{"product_id":"carboxyl-magnetic-agarose-beads","title":"Carboxyl Magnetic Agarose Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eCarboxyl Magnetic Agarose Beads – Catalog #B2025449\u003c\/h2\u003e\n\u003cp\u003eCarboxyl magnetic agarose beads combine the superior protein-binding properties of agarose with embedded magnetic iron oxide cores and reactive carboxyl surface groups. Agarose is a naturally derived, inert polysaccharide that has been the gold standard for protein purification and affinity chromatography for decades. The macroporous structure minimizes viscous drag, allows rapid diffusion of proteins, and provides low non-specific binding compared to synthetic polymers. The carboxyl surface groups enable direct coupling of antibodies, antigens, enzymes, and other proteins via EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) or related carbodiimide chemistry. The integrated magnetic core permits rapid, reversible separation using a simple handheld magnet—eliminating the need for centrifugation, filtration, or gel-based purification.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog Number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025449\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBead Composition:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAgarose core with embedded magnetic particles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSurface Functionality:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eCarboxyl groups (–COOH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied As:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension in storage buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eProtein affinity capture, immunoprecipitation, co-immunoprecipitation, protein purification, magnetic separation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003ecarboxylated magnetic agarose beads, carboxyl magnetic beads, magnetic agarose beads, carboxyl-functionalized magnetic, affinity beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eAgarose is a linear polysaccharide extracted from marine red algae, composed of alternating galactose and 3,6-anhydrogalactose residues. Its neutral, uncharged structure creates a biocompatible matrix with minimal non-specific interactions with proteins. The gel's macroporous architecture (typical pore sizes 10–100 nm depending on agarose concentration) allows large protein complexes and antibodies to penetrate and bind efficiently. This property makes agarose the preferred material for native (non-denaturing) purification of protein complexes, membrane proteins, and multi-subunit assemblies.\u003c\/p\u003e\n\u003cp\u003eThe carboxyl surface coating provides reactive anchor points for covalent attachment of targeting ligands. Carboxyl groups are activated by EDC, which forms an unstable O-acylisourea intermediate. This intermediate reacts spontaneously with primary amines (lysine residues and N-terminal amines on proteins), creating a stable amide bond. This chemistry is biocompatible, rapid, and does not require organic solvents, making it the standard approach for attaching proteins and antibodies to magnetic beads. The magnetic cores embedded within the beads respond to external magnetic fields, permitting rapid sedimentation and separation of bead-bound targets from solution without centrifugation or filtration.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eCo-immunoprecipitation (co-IP): isolate protein complexes by pulling down with a primary antibody-bound capture bead\u003c\/li\u003e\n\u003cli\u003eAffinity purification: capture His-tagged, FLAG-tagged, or antigen-specific proteins from cell lysates or crude extracts\u003c\/li\u003e\n\u003cli\u003eAntibody purification: bind specific antigens to beads and use them to capture antibodies from serum or culture supernatant\u003c\/li\u003e\n\u003cli\u003eImmune cell enrichment: coat beads with antibodies against surface markers (CD4, CD8, HLA, etc.) and capture specific cell populations\u003c\/li\u003e\n\u003cli\u003eBiomarker isolation: capture disease-specific antigens or biomarkers from biological samples for downstream analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eTo couple proteins or antibodies, resuspend beads gently by pipetting, transfer to a microcentrifuge tube, and apply a magnetic field (magnet to the outside of the tube) to sedimented particles. Remove the storage buffer and resuspend beads in coupling buffer (typically 25 mM MES, pH 5.0–6.0). Add EDC to a final concentration of 10–50 mM and incubate beads for 5 minutes. Add your target protein and incubate 30 minutes to 2 hours at room temperature or 4 °C. Wash beads three times by magnetic separation in PBS. The coupled beads are now ready for capture experiments or storage at 2–8 °C in PBS with 0.1% BSA or casein to prevent aggregation.