{"product_id":"chemerin-9-149-157","title":"Chemerin-9 (149–157)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eChemerin-9 (149–157) – Catalog #B2026704\u003c\/h2\u003e\n\u003cp\u003eChemerin-9 (Catalog #B2026704) is supplied as 1 mg of a lyophilized peptide fragment derived from full-length chemerin. The peptide corresponds to amino acids 149–157 of chemerin and is a potent, well-characterized agonist of the ChemR23 receptor (also known as CMKLR1, chemokine-like receptor 1). Chemerin-9 retains the full biological activity of the parent protein toward ChemR23 and is widely used in research on immune cell recruitment, inflammation resolution, and G protein-coupled receptor signaling.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2026704\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003ePeptide sequence:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAmino acids 149–157 of human chemerin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1063.17 Da (approximate)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSynthetic peptide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBiological target:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eChemR23 (CMKLR1) G protein-coupled receptor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eImmune cell recruitment and activation studies, ChemR23 receptor signaling research, inflammation resolution, macrophage and dendritic cell biology, GPCR research\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e–20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eChemerin-9, Chemerin 149–157, chemerin peptide, ChemR23 agonist, CMKLR1 ligand, chemokine-like protein 1, inflammation peptide, immune cell recruitment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eChemerin is a 143–amino-acid protein belonging to the C19 family of serine protease substrate proteins. It circulates as an inactive prochemerin and is activated in vivo and in vitro by serine proteases (including coagulation proteases, complement proteases, and leukocyte-derived proteases) that cleave the C-terminal region. Full-length chemerin and its proteolytic fragments bind to the G protein-coupled receptor ChemR23 (CMKLR1), which is expressed on immature dendritic cells, tissue macrophages, and non-classical (intermediate and patrolling) monocytes. The chemerin–ChemR23 axis plays a key role in immune homeostasis, promoting the recruitment and anti-inflammatory polarization of immune cells during inflammation resolution.\u003c\/p\u003e\n\u003cp\u003eChemerin-9, the 149–157 fragment, is the core bioactive domain of full-length chemerin. It is a potent agonist of ChemR23 and is substantially more stable than full-length chemerin, which is prone to further proteolytic processing in cellular assays. Because of its compact size, low molecular weight, and superior in vitro stability, Chemerin-9 has become the standard tool for studying ChemR23-mediated immune responses in cell culture and in vivo models.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eInduction of immune cell chemotaxis toward ChemR23-expressing dendritic cells and macrophages\u003c\/li\u003e\n\u003cli\u003eEnhancement of phagocytosis in macrophages and dendritic cells via ChemR23 signaling\u003c\/li\u003e\n\u003cli\u003eMechanistic studies of inflammation resolution pathways\u003c\/li\u003e\n\u003cli\u003eChemR23 receptor antagonist screening and agonist characterization\u003c\/li\u003e\n\u003cli\u003eG protein-coupled receptor (GPCR) and G protein research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eDissolve the lyophilized peptide in sterile, ultrapure water or your assay buffer (phosphate-buffered saline, RPMI, or DMEM are all suitable). The peptide will readily dissolve; vortex or gently heat to speed dissolution if needed. Prepare a stock solution (typically 10 µM to 100 µM in PBS or assay medium), then aliquot and store frozen at –20°C or below to maintain stability. For cell culture assays, use stock solutions directly or dilute further into assay medium. For dose–response studies, titrate the peptide concentration from 0.1 nM to 1 µM depending on the assay and cell type.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eStock solution preparation:\u003c\/strong\u003e Dissolve in ultrapure water or PBS to a known concentration (e.g., 100 µM). Store aliquots at –20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell culture concentration:\u003c\/strong\u003e Typical working concentrations range from 1–100 nM in cell-based assays. Optimize for your specific cell type and assay endpoint (e.g., chemotaxis, phagocytosis, cytokine production).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e The lyophilized powder is stable for at least 1–2 years when stored at –20°C. Reconstituted solutions are stable for several days at 2–8°C and for weeks at –20°C when stored with 0.02% sodium azide as a preservative.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSterile handling:\u003c\/strong\u003e For cell culture work, use aseptic technique and filter sterilize stock solutions (0.22 µm) if contamination is a concern.