{"product_id":"carboxylate-modified-polystyrene-latex-beads-greater-than-10-um","title":"Carboxylate-Modified Polystyrene Latex Beads, \u003e10 µm","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eCarboxylate-Modified Polystyrene Latex Beads, \u0026gt;10 µm – Catalog #B202646\u003c\/h2\u003e\n\u003cp\u003eCarboxylate-Modified Polystyrene Latex Beads (Catalog #B202646) are large, monodisperse polystyrene microspheres functionalized with surface carboxyl groups. At \u0026gt;10 µm diameter in a 5 mL suspension, they are the largest in the carboxylate-modified polystyrene family, offering maximum ease of handling, visibility, and manual manipulation. The large particle size is ideal for applications where the assay workflow prioritizes convenience and visual inspection over maximum surface area: manual immunoassays, single-well or tube-based tests, magnetic bead separation, and educational demonstrations. Carboxyl groups are readily activated by EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) for straightforward protein coupling.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB202646\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead diameter:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e\u0026gt;10 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eComposition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePolystyrene with carboxylate surface groups\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eManual immunoassays, immunoprecipitation, cell sorting, magnetic separations, single-well assays, bead-based workflows, educational applications, large-scale affinity capture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLarge polystyrene beads, carboxylate-modified latex, \u0026gt;10 micron beads, visible microspheres, large-diameter particles, manual immunoassay beads, protein coupling beads, affinity capture beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eCarboxylate-modified polystyrene latex beads larger than 10 µm in diameter are used in applications where the bead-to-solution ratio, visibility, and ease of physical manipulation take priority over maximum surface area per unit volume. Even though larger beads have proportionally less surface area (a 15 µm sphere has only one-third the surface-area-to-volume ratio of a 5 µm sphere), a single large bead can still accommodate 100–500 protein molecules, sufficient for many detection and separation workflows.\u003c\/p\u003e\n\u003cp\u003eThe primary advantages of large beads include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eIndividual beads are easily visible to the naked eye and under standard light microscopy\u003c\/li\u003e\n\u003cli\u003eBeads settle rapidly under gravity, simplifying particle recovery without centrifugation\u003c\/li\u003e\n\u003cli\u003eEasy resuspension with gentle pipetting; no vortexing required\u003c\/li\u003e\n\u003cli\u003eCompatible with manual one-tube workflows and simple gravity-based separation\u003c\/li\u003e\n\u003cli\u003eIdeal substrate for magnetic core coating (creating large magnetic beads for high-throughput separators)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eManual immunoassays and affinity capture experiments\u003c\/li\u003e\n\u003cli\u003eImmunoprecipitation and co-IP from cell lysates or tissue homogenates\u003c\/li\u003e\n\u003cli\u003eBead-based cell sorting and enrichment\u003c\/li\u003e\n\u003cli\u003eEducational laboratory experiments and training\u003c\/li\u003e\n\u003cli\u003eCustom assay development where beads must be easily observed and manipulated by hand\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C. The large size ensures easy, trouble-free resuspension: simply invert the tube 3–5 times to mix. No vortexing or sonication is necessary. Beads will settle rapidly (within minutes) in aqueous solutions.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling chemistry:\u003c\/strong\u003e Activate carboxyl groups with EDC (10–40 mM in pH 5–6 coupling buffer) for 15–30 minutes. Add protein (10–100 µg\/mL) and incubate 1–4 hours at 20°C or overnight at 4°C with gentle inversion.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecovery by gravity:\u003c\/strong\u003e After incubation, allow beads to settle for a few minutes, then carefully remove the supernatant. No centrifugation is needed.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Resuspend in wash buffer (PBS + 0.1% Tween-20 or equivalent), allow to settle, and remove supernatant. Repeat 2–3 times as needed.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking:\u003c\/strong\u003e To minimize non-specific binding, incubate coupled beads with BSA (1–5% in PBS) for 30 minutes, then wash.