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eUse a strong neodymium or samarium-cobalt magnet for rapid sedimentation (30 seconds to 2 minutes per wash)\u003c\/li\u003e\n\u003cli\u003eAlways include a carrier protein (BSA, casein, or milk powder) in storage and blocking buffers to minimize non-specific aggregation\u003c\/li\u003e\n\u003cli\u003eOptimize EDC concentration (10–50 mM) and incubation time based on your specific protein; lower EDC and shorter incubation times reduce non-specific cross-linking\u003c\/li\u003e\n\u003cli\u003ePre-incubate coupled beads in blocking buffer (PBS + 5% BSA or goat serum) for 30 minutes before capture experiments\u003c\/li\u003e\n\u003cli\u003eFor removal of excess EDC and unreacted coupling reagents, perform 3–5 magnetic washes in PBS before storing or using\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of carboxyl magnetic agarose bead suspension\u003c\/li\u003e\n\u003cli\u003eBeads supplied in a stabilizing buffer with minimal aggregation\u003c\/li\u003e\n\u003cli\u003eProduct ready to couple with proteins via EDC chemistry or to use directly in capture protocols\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eAgarose matrix minimizes non-specific protein binding while supporting high coupling densities\u003c\/li\u003e\n\u003cli\u003eMacroporous structure allows efficient capture of large protein complexes and antibodies\u003c\/li\u003e\n\u003cli\u003eCarboxyl groups enable fast, mild, reproducible coupling via standard EDC chemistry\u003c\/li\u003e\n\u003cli\u003eMagnetic separation eliminates centrifugation, filtration, and column chromatography steps\u003c\/li\u003e\n\u003cli\u003eBiocompatible material is ideal for native, non-denaturing protein purification and interaction studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow much protein can I couple to each bead?\u003c\/strong\u003e\u003cbr\u003eCoupling capacity depends on carboxyl group density, protein size, and EDC concentration. Typical literature values range from 50–200 µg of protein per mL of beads, equivalent to roughly 10⁶–10⁷ protein molecules per bead (for a typical 10 µm bead). Empirical optimization with your protein is recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat EDC concentration should I use?\u003c\/strong\u003e\u003cbr\u003eStart with 20–30 mM EDC for 30 minutes at room temperature. Higher EDC (50 mM) or longer incubation (2 hours) increases coupling but also increases non-specific cross-linking and bead aggregation. Lower concentrations reduce these risks but may give incomplete coupling.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I couple antibodies, enzymes, and aptamers?\u003c\/strong\u003e\u003cbr\u003eYes. Any molecule with primary amine groups (amino acid residues, peptides, antibodies, enzymes, biotin-conjugates, DNA aptamers) can be coupled via EDC. Protein coupling efficiency is typically 60–95% under optimal conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I prevent non-specific binding during capture?\u003c\/strong\u003e\u003cbr\u003eBlock beads post-coupling with 5–10% BSA, casein, or serum in your capture buffer, and include detergent (0.01–0.1% Triton X-100 or Tween-20) and salt (0.2–0.5 M NaCl) in wash buffers. Include appropriate negative control beads (coupled with an irrelevant antibody or protein) in each experiment.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse these beads?\u003c\/strong\u003e\u003cbr\u003eBeads coupled with stable protein attachments (via amide bonds) are difficult to regenerate. For single-use applications, this is not a limitation. For multi-use workflows, design experiments to avoid permanent cross-linking of the beads themselves (e.g., use streptavidin-biotin or other reversible capture chemistry on top of the agarose beads).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDo these beads work with organic solvents?\u003c\/strong\u003e\u003cbr\u003eAgarose is stable in aqueous and aqueous-organic mixtures (up to 50% ethanol or methanol), but extended exposure to higher organic solvent concentrations may swell or degrade the beads. For primarily organic applications, consider polystyrene magnetic beads instead.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3791\/54518\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.omtm.2023.07.010\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003e\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1023\/b:modi.0000006780.36844.64\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52809022406954,"sku":"BTS-B2025449","price":1175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025449.png?v=1790859788","url":"https:\/\/bluetigerscientific.com\/products\/carboxyl-magnetic-agarose-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}