{"product_id":"carboxylate-modified-polystyrene-latex-beads-0-6-1-0-um-2-5-w-v","title":"Carboxylate-Modified Polystyrene Latex Beads, 0.6–1.0 µm, 2.5% w\/v","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eCarboxylate-Modified Polystyrene Latex Beads, 0.6–1.0 µm, 2.5% w\/v – Catalog #B2025701\u003c\/h2\u003e\n\u003cp\u003eCarboxylate-Modified Polystyrene Latex Beads (Catalog #B2025701) are monodisperse polystyrene microspheres functionalized with carboxyl groups. Supplied at 0.6–1.0 µm diameter in a 5 mL suspension at 2.5% w\/v, they provide a high-surface-area substrate for covalent protein coupling, immunoassay development, and affinity-based biomolecule capture. The reactive carboxyl groups are readily activated for conjugation via EDC\/NHS or carbodiimide-mediated chemistry, allowing straightforward immobilization of antibodies, antigens, proteins, and peptides.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025701\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead diameter:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e0.6–1.0 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2.5% w\/v\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eComposition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ePolystyrene with carboxylate surface groups\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eProtein coupling, EDC\/NHS conjugation, immunoassays, affinity capture, flow cytometry, bioseparations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCarboxylate-modified polystyrene, carboxyl latex beads, EDC-activatable beads, microspheres, polymer beads, protein coupling beads, carboxyl-functionalized polystyrene, affinity beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 µm.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eCarboxylate-modified polystyrene latex beads are colloidal particles widely used in molecular diagnostics, affinity separations, and protein chemistry. The carboxyl (-COOH) functional groups at the particle surface enable covalent coupling of protein and peptide ligands via the EDC\/carbodiimide pathway, converting the carboxyl group to a reactive acyl intermediate that reacts with primary amines (lysine residues, N-termini) on the protein.\u003c\/p\u003e\n\u003cp\u003eAt 0.6–1.0 µm diameter, these beads offer an exceptionally high surface-area-to-volume ratio. For example, a 1 µm sphere has roughly 9-fold more surface area per unit volume than a 3 µm sphere, allowing for higher ligand density and amplification in assay systems. The small size also improves homogeneity in suspension and is well-suited for flow cytometry and high-resolution imaging.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eProtein and peptide immobilization for immunoassays, ELISA, and sandwich assays\u003c\/li\u003e\n\u003cli\u003eAffinity capture of proteins, antibodies, and biotinylated molecules\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and immunophenotyping platforms\u003c\/li\u003e\n\u003cli\u003eBioseparations and magnetic bead-based purification (when used with magnetic cores)\u003c\/li\u003e\n\u003cli\u003eDNA\/RNA capture when conjugated to complementary probes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the suspension at 2–8°C. Before use, gently mix (invert the tube several times) to ensure even distribution. Do not vortex or subject to ultrasound, as this may damage the beads or promote aggregation.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugation protocol:\u003c\/strong\u003e Activate carboxyl groups with EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) and optionally NHS (N-hydroxysuccinimide) to extend the lifetime of the activated intermediate. Add protein in appropriate coupling buffer (pH 4.5–7.5, depending on chemistry) and incubate for 1–4 hours at room temperature or overnight at 4°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBuffer choice:\u003c\/strong\u003e EDC coupling is most efficient at pH 5–6 but can tolerate pH 4.5–8.5. Higher pH favors activation but reduces protein stability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoupling density:\u003c\/strong\u003e The number of protein molecules per bead depends on protein size, activation level, and incubation time. Typical values range from 100–1000 copies per bead; request a COA for lot-specific coupling capacity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuenching and blocking:\u003c\/strong\u003e After coupling, quench unreacted carboxyl groups with ethanolamine, hydroxylamine, or excess Tris to prevent non-specific binding.