concentration and incubation conditions. Common applications select fragments \u0026gt;300 bp, \u0026gt;1,000 bp, or with a specific upper size limit (e.g., 300–3,000 bp). Consult the protocol or contact us for guidance on your target size range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use a handheld magnet?\u003c\/strong\u003e\u003cbr\u003eYes. A neodymium or other strong magnet held near the tube will pull beads to the side within 1–2 minutes. For higher throughput, a magnetic separator rack designed for magnetic beads is more efficient.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDo the beads need to be resuspended before each use?\u003c\/strong\u003e\u003cbr\u003eYes. Beads settle rapidly. Vortex thoroughly for 30 seconds immediately before use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the yield of DNA recovery?\u003c\/strong\u003e\u003cbr\u003eTypical recovery is \u0026gt;90% for properly selected size ranges. Recovery may be lower if you select too extreme a size cutoff or use beads at suboptimal concentrations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I freeze the beads to store them?\u003c\/strong\u003e\u003cbr\u003eNo. Freezing can damage the magnetic iron oxide core and render beads ineffective. Store at 2–8°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eCummins PM, Rochfort KD, O'Connor BF. Ion-Exchange Chromatography: Basic Principles and Application. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2017;1485:209-223.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-4939-6412-3_11\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eWallace RG, Rochfort KD. Ion-Exchange Chromatography: Basic Principles and Application. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2023;2699:161-177.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-3362-5_9\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813555433770,"sku":"BTS-B2025498","price":1235.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025498.png?v=1790897719","url":"https:\/\/bluetigerscientific.com\/products\/dna-size-selection-magnetic-beads-1","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}