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eUse in assays:\u003c\/strong\u003e Add to assay wells or tubes; beads settle naturally and are easy to track visually. Centrifugation is optional; gravity settling alone usually suffices.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of carboxylate-modified polystyrene latex beads at \u0026gt;10 µm diameter\u003c\/li\u003e\n\u003cli\u003eSupplied as ready-to-use suspension\u003c\/li\u003e\n\u003cli\u003eReactive carboxyl groups suitable for EDC\/NHS conjugation\u003c\/li\u003e\n\u003cli\u003eLarge size for maximum ease of handling and visualization\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eBeads are individually visible to the naked eye and easily tracked in assays\u003c\/li\u003e\n\u003cli\u003eSettle rapidly under gravity, eliminating need for centrifugation in many workflows\u003c\/li\u003e\n\u003cli\u003eExtremely easy to resuspend; no risk of aggregation or clumping with gentle inversion\u003c\/li\u003e\n\u003cli\u003eIdeal for manual, low-throughput assays and educational lab exercises\u003c\/li\u003e\n\u003cli\u003eProven chemistry: the same reactive carboxyl surface as smaller variants, just at a more convenient size for hands-on work\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much protein loads onto these large beads?\u003c\/strong\u003e\u003cbr\u003eA 15 µm bead has a surface area of roughly 700 µm² and can typically accommodate 100–500 protein molecules, depending on protein size and coupling efficiency. This is sufficient for most immunoassay and affinity-capture workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDo I need to centrifuge to recover the beads?\u003c\/strong\u003e\u003cbr\u003eNo. Large beads settle rapidly (within 5–10 minutes) under gravity, so you can simply wait and carefully pipette off the supernatant. Centrifugation is optional and not necessary for most applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these in flow cytometry?\u003c\/strong\u003e\u003cbr\u003eStandard flow cytometers are typically optimized for beads in the 1–10 µm range. These very large beads may not pass through narrow nozzles or may clog instruments. For flow cytometry, choose smaller variants (1.0–2.9 µm).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre these beads suitable for high-throughput screening?\u003c\/strong\u003e\u003cbr\u003eThese beads are best suited for manual, low-to-moderate-throughput workflows. For automated high-throughput screening, choose smaller beads (1–3 µm) that are compatible with automated plate readers and liquid handlers.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I make magnetic beads from these?\u003c\/strong\u003e\u003cbr\u003eYes. Larger beads can be coated with or attached to magnetic particles to create large magnetic beads, which are highly convenient for automated magnetic bead handlers and separators.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do the prices compare for different sizes?\u003c\/strong\u003e\u003cbr\u003ePrice variation reflects manufacturing and handling differences. Larger beads are often less costly per particle but provide less total surface area. Request a quote to compare by cost per assay or per square meter of surface area.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eMolina-Bolívar JA, Galisteo-González F, Hidalgo-Alvarez R. Particle enhanced immunoassays stabilized by hydration forces: a comparative study between IgG and F(ab)2 immunoreactivity. \u003cem\u003eJ Immunol Methods.\u003c\/em\u003e 1998;211(1-2):87-95.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0022-1759(97)00189-0\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eFortin M, Hugo P. Surface antigen detection with non-fluorescent, antibody-coated microbeads: an alternative method compatible with conventional fluorochrome-based labeling. \u003cem\u003eCytometry.\u003c\/em\u003e 1999;36(1):27-35.\u003c\/span\u003e\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/10331624\/\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKamyshny A, Feldman A, Baszkin A, Boissonnade MM, Rosilio V V, Magdassi S. Chemically Modified Glucose Oxidase with Enhanced Hydrophobicity: Adsorption at Polystyrene, Silica, and Silica Coated by Lipid Monolayers. \u003cem\u003eJ Colloid Interface Sci.\u003c\/em\u003e 1999;218(1):300-308.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1006\/jcis.1999.6440\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52809024209194,"sku":"BTS-B202646","price":705.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B202646.png?v=1790859906","url":"https:\/\/bluetigerscientific.com\/products\/carboxylate-modified-polystyrene-latex-beads-greater-than-10-um","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}