{"product_id":"bovine-serum-albumin-conjugated-to-agarose-beads","title":"Bovine Serum Albumin Conjugated to Agarose Beads","description":"\u003cdiv style=\"max-width:1400px; margin:0 auto; padding:40px 20px; font-family:'Open Sans',sans-serif; font-weight:300; background:#fff; color:#333; font-size:0.95rem; box-sizing:border-box;\"\u003e\n  \u003cdiv style=\"display:flex; flex-direction:column; gap:20px;\"\u003e\n\u003ch2 style=\"margin:0; font-weight:600;\"\u003eBovine Serum Albumin Conjugated to Agarose Beads – Catalog #B2025625\u003c\/h2\u003e\n\u003cp\u003eBSA-Conjugated Agarose Beads (Catalog #B2025625) are an affinity chromatography tool combining bovine serum albumin immobilized on agarose microbeads. Supplied as a 5 mL suspension, these beads provide standard binding capacity for capturing target proteins in pull-down assays, immunoprecipitation, and protein-protein interaction studies. The porous agarose matrix supports rapid mass transfer and high flow rates.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto; max-width:100%; margin-bottom:20px;\"\u003e\n\u003ctable style=\"width:100%; max-width:640px; border-collapse:collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eCatalog number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eB2025625\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLot number:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eExpiration Date:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBatch dependent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eAmount:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eSupplied as:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eSuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eLigand:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBovine serum albumin (BSA)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eMatrix:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAgarose microbeads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eBinding capacity:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eStandard (request COA\/TDS for lot-specific values)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eAffinity purification, protein pull-down assays, immunoprecipitation, protein-protein interaction studies, chromatographic separation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003e2–8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eKeywords:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBSA agarose beads, BSA-conjugated beads, bovine serum albumin beads, affinity purification beads, protein binding beads, agarose chromatography, affinity chromatography media\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #ddd;\"\u003e\n\u003ctd style=\"width:150px; padding:6px 10px 6px 0; vertical-align:top;\"\u003e\u003cstrong\u003eGrade:\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding:6px 0;\"\u003eBiotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity \u0026gt;18 MΩ-cm) and are filtered through 0.22 um.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eScientific Overview\u003c\/h3\u003e\n\u003cp\u003eBovine serum albumin (BSA) is a 66 kDa plasma protein with extensive use as an affinity ligand. When covalently conjugated to agarose, BSA's numerous surface lysine residues and hydrophobic patches enable capture of proteins through electrostatic, hydrophobic, and hydrogen bonding interactions. Agarose, a natural polysaccharide polymer, forms a hydrophilic porous matrix (typical bead diameter 45–165 µm) that supports high protein binding capacity and flow rates suitable for both batch and column-based purification.\u003c\/p\u003e\n\u003cp\u003eThe agarose-BSA conjugate is widely used in affinity chromatography for protein capture, separation, and interaction studies. Its versatility stems from BSA's ability to bind a diverse range of proteins and protein complexes.\u003c\/p\u003e\n\u003cp\u003eKey applications include:\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eAffinity capture of proteins that interact with BSA or BSA-binding partners\u003c\/li\u003e\n\u003cli\u003ePull-down assays for protein-protein interaction studies\u003c\/li\u003e\n\u003cli\u003eImmunoprecipitation of BSA-binding antibodies and immune complexes\u003c\/li\u003e\n\u003cli\u003eDepletion of BSA contaminants in protein mixtures (negative selection)\u003c\/li\u003e\n\u003cli\u003eChromatographic separation of serum proteins and complexes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eUsage \u0026amp; Handling Guidance\u003c\/h3\u003e\n\u003cp\u003eStore the suspension at 2–8°C. Gently resuspend beads by inverting 5–10 times before use; avoid vortexing. Equilibrate beads with binding buffer (PBS or TRIS, pH 7–8) before adding samples.\u003c\/p\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBatch binding:\u003c\/strong\u003e Incubate target protein with beads for 10–30 minutes at room temperature or 4°C. Gently mix periodically to ensure uniform binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWashing:\u003c\/strong\u003e Remove unbound material with 3–5 buffer washes. For stringent conditions, add mild detergent (0.05% Tween-20) or salt.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eElution:\u003c\/strong\u003e Release bound protein using pH shift (0.1 M glycine pH 2.5) or salt increase (1 M NaCl). Capture eluate immediately in pH-neutral buffer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegeneration:\u003c\/strong\u003e Beads can be reused; wash with water, store in 20% ethanol or buffer at 2–8°C. Regeneration capacity depends on elution method and use frequency.