{"product_id":"carboxyl-magnetic-beads-1-um","title":"Carboxyl Magnetic Beads (1 µm)","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eCarboxyl Magnetic Beads (1 µm) – Catalog #B2025725\u003c\/h2\u003e\n\u003cp\u003eThese 1 µm carboxyl-coated magnetic beads represent a significant advance in miniaturized affinity capture technology. The submicron size increases surface area-to-volume ratio by roughly 10-fold compared to 10 µm beads, accelerating both binding kinetics and magnetic separation. A ferromagnetic core ensures rapid sedimentation in magnetic fields, while the carboxyl surface enables covalent coupling of antibodies, biotin, streptavidin, and other targeting molecules via EDC or carbodiimide chemistry. The small size permits high particle counts per unit volume, enhancing capture capacity and enabling sensitive detection in diagnostic and research applications. Supplied as a stable liquid suspension at high particle concentration, they are ready to use or couple without additional processing.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog Number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025725\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eParticle Size:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e1 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead Material:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic polymer core (iron oxide particles embedded in polymeric matrix)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface Functionality:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCarboxyl groups (–COOH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied As:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAqueous suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eMagnetic separation, protein capture, immunomagnetic isolation, affinity purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ecarboxylated magnetic beads, carboxyl magnetic particles, carboxyl functionalized, carboxylic acid magnetic, magnetic beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eSubmicron magnetic beads represent a powerful tool for affinity-based separation and capture. At 1 µm diameter, these particles offer a surface area that is approximately 100-fold larger (per unit mass) than 10 µm beads. This increase translates to proportionally faster binding kinetics, as target molecules encounter more available binding sites per unit time. The magnetic core—a composite of iron oxide nanoparticles embedded in a polymeric matrix—provides superparamagnetic behavior: the beads develop a strong magnetic moment in external fields but show no residual magnetization when the field is removed, preventing irreversible clumping and enabling complete release of targets.\u003c\/p\u003e\n\u003cp\u003eCarboxyl groups on the bead surface are excellent platforms for EDC-mediated protein coupling. The chemistry is straightforward: EDC activates carboxyl groups to form reactive O-acylisourea intermediates, which react with primary amines (on lysines and N-terminal amines of proteins) to form stable amide bonds. Unlike biotin–streptavidin or other reversible capture systems, covalent coupling via carbodiimides creates permanent attachments suitable for reusable capture reagents and high-affinity immunoassays. The small bead size permits high particle concentration per unit volume, maximizing capture capacity and permitting sensitive detection in diagnostic platforms.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eImmunomagnetic separation of cells, pathogens, or target proteins from biological samples\u003c\/li\u003e\n\u003cli\u003eRapid affinity capture for sample preparation in mass spectrometry and proteomics workflows\u003c\/li\u003e\n\u003cli\u003eDiagnostic assays: antibody-coated beads capture biomarkers for quantitation and detection\u003c\/li\u003e\n\u003cli\u003eProtein purification: His-tagged or epitope-tagged protein capture from cell lysates or fermentation broths\u003c\/li\u003e\n\u003cli\u003eCirculating biomarker isolation: capture rare cells, exosomes, or DNA fragments from blood or tissue fluids\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eTo couple proteins, gently resuspend the bead suspension by pipetting (avoid vortexing, which may cause aggregation). Transfer the desired volume to a microcentrifuge tube and apply a strong magnetic field to sediment beads. Remove the storage buffer and resuspend beads in coupling buffer (10–25 mM MES, pH 5.5–6.5). Add EDC to 20–50 mM, incubate for 5 minutes, then add your target protein and incubate 30 minutes to 2 hours at room temperature. Wash by magnetic separation 3 times in PBS. Coupled beads may be stored at 2–8 °C in PBS with 0.1% BSA or used immediately in capture protocols.