{"product_id":"cm-agarose-bead","title":"CM Agarose Bead","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eCM Agarose Bead – Catalog #B2025394\u003c\/h2\u003e\n\u003cp\u003eCM Agarose Bead (Catalog #B2025394) is supplied as 25 mL of a cation exchange chromatography resin consisting of agarose beads with carboxymethyl (–CH₂–COO⁻) functional groups. The negatively charged carboxymethyl groups bind positively charged proteins (those with net positive charge at the working pH) while allowing negatively charged proteins and other molecules to flow through. The high porosity of the agarose matrix provides excellent flow properties, low back-pressure, and high protein binding capacity, making it suitable for both batch purification and gravity-flow column chromatography.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025394\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e25 mL (settled resin volume)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMatrix:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAgarose (6% crosslinked)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eFunctional group:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCarboxymethyl (–CH₂–COO⁻)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eChromatography mode:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCation exchange (weak anion exchanger at pH 4–9)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSettled resin suspension in storage buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCapture and purification of basic (pI \u0026gt;7) proteins, enzymes, antibodies, and recombinant proteins; preparative protein fractionation; batch chromatography and gravity-flow column separations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eRoom temperature (2–8°C preferred)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCM Agarose, carboxymethyl agarose, CM agarose resin, cation exchange resin, protein purification resin, agarose chromatography matrix, carboxymethylated agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eAgarose is a natural polymer derived from red algae and is widely used as a chromatography matrix because of its chemical stability, high porosity, low non-specific adsorption, and excellent compatibility with proteins and other biomolecules. Covalent attachment of carboxymethyl groups (–CH₂–COOH) to the agarose backbone creates a weakly acidic cation exchanger that functions over a broad pH range (typically pH 4–9). The ionizable carboxyl groups are negatively charged at neutral pH, binding proteins with a net positive charge (high pI, basic proteins) while allowing neutral and negatively charged molecules to pass through or be eluted at lower salt concentrations.\u003c\/p\u003e\n\u003cp\u003eThe agarose matrix's high porosity (large pore size) allows efficient diffusion of large molecules, including intact antibodies, enzymes, and multi-subunit complexes, into and out of the resin. This, combined with the high flow rates and low back-pressure typical of agarose chromatography, makes CM Agarose ideal for both analytical purifications and preparative-scale protein separations.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eCapture and concentration of basic enzymes and antibodies from crude extracts or fermentation broths\u003c\/li\u003e\n\u003cli\u003ePurification of recombinant proteins expressed in bacteria or eukaryotic cells\u003c\/li\u003e\n\u003cli\u003eSeparation of protein isozymes and variants based on pI differences\u003c\/li\u003e\n\u003cli\u003eBatch or gravity-flow chromatography for quick, efficient protein purifications\u003c\/li\u003e\n\u003cli\u003eRemoval of contaminants and impurities in protein purification workflows\u003c\/li\u003e\n\u003cli\u003eLaboratory-scale and preparative protein fractionation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eBefore use, allow the resin to settle and remove excess storage buffer by decanting or using a peristaltic pump. For batch chromatography, mix the resin slurry with your protein sample and allow binding to occur, then separate the resin from the flow-through by centrifugation or gravity settling. For column chromatography, pack the resin into a Ecad-style glass column or plastic chromatography column under gravity flow, then load the protein sample and elute with a salt gradient (e.g., 0–1 M NaCl) to recover bound proteins.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eEquilibration:\u003c\/strong\u003e Wash the resin with at least 10 column volumes of binding buffer (typically 20 mM Tris or phosphate, pH 7–8) before loading the sample.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBinding pH:\u003c\/strong\u003e The carboxymethyl groups are ionized at pH \u0026gt;4, so perform binding at pH 6–8 for optimal charge on both the resin and the target protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSalt gradient elution:\u003c\/strong\u003e Bind at low salt (~0 M NaCl) and elute with a linear gradient to 1 M NaCl or higher, depending on the protein's pI. Proteins with higher pI values will bind more strongly and require higher salt for elution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow rate:\u003c\/strong\u003e Agarose is pressure-tolerant and allows flow rates of 2–5 mL\/min for an 10 mm diameter column without excessive back-pressure. Faster flow rates can be used for initial sample loading.