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhat You Get\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e5 mL of BSA-conjugated agarose bead suspension\u003c\/li\u003e\n\u003cli\u003eAgarose matrix with standard binding capacity\u003c\/li\u003e\n\u003cli\u003eReady-to-use for immediate batch or column purification\u003c\/li\u003e\n\u003cli\u003eCompatible with automated purification systems\u003c\/li\u003e\n\u003cli\u003eFor research use only (RUO)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eWhy Researchers Choose It\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003eRobust agarose matrix enables reusable, cost-effective purification\u003c\/li\u003e\n\u003cli\u003eExcellent flow characteristics and rapid binding kinetics\u003c\/li\u003e\n\u003cli\u003eVersatile BSA ligand for diverse protein capture applications\u003c\/li\u003e\n\u003cli\u003eSuitable for both batch and column-based workflows\u003c\/li\u003e\n\u003cli\u003eCompatible with standard purification protocols\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 style=\"margin-top:30px;\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cul style=\"padding-left:20px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan I reuse these beads?\u003c\/strong\u003e\u003cbr\u003eYes. After elution, wash beads extensively with water and store at 2–8°C in 20% ethanol or storage buffer. Regeneration capacity depends on elution method; consult lot documentation for cycling limits.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow do I measure binding capacity?\u003c\/strong\u003e\u003cbr\u003eIncubate a known concentration of target protein with beads, measure protein depletion in the supernatant, and calculate the amount bound per mL of beads. Capacity varies by lot and BSA coupling density.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat proteins bind to BSA beads?\u003c\/strong\u003e\u003cbr\u003eMany proteins interact with BSA through electrostatic and hydrophobic forces. Binding strength depends on pH, salt, and protein properties. Pre-test your target protein or consult available protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCan these beads be used in automated systems?\u003c\/strong\u003e\u003cbr\u003eYes. Beads are compatible with most automated protein purification platforms; consult system documentation for bead specifications and flow requirements.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhat is the difference between standard and high-capacity versions?\u003c\/strong\u003e\u003cbr\u003eHigh-capacity beads (Catalog #B2025633) carry higher BSA loading and are suitable for large-scale purification or lower protein concentrations. Use standard (B2025625) for routine work.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin-top:20px; font-weight:bold; color:#c8102e;\"\u003eThis product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/div\u003e\n\u003chr\u003e\n\u003ch4\u003eReferences\u003c\/h4\u003e\n\u003cul style=\"padding-left:0; margin:0; list-style:none;\"\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eKrämer M, Kissmann AK, Raber HF, Xing H, Favella P, Müller I, Spellerberg B, Weil T, Kubiczek D, Sihler S, Ziener U, Rosenau F. BSA Hydrogel Beads Functionalized with a Specific Aptamer Library for Capturing Pseudomonas aeruginosa in Serum and Blood. \u003cem\u003eInt J Mol Sci.\u003c\/em\u003e 2021;22(20).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3390\/ijms222011118\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eLalli E, Silva JS, Boi C, Sarti GC. Affinity Membranes and Monoliths for Protein Purification. \u003cem\u003eMembranes (Basel).\u003c\/em\u003e 2019;10(1).\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.3390\/membranes10010001\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eGerace E, Moazed D. Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads. \u003cem\u003eMethods Enzymol.\u003c\/em\u003e 2015;559:99-110.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/bs.mie.2014.11.010\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli style=\"display:flex; justify-content:space-between; align-items:flex-start; gap:12px; padding:8px 0; border-bottom:1px solid #eee;\"\u003e\n\u003cspan style=\"flex:1;\"\u003eAmiri S, Mehrnia MR, Roudsari FP. Enhancing purification efficiency of affinity functionalized composite agarose micro beads using Fe(3)O(4) nanoparticles. \u003cem\u003eJ Chromatogr B Analyt Technol Biomed Life Sci.\u003c\/em\u003e 2017;1041-1042:27-36.\u003c\/span\u003e\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jchromb.2016.11.035\" target=\"_blank\" rel=\"noopener\" style=\"flex-shrink:0; margin-top:2px;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/Reference.png?v=1775409336\" alt=\"Reference\" style=\"height:28px; width:auto;\"\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Molecular Depot","offers":[{"title":"Default Title","offer_id":52809016705322,"sku":"BTS-B2025625","price":1175.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0896\/1579\/4474\/files\/B2025625.png?v=1790859357","url":"https:\/\/bluetigerscientific.com\/products\/bovine-serum-albumin-conjugated-to-agarose-beads","provider":"Blue Tiger Scientific","version":"1.0","type":"link"}