{"product_id":"carboxylated-polystyrene-latex-beads-8-0-8-9-um","title":"Carboxylated Polystyrene Latex Beads (8.0–8.9 µm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eCarboxylated Polystyrene Latex Beads (8.0–8.9 µm) – Catalog #B2025466\u003c\/h2\u003e\n\u003cp\u003eCarboxylated polystyrene latex beads (Catalog #B2025466) are supplied as 5 mL of a uniform suspension of polymer spheres, 8.0 to 8.9 micrometers in diameter. Each particle carries carboxyl (–COOH) groups on its surface that are ionized at physiological pH, creating a stable negative charge. This carboxylation enables straightforward chemical coupling of proteins, peptides, antibodies, and other ligands via carbodiimide or other standard chemistries. The beads' larger micrometer size makes them easy to handle in routine laboratory workflows and suitable for applications where optical resolution or bead recovery is important.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025466\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSize range:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e8.0–8.9 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMaterial:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePolystyrene latex\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface charge:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eNegative (carboxylated)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAqueous suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eImmunoassays, bead-based diagnostics, flow cytometry and cell sorting, antibody immobilization, protein capture and purification, solid-phase assay platforms\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCarboxylated polystyrene beads, carboxyl latex microspheres, polystyrene microbeads, large-diameter beads, solid-phase beads, flow cytometry beads, antibody-coated beads, protein-binding microspheres\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003ePolystyrene has been the material of choice for bead-based diagnostics and immunoassays for decades because of its chemical stability, uniform synthesis at defined sizes, straightforward chemical modification, and reliable performance in automated and manual laboratory workflows. Carboxylation via copolymerization with methacrylic acid or post-synthetic treatment adds ionizable surface groups that are negatively charged at physiological pH. This negative charge stabilizes the suspension against aggregation (through electrostatic repulsion) and provides a chemical handle for ligand attachment via carbodiimide-mediated coupling, streptavidin–biotin interactions, or other conjugation strategies.\u003c\/p\u003e\n\u003cp\u003eAt 8–9 µm, these beads are at the larger end of the practical range for immunoassays and flow cytometry. At this size, beads are easy to see under a light microscope, can be recovered magnetically if paramagnetic material is incorporated, and provide sufficient surface area for high-affinity antibody or protein coating. The size is ideal for applications where the operator needs clear visual feedback, such as manual bead-based assays, and for flow-cytometry experiments where larger particles are preferred for optical resolution or gating.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eImmunoassays with high optical readout (bead-based ELISA, sandwich assays)\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and fluorescence-activated cell sorting (FACS) with antibody-coated beads\u003c\/li\u003e\n\u003cli\u003eManual bead-based assays where visual handling is important\u003c\/li\u003e\n\u003cli\u003eProtein capture and affinity purification\u003c\/li\u003e\n\u003cli\u003eBead-based diagnostic platforms and point-of-care assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the suspension at 2–8°C in the original container. Invert several times before use to ensure even distribution. For protein coating, dilute the beads into ultrapure water or phosphate-buffered saline and activate with carbodiimide (e.g., 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, or EDAC) and N-hydroxysuccinimide (NHS) to form reactive esters on the carboxyl groups, then incubate with the target protein or antibody. Specific coupling protocols depend on the protein's pI, concentration, and desired coating density; optimization may be required for your application.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivation:\u003c\/strong\u003e Use EDAC\/NHS chemistry to convert carboxyl groups to reactive N-hydroxysuccinimide esters for amine-coupling. Follow published protocols for your target biomolecule.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein concentration:\u003c\/strong\u003e For antibody coating, test a range of antibody concentrations (typically 1–10 µg\/mL) and incubation times to find the optimal density for your assay.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing and blocking:\u003c\/strong\u003e After coupling, wash beads thoroughly and block with BSA or non-fat milk to reduce non-specific binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVisual inspection:\u003c\/strong\u003e The larger bead size allows visual inspection for aggregation; beads should appear as a uniform suspension when resuspended.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of carboxylated polystyrene latex bead suspension\u003c\/li\u003e\n\u003cli\u003eUniform 8.0–8.9 µm particles with well-defined negative surface charge\u003c\/li\u003e\n\u003cli\u003eA convenient starting material for flow cytometry, immunoassay development, and bead-based diagnostic platforms\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eLarge, uniform size for easy visual handling and microscopy\u003c\/li\u003e\n\u003cli\u003eStraightforward surface chemistry for protein and antibody immobilization\u003c\/li\u003e\n\u003cli\u003eExcellent for flow cytometry and optical detection methods\u003c\/li\u003e\n\u003cli\u003eProven track record in bead-based immunoassays and diagnostics\u003c\/li\u003e\n\u003cli\u003eStable aqueous suspension ready to use\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I couple antibodies to the beads?\u003c\/strong\u003e\u003cbr\u003eActivate the carboxyl groups using EDAC\/NHS chemistry to form reactive succinimide esters, then incubate with your antibody of interest. The amine groups on your antibody will form amide bonds with the activated carboxyls. For optimal coating, test a range of antibody concentrations and incubation times specific to your antibody.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre these beads suitable for flow cytometry?\u003c\/strong\u003e\u003cbr\u003eYes, excellent. The 8–9 µm size is ideal for flow cytometry and allows for easy gating and optical resolution. Fluorescently labeled beads are commonly used as controls and in bead-based assays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I see the beads under a microscope?\u003c\/strong\u003e\u003cbr\u003eYes. At 8–9 µm, the beads are easily visible under a light microscope (40x or 100x objective), which makes manual assays and visual inspection straightforward.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the binding capacity?\u003c\/strong\u003e\u003cbr\u003eBinding capacity depends on the target protein's size and the desired coating density. Test with your specific antibody or protein to determine optimal coverage for your assay.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow should I store coated beads?\u003c\/strong\u003e\u003cbr\u003eStore coated beads at 2–8°C in storage buffer (typically PBS with 0.02% sodium azide as a preservative). Do not freeze unless specifically indicated.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I get a TDS or COA?\u003c\/strong\u003e\u003cbr\u003eYes. Request a quote or contact us and we will provide available lot documentation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGupta SK, Guesdon JL, Avrameas S, Talwar GP. Sandwich enzyme immunoassay of human chorionic gonadotropin using polystyrene beads as solid support. \u003cem\u003eAnn Inst Pasteur Immunol (1985).\u003c\/em\u003e 1985;136D(1):47-55.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0769-2625(85)80074-x\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eHashida S, Ishikawa S, Nakamoto H, Tanaka S, Kojima M, Ishikawa E. Simple and more sensitive immune complex transfer enzyme immunoassay for antibody IgG to reverse transcriptase of HIV-1 using microplates, modified polystyrene solid phase and fluororeader. \u003cem\u003eJ Clin Lab Anal.\u003c\/em\u003e 1996;10(5):294-301.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1002\/(SICI)1098-2825(1996)10:5\u0026lt;294::AID-JCLA10\u0026gt;3.0.CO;2-S\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eSchoof E, John MR, Arndt B, Gao P, Theuer D, Sieg A, Schmidt-Gayk H. Solid phase competitive luminescence immunoassay for immunoglobulin A in faeces: development and clinical validation. \u003cem\u003eClin Chim Acta.\u003c\/em\u003e 1997;261(1):1-17.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0009-8981(96)06510-2\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52813555106090,"sku":"BTS-B2025466","price":1195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025466.png?v=1790897700","url":"https:\/\/bluetigerscientific.com\/products\/carboxylated-polystyrene-latex-beads-8-0-8-9-um","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}