{"product_id":"carboxylate-modified-polystyrene-latex-beads-greater-than-10-um-2-5-w-v","title":"Carboxylate-Modified Polystyrene Latex Beads, \u003e10 µm, 2.5% w\/v","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eCarboxylate-Modified Polystyrene Latex Beads, \u0026gt;10 µm, 2.5% w\/v – Catalog #B2025720\u003c\/h2\u003e\n\u003cp\u003eCarboxylate-Modified Polystyrene Latex Beads (Catalog #B2025720) are large, monodisperse polystyrene microspheres functionalized with surface carboxyl groups. At \u0026gt;10 µm diameter supplied at 2.5% w\/v in a 5 mL suspension, they combine the practical advantages of large particle size—easy visibility, rapid gravity settling, and convenient manual handling—with a higher bead concentration for applications requiring abundant particles. The large size makes these beads ideal for immunoprecipitation, cell sorting, manual sandwich assays, and any workflow where the researcher needs to observe and manipulate individual beads. Carboxyl groups are readily activated by EDC\/carbodiimide chemistry for covalent protein coupling.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025720\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead diameter:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e\u0026gt;10 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2.5% w\/v\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eComposition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePolystyrene with carboxylate surface groups\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eImmunoprecipitation, manual immunoassays, cell sorting, affinity capture, bead-based separation, single-tube workflows, magnetic bead preparation, educational applications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eLarge polystyrene beads, carboxylate-modified latex, \u0026gt;10 micron beads, visible microspheres, 2.5% suspension, manual immunoassay beads, protein coupling beads, high-concentration bead suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eCarboxylate-modified polystyrene latex beads greater than 10 µm in diameter are among the largest commercially available functional microbeads. Although their surface-area-to-volume ratio is lower than smaller variants (a 15 µm bead has one-third the surface area per unit volume of a 5 µm bead), the large size offers compelling practical advantages: individual beads are visible to the naked eye and under standard light microscopy, particles settle rapidly under gravity without requiring centrifugation, and gentle inversion (not vortexing) is sufficient for resuspension.\u003c\/p\u003e\n\u003cp\u003eSupplied at 2.5% w\/v, this variant offers a higher particle concentration than standard lower-concentration suspensions, making it ideal for applications where many beads are needed per reaction or where rapid particle settling is advantageous. The carboxyl surface groups are readily activated by EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide), enabling straightforward covalent coupling of proteins, antibodies, peptides, and other amino-containing molecules via standard carbodiimide chemistry.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eImmunoprecipitation and co-immunoprecipitation (co-IP) from cell lysates or tissue extracts\u003c\/li\u003e\n\u003cli\u003eManual, tube-based immunoassays and affinity-capture workflows\u003c\/li\u003e\n\u003cli\u003eSingle-bead or limited-bead-number applications where bead visibility is essential\u003c\/li\u003e\n\u003cli\u003eBead-based cell sorting and enrichment from complex samples\u003c\/li\u003e\n\u003cli\u003eSubstrate for creating large magnetic beads when coated with magnetic particles\u003c\/li\u003e\n\u003cli\u003eEducational demonstrations and student laboratory experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore at 2–8°C and protect from excessive agitation. Before use, gently invert the tube 3–5 times to evenly distribute beads. The large particle size and high concentration ensure reliable, bubble-free resuspension without vortexing or sonication.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling procedure:\u003c\/strong\u003e Activate beads with EDC (10–40 mM in pH 5–6 coupling buffer) for 15–30 minutes with gentle inversion. Add protein (10–100 µg\/mL) and incubate 1–4 hours at room temperature or overnight at 4°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBead recovery by gravity:\u003c\/strong\u003e After incubation, allow beads to settle for 5–10 minutes, then carefully remove supernatant by pipetting. No centrifugation is required.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Resuspend settled beads in wash buffer (PBS + 0.1% Tween-20 or equivalent), allow to settle, and remove supernatant. Repeat 2–3 times.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein loading estimation:\u003c\/strong\u003e Large beads at \u0026gt;10 µm typically accommodate 100–500 protein molecules depending on protein size and coupling efficiency. This is sufficient for most affinity-capture and immunoprecipitation workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBlocking non-specific binding:\u003c\/strong\u003e Incubate coupled beads with BSA (2–5% in PBS) for 30 minutes with gentle mixing, then wash, to block the hydrophobic polystyrene surface.