{"product_id":"cys-tat-47-57-cell-penetrating-peptide","title":"Cys-TAT (47-57) Cell-Penetrating Peptide","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eCys-TAT (47-57) Cell-Penetrating Peptide – Catalog #B2026688\u003c\/h2\u003e\n\u003cp\u003eCys-TAT (47-57) is a peptide fragment from the HIV-1 TAT (transactivator of transcription) protein, spanning amino acids 47–57, with an N-terminal cysteine residue for chemical conjugation. This arginine-rich sequence is a prototypical cell-penetrating peptide (CPP) that crosses cell membranes efficiently and is widely used to deliver molecular cargo—proteins, peptides, oligonucleotides, and nanoparticles—into living cells.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2026688\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular Weight:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1661.99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eHIV-1 TAT protein, amino acids 47–57\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCell-penetrating peptide carrier, intracellular delivery of proteins and cargo, cell membrane transduction studies, drug delivery research\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCys-TAT, TAT peptide, cell-penetrating peptide, CPP, transactivator peptide, HIV TAT, HIV-1 TAT (47-57), protein transduction, membrane-permeant peptide, cysteine-TAT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eThe TAT protein from HIV-1 is a regulatory protein essential for viral transcription. The minimal cell-penetrating domain spans approximately amino acids 47–57, a sequence rich in positively charged arginine residues. This short motif, often called the TAT peptide or TAT-peptide, is one of the most studied CPPs; it crosses plasma membranes via macropinocytosis and other endocytic pathways and can traffic to the nucleus. The cysteine residue at the N terminus permits covalent attachment of payloads via thiol-reactive chemistry (e.g., maleimide coupling, disulfide formation).\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eIntracellular delivery of proteins, peptides, and enzymes\u003c\/li\u003e\n\u003cli\u003eCell-penetrating vector for therapeutic oligonucleotides and siRNA\u003c\/li\u003e\n\u003cli\u003eNanoparticle targeting and cellular uptake enhancement\u003c\/li\u003e\n\u003cli\u003eTransduction domain for research and tool proteins\u003c\/li\u003e\n\u003cli\u003eMechanistic studies of cell membrane permeability and endocytosis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eResuspend the lyophilized powder in water or physiological buffer (PBS, HBSS, culture medium) immediately before use. TAT peptides are stable over a range of pH and ionic strength but are sensitive to proteolysis in serum and cell culture media; if studying cellular uptake, include protease inhibitors or use serum-free conditions. The cysteine can be oxidized to a disulfide dimer under oxidizing conditions or conjugated to thiol-reactive molecules (e.g., maleimide-activated carriers, activated esters).\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Dissolve in sterile water or buffer to desired concentration (μM to mM range typical for cell studies).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugation:\u003c\/strong\u003e Use maleimide or other thiol-reactive chemistry to couple payloads to the N-terminal cysteine.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Request a lot-specific COA\/TDS for amino acid composition and HPLC purity data.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 mg of Cys-TAT (47-57) peptide, supplied as lyophilized powder\u003c\/li\u003e\n\u003cli\u003eMolecular weight 1661.99 Da\u003c\/li\u003e\n\u003cli\u003eCysteine-functionalized for payload conjugation\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003ePrototypical cell-penetrating peptide with decades of literature validation\u003c\/li\u003e\n\u003cli\u003eEfficient, non-toxic membrane crossing via endocytosis and other pathways\u003c\/li\u003e\n\u003cli\u003eN-terminal cysteine enables site-specific conjugation chemistry\u003c\/li\u003e\n\u003cli\u003eWorks in diverse cell types and delivery contexts\u003c\/li\u003e\n\u003cli\u003eSimple, cost-effective alternative to viral vectors for intracellular delivery\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow does TAT cross cell membranes?\u003c\/strong\u003e\u003cbr\u003eTAT enters cells primarily via energy-dependent endocytosis (macropinocytosis) and can escape into the cytoplasm and traffic to the nucleus.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I conjugate a cargo to the cysteine?\u003c\/strong\u003e\u003cbr\u003eYes. Use maleimide-activated carriers, disulfide coupling, or other thiol-reactive chemistry to attach your payload.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much peptide do I need?\u003c\/strong\u003e\u003cbr\u003eStart with 1–10 μM in your assay; some applications use up to 100 μM. Optimize for your cell type and cargo.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIs this TAT peptide affected by serum?\u003c\/strong\u003e\u003cbr\u003eTAT is sensitive to protease degradation in serum and cell culture medium. Use serum-free conditions or protease inhibitors if studying cellular uptake over time.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I store the lyophilized powder?\u003c\/strong\u003e\u003cbr\u003eAt −20°C or below, protected from moisture. Once reconstituted, aliquot and store at −20°C or keep on ice during use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSchmidt N, Mishra A, Lai GH, Wong GC. Arginine-rich cell-penetrating peptides. \u003cem\u003eFEBS Lett.\u003c\/em\u003e 2010;584(9):1806-13.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.febslet.2009.11.046\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eZou L, Peng Q, Wang P, Zhou B. Progress in Research and Application of HIV-1 TAT-Derived Cell-Penetrating Peptide. \u003cem\u003eJ Membr Biol.\u003c\/em\u003e 2017;250(2):115-122.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/s00232-016-9940-z\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eRizzuti M, Nizzardo M, Zanetta C, Ramirez A, Corti S. Therapeutic applications of the cell-penetrating HIV-1 Tat peptide. \u003cem\u003eDrug Discov Today.\u003c\/em\u003e 2015;20(1):76-85.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.drudis.2014.09.017\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eChauhan A, Tikoo A, Kapur AK, Singh M. The taming of the cell penetrating domain of the HIV Tat: myths and realities. \u003cem\u003eJ Control Release.\u003c\/em\u003e 2007;117(2):148-62.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jconrel.2006.10.031\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813556154666,"sku":"BTS-B2026688","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2026688.png?v=1790897756","url":"https:\/\/bluetigerscientific.com\/products\/cys-tat-47-57-cell-penetrating-peptide","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}