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegeneration and storage:\u003c\/strong\u003e After use, wash with 1 M NaCl, then water, then storage buffer (typically 20% ethanol in PBS). The resin can be stored at 2–8°C or room temperature in storage buffer for extended periods.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e25 mL of CM Agarose cation exchange resin\u003c\/li\u003e\n\u003cli\u003eA high-capacity, high-flow chromatography matrix for protein purification\u003c\/li\u003e\n\u003cli\u003eSuitable for batch and gravity-flow column applications\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eHigh porosity for efficient binding and elution of large proteins\u003c\/li\u003e\n\u003cli\u003eHigh flow rate with low back-pressure, ideal for gravity-flow columns\u003c\/li\u003e\n\u003cli\u003eExcellent protein binding capacity and selectivity\u003c\/li\u003e\n\u003cli\u003eCompatible with standard chromatography hardware\u003c\/li\u003e\n\u003cli\u003eCost-effective and robust for laboratory-scale purifications\u003c\/li\u003e\n\u003cli\u003eProven track record in enzyme and antibody purification\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhich proteins bind to CM Agarose?\u003c\/strong\u003e\u003cbr\u003eProteins with a net positive charge (pI \u0026gt;pH of the buffer) will bind. CM Agarose works best for basic proteins (pI \u0026gt;7) at neutral pH. Proteins with pI \u0026lt;7 will pass through and can be recovered in the flow-through.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I elute bound proteins?\u003c\/strong\u003e\u003cbr\u003eApply a linear salt gradient (e.g., 0–1 M NaCl) at the same pH used for binding. Proteins elute in order of increasing charge (lower pI first). Collect fractions and assay for your protein of interest.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat column size should I use?\u003c\/strong\u003e\u003cbr\u003eFor preparative work, use a column diameter such that the resin bed height is 5–20 cm. A 1 cm diameter column with 10 cm bed height contains ~7.9 mL of settled resin and is suitable for 5–50 mg of protein, depending on the target and contaminants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use this resin for FPLC or HPLC?\u003c\/strong\u003e\u003cbr\u003eCM Agarose is designed for gravity-flow and low-pressure chromatography. It can withstand moderate pressure, but for high-resolution, high-speed separations, use smaller-bead, higher-density ion exchange resins optimized for FPLC or HPLC.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I pack the column?\u003c\/strong\u003e\u003cbr\u003ePrepare a slurry of the resin in binding buffer, pour into a plastic or glass column, and allow to pack under gravity. Alternatively, gently apply low pressure (e.g., 0.5 bar) with a peristaltic pump to speed up packing. Avoid air bubbles; tap the column gently if needed to remove them.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long does the resin last?\u003c\/strong\u003e\u003cbr\u003eWith proper handling and regeneration, CM Agarose can be used for dozens of purifications over months or years. Store in 20% ethanol at 2–8°C or room temperature to prevent microbial growth.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a TDS or COA?\u003c\/strong\u003e\u003cbr\u003eYes. Request a quote or contact us and we will provide available lot documentation, binding capacity data, and pH range specifications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eAmeis D, Merkel M, Eckerskorn C, Greten H. Purification, characterization and molecular cloning of human hepatic lysosomal acid lipase. \u003cem\u003eEur J Biochem.\u003c\/em\u003e 1994;219(3):905-14.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1111\/j.1432-1033.1994.tb18572.x\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eMaharjan P, Hearn MT, Jackson WR, De Silva K, Woonton BW. Development of a temperature-responsive agarose-based ion-exchange chromatographic resin. \u003cem\u003eJ Chromatogr A.\u003c\/em\u003e 2009;1216(50):8722-9.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.chroma.2009.04.037\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSauer DG, Mosor M, Frank AC, Weiß F, Christler A, Walch N, Jungbauer A, Dürauer A. A two-step process for capture and purification of human basic fibroblast growth factor from E. coli homogenate: Yield versus endotoxin clearance. \u003cem\u003eProtein Expr Purif.\u003c\/em\u003e 2019;153:70-82.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.pep.2018.08.009\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813555302698,"sku":"BTS-B2025394","price":1185.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025394.png?v=1790897709","url":"https:\/\/bluetigerscientific.com\/products\/cm-agarose-bead","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}