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eUse a strong neodymium magnet (neodymium-iron-boron, N52 or stronger) to ensure rapid sedimentation\u003c\/li\u003e\n\u003cli\u003eAlways include a protein carrier (0.1–1% BSA, casein, or non-fat milk) in storage and blocking buffers\u003c\/li\u003e\n\u003cli\u003eAvoid vortexing and excessive mixing; use gentle pipetting to resuspend to prevent bead aggregation\u003c\/li\u003e\n\u003cli\u003eFor blocking, pre-incubate coupled beads in PBS + 5–10% BSA or serum for 30 minutes to an hour\u003c\/li\u003e\n\u003cli\u003eInclude detergent (0.05–0.1% Triton X-100 or Tween-20) in capture and wash buffers to reduce non-specific binding\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e1 mL of 1 µm carboxyl magnetic bead suspension at high particle concentration\u003c\/li\u003e\n\u003cli\u003eBeads supplied in a storage buffer optimized for long-term stability\u003c\/li\u003e\n\u003cli\u003eBeads ready to couple with proteins via EDC chemistry or ready for direct use in capture applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eSubmicron size provides 10–100-fold higher surface area per unit mass than larger beads, accelerating binding\u003c\/li\u003e\n\u003cli\u003eSuperparamagnetic behavior enables complete reversibility and prevents irreversible aggregation\u003c\/li\u003e\n\u003cli\u003eHigh particle count per mL maximizes capture capacity and enables sensitive detection\u003c\/li\u003e\n\u003cli\u003eCarboxyl surface enables quick, stoichiometric coupling via standard EDC chemistry\u003c\/li\u003e\n\u003cli\u003eSmall size permits integration into microfluidic devices, automated high-throughput platforms, and diagnostic instruments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the maximum protein coupling density?\u003c\/strong\u003e\u003cbr\u003eAt 1 µm diameter, a single bead offers roughly 3 µm² of surface area. Carboxyl density is typically 100–1000 groups per µm². Coupling efficiency to amines ranges from 60–95%, yielding roughly 10⁶–10⁷ protein molecules per bead (depending on protein size and coupling conditions).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow quickly do these beads sediment in a magnetic field?\u003c\/strong\u003e\u003cbr\u003eAt 1 µm size with embedded magnetic cores, sedimentation is very rapid—typically 30 seconds to 1 minute with a strong (neodymium) magnet. Larger beads (10 µm) sediment in seconds; smaller beads (~100 nm) may take 2–5 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAre these beads suitable for microfluidic or lab-on-a-chip applications?\u003c\/strong\u003e\u003cbr\u003eYes. The 1 µm size is ideal for integration into microfluidic devices. However, ensure that device channel widths and flow rates are optimized to prevent bead clogging; typical minimum channel widths are 5–10 µm.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I use these beads in an automated liquid handler or pipetting robot?\u003c\/strong\u003e\u003cbr\u003eYes, but use wide-bore pipette tips (200 µL or larger) and avoid excessive shearing or vortexing. Configure the liquid handler to use gentle pipetting parameters to prevent aggregation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between 1 µm and 10 µm magnetic beads?\u003c\/strong\u003e\u003cbr\u003e1 µm beads offer 10-fold higher surface area per unit mass, faster kinetics, and higher particle concentration per mL. 10 µm beads sediment faster and are easier to handle manually. Choose 1 µm for kinetic speed and sensitivity; choose 10 µm for ease of manual manipulation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I freeze and thaw these beads?\u003c\/strong\u003e\u003cbr\u003eFreezing is not recommended for liquid suspensions, as ice crystal formation can damage the beads and cause aggregation. Store at 2–8 °C. If freezing is necessary, resuspend beads in a cryoprotective buffer containing 10–20% glycerol or ethylene glycol before freezing, and thaw slowly at 4 °C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1049\/iet-nbt.2019.0271\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1007\/s00216-016-9510-3\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1021\/acsabm.4c00280\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52809022570794,"sku":"BTS-B2025725","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025725.png?v=1790859795","url":"https:\/\/bluetigerscientific.com\/products\/carboxyl-magnetic-beads-1-um","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}