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of carboxylate-modified polystyrene latex beads at 0.6–1.0 µm diameter\u003c\/li\u003e\n\u003cli\u003eSupplied at 2.5% w\/v suspension for immediate use\u003c\/li\u003e\n\u003cli\u003eReactive carboxyl groups ready for EDC\/carbodiimide conjugation\u003c\/li\u003e\n\u003cli\u003eHigh surface area per unit volume for sensitive assays\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eUniform, monodisperse size ensures predictable bead behavior and minimal batch-to-batch variation\u003c\/li\u003e\n\u003cli\u003eHigh surface-area-to-volume ratio maximizes ligand loading and assay sensitivity\u003c\/li\u003e\n\u003cli\u003eReactive carboxyl groups are straightforward to activate and couple with standard EDC\/NHS chemistry\u003c\/li\u003e\n\u003cli\u003eSmall size is ideal for flow cytometry, imaging, and suspension-based assays\u003c\/li\u003e\n\u003cli\u003eWell-characterized chemistry backed by decades of immunoassay and bead-based diagnostics literature\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I couple a protein to these beads?\u003c\/strong\u003e\u003cbr\u003eActivate carboxyl groups with EDC in coupling buffer (pH 5–6), add your protein (10–1000 µg\/mL), and incubate 1–4 hours at room temperature or overnight at 4°C. Quench with ethanolamine or Tris and wash to remove unbound protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the coupling capacity of these beads?\u003c\/strong\u003e\u003cbr\u003eCoupling density depends on protein size and activation level. Typical values are 100–1000 protein molecules per bead. Request a COA or datasheet for lot-specific information, or determine empirically by quantifying bound protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads in magnetic separation?\u003c\/strong\u003e\u003cbr\u003eThese beads are non-magnetic polystyrene. For magnetic separation, use carboxylate-modified magnetic beads or combine these with magnetic particles.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow long will the coupled protein remain stable?\u003c\/strong\u003e\u003cbr\u003eStability depends on storage conditions, buffer composition, and the protein itself. Generally, protein-coupled beads are stable for months at 2–8°C or longer at −20°C in a cryoprotectant (e.g., 50% glycerol).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I couple multiple different proteins to the same batch?\u003c\/strong\u003e\u003cbr\u003eYes. You can split the bead suspension and couple different proteins to separate portions, or sequentially couple proteins if their pH and buffer requirements are compatible.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhy use 0.6–1.0 µm beads instead of larger sizes?\u003c\/strong\u003e\u003cbr\u003eSmaller beads have greater surface area per unit volume, allowing higher ligand density and better assay sensitivity. They also flow smoothly in cytometers and imaging instruments and are ideal for detecting rare targets.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eMolina-Bolívar JA, Galisteo-González F, Hidalgo-Alvarez R. Particle enhanced immunoassays stabilized by hydration forces: a comparative study between IgG and F(ab)2 immunoreactivity. \u003cem\u003eJ Immunol Methods.\u003c\/em\u003e 1998;211(1-2):87-95.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/s0022-1759(97)00189-0\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eFortin M, Hugo P. Surface antigen detection with non-fluorescent, antibody-coated microbeads: an alternative method compatible with conventional fluorochrome-based labeling. \u003cem\u003eCytometry.\u003c\/em\u003e 1999;36(1):27-35.\u003c\/span\u003e\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/10331624\/\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKamyshny A, Feldman A, Baszkin A, Boissonnade MM, Rosilio V V, Magdassi S. Chemically Modified Glucose Oxidase with Enhanced Hydrophobicity: Adsorption at Polystyrene, Silica, and Silica Coated by Lipid Monolayers. \u003cem\u003eJ Colloid Interface Sci.\u003c\/em\u003e 1999;218(1):300-308.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1006\/jcis.1999.6440\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52809023914282,"sku":"BTS-B2025701","price":705.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025701.png?v=1790859883","url":"https:\/\/bluetigerscientific.com\/products\/carboxylate-modified-polystyrene-latex-beads-0-6-1-0-um-2-5-w-v","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}