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of carboxylate-modified polystyrene latex beads at \u0026gt;10 µm diameter\u003c\/li\u003e\n\u003cli\u003eSupplied at 2.5% w\/v concentration for high particle density\u003c\/li\u003e\n\u003cli\u003eReactive carboxyl groups suitable for EDC\/NHS conjugation\u003c\/li\u003e\n\u003cli\u003eLarge size for maximum ease of handling and visualization\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eLarge beads are individually visible and easily tracked throughout assays\u003c\/li\u003e\n\u003cli\u003eRapid gravity settling (5–10 minutes) eliminates need for centrifugation in most workflows\u003c\/li\u003e\n\u003cli\u003eHigher concentration (2.5% w\/v) provides abundant particles for high-bead-number applications\u003c\/li\u003e\n\u003cli\u003eGentle resuspension (inversion only); no risk of aggregation or damage from vortexing\u003c\/li\u003e\n\u003cli\u003eProven chemistry and reliability for manual, low-to-moderate-throughput assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between this product (B2025720) and B202646?\u003c\/strong\u003e\u003cbr\u003eBoth are large (\u0026gt;10 µm) beads with the same carboxyl-modified polystyrene chemistry. B2025720 is supplied at 2.5% w\/v (higher bead concentration), while B202646 is supplied at a lower standard concentration. Choose B2025720 if you need more beads per unit volume.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow many beads per milliliter at 2.5% w\/v?\u003c\/strong\u003e\u003cbr\u003eA 2.5% w\/v suspension of large (15 µm) beads contains roughly 10^10 to 10^11 particles per mL, depending on the exact bead size distribution. Request a COA for lot-specific particle concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I couple multiple proteins sequentially to the same beads?\u003c\/strong\u003e\u003cbr\u003eTheoretically yes, but practically not recommended. The large surface area of one large bead is typically saturated by a single protein species. For multiplex assays, use smaller beads or separate beads for each protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre these beads suitable for automated assay platforms?\u003c\/strong\u003e\u003cbr\u003eThese large beads are best suited for manual, low-throughput workflows. For automated high-throughput screening (e.g., plate readers, liquid handlers), use smaller beads (1–3 µm) designed for microplate compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long do protein-coupled beads remain stable?\u003c\/strong\u003e\u003cbr\u003eProtein-coupled beads are stable for several months at 2–8°C, or longer at −20°C in a cryoprotectant such as 50% glycerol. Include an antimicrobial preservative (0.02% sodium azide or Proclin) to prevent microbial contamination during extended storage.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat dilution should I use for assays?\u003c\/strong\u003e\u003cbr\u003eStart with the stock 2.5% w\/v suspension and dilute as needed (typically 1:10 to 1:100 depending on the assay). Dilution in PBS or assay buffer maintains bead stability and prevents clumping.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads for magnetic separation?\u003c\/strong\u003e\u003cbr\u003eThese are non-magnetic polystyrene beads. However, they can be coated with or attached to magnetic particles to create large magnetic beads, which are very convenient for automated magnetic separators.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eMolina-Bolívar JA, Galisteo-González F, Hidalgo-Alvarez R. Particle enhanced immunoassays stabilized by hydration forces: a comparative study between IgG and F(ab)2 immunoreactivity. \u003cem\u003eJ Immunol Methods.\u003c\/em\u003e 1998;211(1-2):87-95.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0022-1759(97)00189-0\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eFortin M, Hugo P. Surface antigen detection with non-fluorescent, antibody-coated microbeads: an alternative method compatible with conventional fluorochrome-based labeling. \u003cem\u003eCytometry.\u003c\/em\u003e 1999;36(1):27-35.\u003c\/span\u003e\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/10331624\/\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKamyshny A, Feldman A, Baszkin A, Boissonnade MM, Rosilio V V, Magdassi S. Chemically Modified Glucose Oxidase with Enhanced Hydrophobicity: Adsorption at Polystyrene, Silica, and Silica Coated by Lipid Monolayers. \u003cem\u003eJ Colloid Interface Sci.\u003c\/em\u003e 1999;218(1):300-308.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1006\/jcis.1999.6440\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52809023947050,"sku":"BTS-B2025720","price":705.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025720.png?v=1790859889","url":"https:\/\/bluetigerscientific.com\/products\/carboxylate-modified-polystyrene-latex-beads-greater-than-10-um-2-5-w-v","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}