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 mg of lyophilized Chemerin-9 (149–157) peptide\u003c\/li\u003e\n\u003cli\u003eHigh purity, suitable for cell culture and biochemical assays\u003c\/li\u003e\n\u003cli\u003eA potent, well-characterized ChemR23 agonist for immune cell research\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003ePotent ChemR23 receptor agonist with full biological activity\u003c\/li\u003e\n\u003cli\u003eMore stable than full-length chemerin in cell culture\u003c\/li\u003e\n\u003cli\u003eCompact, synthetic peptide suitable for standardized experiments\u003c\/li\u003e\n\u003cli\u003eWell-characterized in the immunology and GPCR literature\u003c\/li\u003e\n\u003cli\u003eIdeal for dose–response and mechanistic studies of immune signaling\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat cells express ChemR23?\u003c\/strong\u003e\u003cbr\u003eChemR23 is expressed on immature dendritic cells, tissue-resident macrophages, and non-classical (intermediate and patrolling) monocytes. Expression can also be induced on other cell types under certain inflammatory conditions. Use flow cytometry to confirm expression in your cell type of interest.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow does Chemerin-9 compare to full-length chemerin?\u003c\/strong\u003e\u003cbr\u003eChemerin-9 is the core bioactive domain of full-length chemerin and is a potent ChemR23 agonist. Unlike full-length chemerin, which can be further cleaved by proteases in cell culture medium, Chemerin-9 is resistant to proteolytic degradation, making it ideal for standardized cell assays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat assays can I use Chemerin-9 in?\u003c\/strong\u003e\u003cbr\u003eChemerin-9 is well-suited for migration assays (Boyden chamber, real-time impedance), phagocytosis assays, cytokine production assays (ELISA, Luminex), flow cytometry-based activation marker detection, and binding assays on ChemR23-expressing cells or receptor-transfected cell lines.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is a typical working concentration?\u003c\/strong\u003e\u003cbr\u003eMost cell-based assays use Chemerin-9 in the range of 1–100 nM. Optimal concentration depends on your cell type, assay format, and endpoint. Start with 10 nM and titrate up or down as needed.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use Chemerin-9 in vivo?\u003c\/strong\u003e\u003cbr\u003eYes, but because Chemerin-9 is a synthetic peptide, its pharmacokinetics in vivo may differ from full-length chemerin. Proteolytic stability in blood and tissue may also vary. For in vivo work, consult the literature for your specific organism and consider peptide-stabilizing modifications (e.g., PEGylation, D-amino acids).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I store the reconstituted peptide?\u003c\/strong\u003e\u003cbr\u003eStore at –20°C or below. To preserve sterility, add 0.02% sodium azide. Once reconstituted, the peptide is stable for at least 1–2 weeks at 2–8°C and several months at –20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a COA or TDS?\u003c\/strong\u003e\u003cbr\u003eYes. Request a quote or contact us and we will provide available lot documentation and information on peptide purity, mass spectrometry confirmation, and batch-specific storage guidance.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eCash JL, Christian AR, Greaves DR. Chemerin peptides promote phagocytosis in a ChemR23- and Syk-dependent manner. \u003cem\u003eJ Immunol.\u003c\/em\u003e 2010;184(9):5315-24.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.4049\/jimmunol.0903378\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLuangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M. Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model. \u003cem\u003eJ Immunol.\u003c\/em\u003e 2009;183(10):6489-99.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.4049\/jimmunol.0901037\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eZhang X, Weiß T, Cheng MH, Chen S, Ambrosius CK, Czerniak AS, Li K, Feng M, Bahar I, Beck-Sickinger AG, Zhang C. Structural basis of G protein-Coupled receptor CMKLR1 activation and signaling induced by a chemerin-derived agonist. \u003cem\u003ePLoS Biol.\u003c\/em\u003e 2023;21(12):e3002188.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1371\/journal.pbio.3002188\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eShimamura K, Matsuda M, Miyamoto Y, Yoshimoto R, Seo T, Tokita S. Identification of a stable chemerin analog with potent activity toward ChemR23. \u003cem\u003ePeptides.\u003c\/em\u003e 2009;30(8):1529-38.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.peptides.2009.05.030\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813556482346,"sku":"BTS-B2026704","price":465.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2026704.png?v=1790897773","url":"https:\/\/bluetigerscientific.com\/products\/chemerin-9-149-157","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}