{"product_id":"deae-agarose-bead","title":"DEAE Agarose Bead","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eDEAE Agarose Bead – Catalog #B2025404\u003c\/h2\u003e\n\u003cp\u003eDEAE (diethylaminoethyl) Agarose Beads are a widely used anion-exchange chromatography medium for the separation and purification of biomolecules based on their charge. The resin consists of a high-purity, porous agarose matrix derivatized with positively charged diethylamino groups. When a sample of negatively charged molecules (proteins, peptides, DNA, RNA, or other anionic species) is applied to the column in a low-salt buffer, the molecules bind through electrostatic interactions. Elution is achieved by increasing salt concentration or adjusting pH to disrupt the electrostatic interactions.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025404\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eResin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMatrix:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAgarose, 4–6% bead size\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eFunctional group:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eDiethylaminoethyl (DEAE), positively charged\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eChromatography type:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAnion-exchange (weak ion-exchanger)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eProtein purification, peptide separation, nucleic acid purification, sample cleanup, two-step purification workflows, biomolecule fractionation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eRoom temperature (RT)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eDEAE agarose, DEAE resin, anion-exchange agarose, ion-exchange chromatography resin, protein purification media, biomolecule separation, DEAE agarose matrix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eIon-exchange chromatography is one of the most powerful and widely used methods for protein and biomolecule purification. DEAE agarose is a weak anion exchanger; the positively charged diethylamino groups (pKa ~9–11) are ionized at neutral pH and bind negatively charged molecules (proteins, peptides, nucleic acids). Binding is reversible: in low-salt buffer (e.g., 10–50 mM Tris or phosphate, pH 7–8), most proteins bind; increasing salt concentration (e.g., a NaCl or KCl gradient to 0.5–1 M) disrupts electrostatic interactions and elutes proteins in order of increasing net negative charge. The porous agarose matrix provides high capacity, fast flow rates, and good recovery of native proteins.\u003c\/p\u003e\n\u003cp\u003eDEAE agarose is particularly valuable in two-step purification schemes: a quick initial anion-exchange step (often in batch mode for high throughput) can remove many contaminants before a second, more selective chromatography or crystallization step.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eInitial purification of heterologous proteins from cell lysates and inclusion bodies\u003c\/li\u003e\n\u003cli\u003eSeparation of isoforms and genetic variants with different charge\u003c\/li\u003e\n\u003cli\u003eNucleic acid (DNA, RNA, oligonucleotide) purification and separation\u003c\/li\u003e\n\u003cli\u003ePeptide fractionation\u003c\/li\u003e\n\u003cli\u003eSample cleanup and buffer exchange\u003c\/li\u003e\n\u003cli\u003ePolishing steps in multi-stage purification workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eDEAE agarose is most effective in buffers at pH 6–9 (neutral to slightly alkaline); the diethylamino groups are not charged at very low pH (\u0026lt; 5) and are maximally charged at higher pH. Equilibrate the resin in the starting buffer before applying the sample. For batch purification, mix the resin with sample, incubate, and centrifuge to separate resin and supernatant. For column chromatography, pack the resin into a column, equilibrate, load sample, wash, and elute with a salt gradient.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBuffer pH:\u003c\/strong\u003e 6–9 is optimal; avoid pH \u0026lt;5 (charges loss) and pH \u0026gt;10 (resin degradation).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow rate:\u003c\/strong\u003e Typical flow rates are 1–5 mL\/min per cm² of column cross-section; do not exceed the specified maximum to avoid bed compression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEquilibration:\u003c\/strong\u003e Pass at least 5 column volumes (CV) of starting buffer through the column or resin before sample application.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution gradient:\u003c\/strong\u003e A linear NaCl or KCl gradient (0–0.5 or 0–1 M over 10–20 CV) is typical; adjust based on binding strength and desired resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Keep the resin at room temperature in buffer containing 0.02% sodium azide (as a bacteriostat). Do not freeze.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 mL of DEAE agarose resin, anion-exchange chromatography medium\u003c\/li\u003e\n\u003cli\u003eHigh-purity agarose matrix with optimized bead size for good flow and capacity\u003c\/li\u003e\n\u003cli\u003eReady to use in columns or batch mode\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eProven, classical anion exchanger with decades of use in protein chemistry\u003c\/li\u003e\n\u003cli\u003eGentle on proteins; maintains native structure and activity during purification\u003c\/li\u003e\n\u003cli\u003eHigh capacity and flow rate relative to other ion-exchange resins\u003c\/li\u003e\n\u003cli\u003eCompatible with all standard chromatography hardware and workflows\u003c\/li\u003e\n\u003cli\u003eCost-effective for routine protein purification\u003c\/li\u003e\n\u003cli\u003eSimple, predictable binding and elution behavior\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat pH should I use?\u003c\/strong\u003e\u003cbr\u003epH 7–8 is typical for protein purification. DEAE is positively charged and most active in the pH 6–9 range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I elute my protein?\u003c\/strong\u003e\u003cbr\u003eIncrease salt concentration (NaCl or KCl) in a gradient from ~10 mM to 0.5–1 M. Proteins elute in order of increasing net negative charge.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use DEAE in batch mode?\u003c\/strong\u003e\u003cbr\u003eYes. Mix resin and sample, incubate (5–30 minutes), centrifuge to pellet resin, and collect supernatant. This is fast and useful for large-scale initial cleanup.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the maximum flow rate?\u003c\/strong\u003e\u003cbr\u003eTypical maximum is 5 mL\/min per cm² of column cross-section. Exceeding this can cause bed compression and poor resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long can the resin be stored?\u003c\/strong\u003e\u003cbr\u003eIndefinitely at room temperature in buffer with 0.02% sodium azide as a bacteriostat. Do not freeze.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eCummins PM, Rochfort KD, O'Connor BF. Ion-Exchange Chromatography: Basic Principles and Application. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2017;1485:209-223.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-4939-6412-3_11\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eWallace RG, Rochfort KD. Ion-Exchange Chromatography: Basic Principles and Application. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 2023;2699:161-177.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/978-1-0716-3362-5_9\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eBollag DM. Ion-exchange chromatography. \u003cem\u003eMethods Mol Biol.\u003c\/em\u003e 1994;36:11-22.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1385\/0-89603-274-4:11\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813554647338,"sku":"BTS-B2025404","price":1195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025404.png?v=1790897674","url":"https:\/\/bluetigerscientific.com\/products\/deae-agarose-bead","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}